Article
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qPCR System for Alongshan Virus Detection
Version 1
: Received: 10 May 2023 / Approved: 11 May 2023 / Online: 11 May 2023 (14:25:01 CEST)
How to cite: Litov, A.; Okhezin, E.; Kholodilov, I.; Polienko, A.; Karganova, G. qPCR System for Alongshan Virus Detection. Preprints 2023, 2023050869. https://doi.org/10.20944/preprints202305.0869.v1 Litov, A.; Okhezin, E.; Kholodilov, I.; Polienko, A.; Karganova, G. qPCR System for Alongshan Virus Detection. Preprints 2023, 2023050869. https://doi.org/10.20944/preprints202305.0869.v1
Abstract
The recently discovered Jingmenvirus group includes viruses with a segmented genome, RNA of a positive polarity, and several proteins with distant homology to the proteins of the members of the genus Flavivirus. Some Jingmenvirus group members, namely Alongshan virus (ALSV) and Jingmen tick virus, are reported to be tick-borne human pathogens, causing a wide variety of symptoms. ALSV is widely distributed in Eurasia, yet there is no reliable assay for its detection. Here, we describe a qPCR system for the detection of ALSV. Our data show that this system can detect as low as 104 copies of ALSV in the probe. It shows no amplification with common tick-borne viruses circulating in Eurasia, Yanggou tick virus—another member of the Jingmenvirus group—or some known members of the genus Flavivirus. The qPCR system was tested have no non-specific signal for Ixodes ricinus, I. persulcatus, Dermacentor reticulatus, D. marginatus, Haemaphysalis concinna, and H. japonica ticks. Overall, the qPCR system described here can be used for reliable and quantitative ALSV detection.
Keywords
Jingmenvirus group; Alongshan virus; qPCR; Flavivirus; Yanggou tick virus; tick-borne viruses
Subject
Biology and Life Sciences, Virology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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