He, W.; Wu, X.; Tian, L.; Liu, Z.; Xie, D.; Wang, X.; Shen, Q.; Zhu, Y. Enhanced Inhibitory Effect of Chitosan-Ag Nanoparticles Hydrogel on Alternaria alternata in jujube. Preprints2024, 2024050238. https://doi.org/10.20944/preprints202405.0238.v1
APA Style
He, W., Wu, X., Tian, L., Liu, Z., Xie, D., Wang, X., Shen, Q., & Zhu, Y. (2024). Enhanced Inhibitory Effect of Chitosan-Ag Nanoparticles Hydrogel on Alternaria alternata in jujube. Preprints. https://doi.org/10.20944/preprints202405.0238.v1
Chicago/Turabian Style
He, W., Qi Shen and Yajuan Zhu. 2024 "Enhanced Inhibitory Effect of Chitosan-Ag Nanoparticles Hydrogel on Alternaria alternata in jujube" Preprints. https://doi.org/10.20944/preprints202405.0238.v1
Abstract
Silver ion (Ag+) are recognized for their potent antibacterial properties; however, their utilization in food preservation is restricted by factors such as inadequate stability, a transient antibacterial impact, and the potential for human absorption. Hydrogel-based antibacterial agents are capable of exerting their antibacterial effects by establishing appropriate microenvironments, which facilitate the efficient delivery of antimicrobial molecules to the contaminated regions of food. In previous studies, we observed that chitosan (CS) and silver nitrate (AgNO3) were able to reduce Ag+ to silver nanoparticles (AgNPs) with enhanced antibacterial properties. This not only significantly extended the stability of the antibacterial agent but also demonstrated exceptional inhibitory capabilities against pathogenic bacteria, including common grape contaminants such as P. expansum, A. niger, and B. cinerea. Utilizing ultrasound-induced complexation technology, this study has successfully synthesized a chitosan-silver (CS-AgNPs) hydrogel. The efficacy of this hydrogel against the pathogenic fungus responsible for jujube black spot disease has been thoroughly investigated. The morphology structures of CS-AgNPs hydrogels were characterized by TEM, FTIR and UV-vis. The results indicated that AgNPs ranging from 80 to 110 nm were uniformly dispersed within the gel matrix and remained stable for extended periods at room temperature. In vitro antibacterial assays and fractional inhibitory concentration index (FICI) studies revealed that the pronounced antibacterial efficacy of the CS-AgNPs hydrogel was primarily attributed to the additive antibacterial effect of CS and AgNPs. These insights underscore the significant potential of CS-AgNPs hydrogels in combating fungal diseases of jujube, offering innovative strategies and materials for the management of agricultural pathogens.
Keywords
chitosan-Ag nanoparticles; jujube; black spot disease; FICI; additive effect
Subject
Chemistry and Materials Science, Food Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
