Bandal, J.N.; Tile, V.A.; Sayyed, R.Z.; Jadhav, H.P.; Azelee, N.I.W.; Danish, S.; Datta, R. Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp. H7 Isolated from Marine Water. Molecules2021, 26, 2833.
Bandal, J.N.; Tile, V.A.; Sayyed, R.Z.; Jadhav, H.P.; Azelee, N.I.W.; Danish, S.; Datta, R. Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp. H7 Isolated from Marine Water. Molecules 2021, 26, 2833.
Bandal, J.N.; Tile, V.A.; Sayyed, R.Z.; Jadhav, H.P.; Azelee, N.I.W.; Danish, S.; Datta, R. Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp. H7 Isolated from Marine Water. Molecules2021, 26, 2833.
Bandal, J.N.; Tile, V.A.; Sayyed, R.Z.; Jadhav, H.P.; Azelee, N.I.W.; Danish, S.; Datta, R. Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp. H7 Isolated from Marine Water. Molecules 2021, 26, 2833.
Abstract
Using the above results from RMS analysis the optimum values were predicted for the independent significant variables (Figure 3) the optimized levels of these variables in combination with other media variables the maximum production was predicted to be 199.90 U/mL. The predicted data were validated through confirmatory experiments performed in triplicates. A 1.29-fold increase in amylase activity against un-optimized (OVAT) medium was achieved in the present study authenticating the efficacy of RSM in process optimization (Figure 4). 2.6 Model validation and scale-up at laboratory scale (5L) bioreactor Once the parameters were standardized in the shake-flasks culture, the experiment was scaled-up to a laboratory-scale bioreactor (5 L). The yield of amylase increased by 1.01 fold (205.69 U/mL), it could be possible because the enzyme production in a bioreactor is higher than in shake-flasks culture as the various critical variable factors such as the dissolved oxygen (DO) and the pH can be optimally controlled at the desired levels [22].
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
