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Hepatitis C Virus E2—Host Cell Receptor, HSPA5, Binding Site Prediction
Version 1
: Received: 26 October 2020 / Approved: 27 October 2020 / Online: 27 October 2020 (09:11:13 CET)
How to cite: Elgohary, A.; Elfiky, A. Hepatitis C Virus E2—Host Cell Receptor, HSPA5, Binding Site Prediction. Preprints 2020, 2020100543. https://doi.org/10.20944/preprints202010.0543.v1 Elgohary, A.; Elfiky, A. Hepatitis C Virus E2—Host Cell Receptor, HSPA5, Binding Site Prediction. Preprints 2020, 2020100543. https://doi.org/10.20944/preprints202010.0543.v1
Abstract
Hepatitis C Virus (HCV) is the main causative factor for liver cirrhosis and the development of liver cancer, with a confirmed ~ 180 million infections worldwide. E2 is an HCV structural protein responsible for virus entry to the host cell. Heat Shock Protein A5 (HSPA5), also termed BiP and GRP78, is the master regulator of the unfolded protein response mechanism, where it mainly localizes in the lumen of the Endoplasmic Reticulum (ER) in normal conditions. Under the stress of HCV infection or carcinogenesis, HSPA5 is upregulated. Consequently, HSPA5 escapes the ER retention localization and translocates to the cytoplasm and plasma membrane. Pep42, a cyclic peptide that was reported to target explicitly cell-surface HSPA5 in vivo. Owing to the high sequence and structural conservation between the C554-C566 region of HCV E2 and the Pep42, then we propose that the HCV E2 C554-C566 region could be the recognition site. The motivation of this work is to predict the possible binding mode between HCV E2 and HSPA5 by implementing molecular docking to test such proposed binding. Docking results reveal the high potent binding of the HCV E2 C554-C566 region to HSPA5 substrate-binding domain β (SBDβ). Moreover, the full-length HCV E2 also exhibits high binding potency to HSPA5 SBDβ. Defining the binding mode between HCV E2 and HSPA5 is of significance, so one can interfere with such binding and reducing the viral infection.
Keywords
HSPA5; GRP78; BiP; HCV E2; protein-protein docking; structural bioinformatics
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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