Article
Version 2
This version is not peer-reviewed
Lipofuscin-Type Pigment as a Marker of Colorectal Cancer
Version 1
: Received: 12 October 2020 / Approved: 14 October 2020 / Online: 14 October 2020 (12:40:15 CEST)
Version 2 : Received: 29 October 2020 / Approved: 30 October 2020 / Online: 30 October 2020 (09:02:45 CET)
Version 2 : Received: 29 October 2020 / Approved: 30 October 2020 / Online: 30 October 2020 (09:02:45 CET)
A peer-reviewed article of this Preprint also exists.
Carvalho, S.; Carneiro, I.; Henrique, R.; Tuchin, V.; Oliveira, L. Lipofuscin-Type Pigment as a Marker of Colorectal Cancer. Electronics 2020, 9, 1805. Carvalho, S.; Carneiro, I.; Henrique, R.; Tuchin, V.; Oliveira, L. Lipofuscin-Type Pigment as a Marker of Colorectal Cancer. Electronics 2020, 9, 1805.
Abstract
The study of the optical properties of biological tissues for a wide spectral range is necessary for the development and planning of noninvasive optical methods to be used in clinical practice. In this study, we propose a new method to calculate almost all optical properties of tissues as a function of wavelength directly from spectral measurements. Using this method and with the exception of the reduced scattering coefficient, which was obtained by traditional simulation methods, all the other optical properties were calculated in a simple and fast manner for human and pathological colorectal tissues. The obtained results are in good agreement with previous published data, both in magnitude and in wavelength dependence. Since this method is based on spectral measurements and not on discrete-wavelength experimental data, the calculated optical properties contain spectral signatures that correspond to major tissue chromophores such as DNA and hemoglobin. Analysis of the absorption bands of hemoglobin in the wavelength dependence of the absorption spectra of normal and pathological colorectal mucosa allowed to identify differentiated accumulation of a pigment in these tissues. The increased content of this pigment in the pathological mucosa may be used for the future development of noninvasive diagnostic methods for colorectal cancer detection.
Keywords
optical properties of tissues; tissue spectroscopy; differentiated pigment content; colorectal cancer; optical cancer detection; absorption coefficient; scattering coefficient; scattering anisotropy; light penetration depth
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Commenter: Luis Oliveira
Commenter's Conflict of Interests: Author
Equation 19 was replaced to match the reported wavelength dependence of the absoption of lipofuscin. Consequently data in graphs of Figure 13 were recalculated.
Some small corrections were made along the text to improve English.
A new reference, 38 in the list was added.