Review
Version 1
Preserved in Portico This version is not peer-reviewed
Crispr-cas System: Classification, Benefits, Applications And Function
Version 1
: Received: 1 February 2024 / Approved: 1 February 2024 / Online: 1 February 2024 (14:24:57 CET)
How to cite: Nazir, T.; A. Mirza, H.; Taha, N. Crispr-cas System: Classification, Benefits, Applications And Function. Preprints 2024, 2024020085. https://doi.org/10.20944/preprints202402.0085.v1 Nazir, T.; A. Mirza, H.; Taha, N. Crispr-cas System: Classification, Benefits, Applications And Function. Preprints 2024, 2024020085. https://doi.org/10.20944/preprints202402.0085.v1
Abstract
Because it can make exact double-strand breaks in DNA, CRISPR-Cas9 can help almost every species and type of cell. It is a powerful way to change the DNA code. CRISPR-Cas9 can be used for a lot more than just changing genes. We are close to being able to do high-throughput gene screening, epigenome editing, in-vivo cell tagging, and RNA change. With the help of CRISPR-Cas9, the function of genes can be studied better, and more realistic disease models can be made. The revolutionary new discipline of gene editing has the potential to profoundly impact healthcare and the life sciences. By simplifying the process of creating double-strand breaks in the DNA of almost any species or kind of cell, CRISPR-Cas9 has revolutionized gene editing. Numerous applications have been found for the CRISPR-Cas9 system. High-throughput gene screening, RNA modification, live-cell chromosome marking, and epigenome editing are all examples. CRISPR-Cas9 facilitates gene research, leading to the development of CRISPR-based disease models. CRISPR-Cas9-based methods of altering the genome will aid researchers in learning more about sickness and discovering better ways to cure it, despite the fact that there are still many questions and large difficulties to answer.
Keywords
CRISPR-Cas System, Biomedicine, Genome engineering, Gene editing, Human disease, Cancer, Animal models Concerns, Benefits, Applications, Function
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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