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Biocide Coatings Against Gram-positive Bacteria From Linear and Branched Polycations. The Decisive Role of the Diffusion Coefficients of Macromolecules
Pigareva, V.A.; Marina, V.I.; Bolshakova, A.V.; Berkovich, A.K.; Kuznetsova, O.A.; Semenova, A.A.; Yushina, Y.K.; Bataeva, D.S.; Grudistova, M.A.; Sybachin, A.V. Biocidal Coatings against Gram-Positive Bacteria from Linear and Branched Polycations: The Decisive Role of the Diffusion Coefficients of Macromolecules. Coatings2023, 13, 1076.
Pigareva, V.A.; Marina, V.I.; Bolshakova, A.V.; Berkovich, A.K.; Kuznetsova, O.A.; Semenova, A.A.; Yushina, Y.K.; Bataeva, D.S.; Grudistova, M.A.; Sybachin, A.V. Biocidal Coatings against Gram-Positive Bacteria from Linear and Branched Polycations: The Decisive Role of the Diffusion Coefficients of Macromolecules. Coatings 2023, 13, 1076.
Pigareva, V.A.; Marina, V.I.; Bolshakova, A.V.; Berkovich, A.K.; Kuznetsova, O.A.; Semenova, A.A.; Yushina, Y.K.; Bataeva, D.S.; Grudistova, M.A.; Sybachin, A.V. Biocidal Coatings against Gram-Positive Bacteria from Linear and Branched Polycations: The Decisive Role of the Diffusion Coefficients of Macromolecules. Coatings2023, 13, 1076.
Pigareva, V.A.; Marina, V.I.; Bolshakova, A.V.; Berkovich, A.K.; Kuznetsova, O.A.; Semenova, A.A.; Yushina, Y.K.; Bataeva, D.S.; Grudistova, M.A.; Sybachin, A.V. Biocidal Coatings against Gram-Positive Bacteria from Linear and Branched Polycations: The Decisive Role of the Diffusion Coefficients of Macromolecules. Coatings 2023, 13, 1076.
Abstract
Positively charged polyelectrolytes hold significant potential as materials for creating antibacterial coatings. Water-soluble macromolecules can be easily deposited onto a treated surface through various techniques, forming a film that provides antimicrobial properties to the surface through electrostatic interactions with bacterial membranes. The structural and molecular weight characteristics of the polymers play a crucial role in the physical and mechanical properties of the coatings. In this study, we investigated the properties of branched polyethyleneimine (PEI) and linear polydiallyldimethylammonium chloride (PDADMAC) coatings with different molecular weights of polymer samples to determine their suitability as stable coatings with antibacterial properties against foodborne bacteria. Using thermogravimetric analysis, dynamometry, and dynamic light scattering, we examined the physicochemical and mechanical properties of the macromolecules in water solutions and in coatings. Microbiological study was conducted to analyze the biocidal activity of the polycations in solutions and on the surface of coatings. Our findings showed that the moisture saturation of the polycationic coatings did not significantly depend on the chemical nature of charged groups or the molecular weight or architecture of macromolecules. Moreover, biocidal activity was not found to depend on the molecular weights of PEI and PDADMAC. However, the lowering of the molecular weight of polymers resulted in the loss of cohesive forces in the coatings and to dramatically loss of the stability towards wash-off with water. The diffusion coefficient (D0) of macromolecules was identified as a key parameter for the wash-off mechanism. Films formed by molecules with D0 below 1×10-7 cm2/s demonstrated high resistance to wash-off procedures. We demonstrated that PEI and PDADMAC samples with high molecular weights showed high antimicrobial activity towards L. monocytogenes. Our results highlight the importance of macromolecule characteristics in the development of new biocidal coatings based on polycations.
Chemistry and Materials Science, Surfaces, Coatings and Films
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