Version 1
: Received: 14 May 2024 / Approved: 14 May 2024 / Online: 14 May 2024 (14:33:26 CEST)
How to cite:
Maeda, M.; Murashita, M.; Aoshima, K.; Kobayashi, A.; Fukushi, H.; Kimura, T. Identification of the Promoter Antisense Transcript Enhancing the Transcription of the Equine Herpesvirus 1 Immediate Early Gene. Preprints2024, 2024050974. https://doi.org/10.20944/preprints202405.0974.v1
Maeda, M.; Murashita, M.; Aoshima, K.; Kobayashi, A.; Fukushi, H.; Kimura, T. Identification of the Promoter Antisense Transcript Enhancing the Transcription of the Equine Herpesvirus 1 Immediate Early Gene. Preprints 2024, 2024050974. https://doi.org/10.20944/preprints202405.0974.v1
Maeda, M.; Murashita, M.; Aoshima, K.; Kobayashi, A.; Fukushi, H.; Kimura, T. Identification of the Promoter Antisense Transcript Enhancing the Transcription of the Equine Herpesvirus 1 Immediate Early Gene. Preprints2024, 2024050974. https://doi.org/10.20944/preprints202405.0974.v1
APA Style
Maeda, M., Murashita, M., Aoshima, K., Kobayashi, A., Fukushi, H., & Kimura, T. (2024). Identification of the Promoter Antisense Transcript Enhancing the Transcription of the Equine Herpesvirus 1 Immediate Early Gene. Preprints. https://doi.org/10.20944/preprints202405.0974.v1
Chicago/Turabian Style
Maeda, M., Hideto Fukushi and Takashi Kimura. 2024 "Identification of the Promoter Antisense Transcript Enhancing the Transcription of the Equine Herpesvirus 1 Immediate Early Gene" Preprints. https://doi.org/10.20944/preprints202405.0974.v1
Abstract
Equine herpesvirus-1 (EHV-1) causes respiratory diseases, abortion, and encephalomyelitis in horses. EHV-1 immediate-early (IE) protein, essential for viral replication, is transactivated by the binding of a multiprotein complex including the open reading frame 12 (ORF12) and some host factors to the IE promoter region. Promoter-associated non-coding RNAs (pancRNAs), which are transcribed from bidirectional promoters, regulate the transcription of neighboring genes in mammals and pathogens. In this study, we identified a novel pancRNA transcribed from across the areas of the 5’-untranslated region and promoter of EHV-1 IE and named it IE pancRNA. IE pancRNA and mRNA were simultaneously expressed in EHV-1-infected RN33B-A68B2M cells. This pancRNA was also transcribed in RK13 and E. Derm cells, which are highly susceptible to EHV-1 infection. Furthermore, IE pancRNA upregulated IE gene expression in the presence of ORF12, and stable expression of IE pancRNA increased the number of EHV-1-infected RN33B-A68B2M cells. These results suggest that IE pancRNAs facilitate EHV-1 proliferation by promoting IE gene expression.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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