Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Populus euphratica CPK21 Interact with NF-YC3 to Enhance Cadmium Tolerance in Arabidopsis

Kexin Yin
,
Yi Liu
,
Zhe Liu
,
Rui Zhao
,
Ying Zhang
,
Caixia Yan
,
Ziyan Zhao
,
Bing Feng
,
Xiaomeng Zhang
,
Keyue An
,
Jing Li
,
Jian Liu
,
Kaiyue Dong
,
Jun Yao
,
Nan Zhao
,
Xiaoyang Zhou
,
Shaoliang Chen
* ORCID logo
Version 1 : Received: 1 May 2024 / Approved: 2 May 2024 / Online: 2 May 2024 (09:49:43 CEST)

How to cite: Yin, K.; Liu, Y.; Liu, Z.; Zhao, R.; Zhang, Y.; Yan, C.; Zhao, Z.; Feng, B.; Zhang, X.; An, K.; Li, J.; Liu, J.; Dong, K.; Yao, J.; Zhao, N.; Zhou, X.; Chen, S. Populus euphratica CPK21 Interact with NF-YC3 to Enhance Cadmium Tolerance in Arabidopsis. Preprints 2024, 2024050108. https://doi.org/10.20944/preprints202405.0108.v1 Yin, K.; Liu, Y.; Liu, Z.; Zhao, R.; Zhang, Y.; Yan, C.; Zhao, Z.; Feng, B.; Zhang, X.; An, K.; Li, J.; Liu, J.; Dong, K.; Yao, J.; Zhao, N.; Zhou, X.; Chen, S. Populus euphratica CPK21 Interact with NF-YC3 to Enhance Cadmium Tolerance in Arabidopsis. Preprints 2024, 2024050108. https://doi.org/10.20944/preprints202405.0108.v1

Abstract

The toxic cadmium (Cd) poses a serious threat to plant growth and human health. Populus euphratica CPK21 has previously been shown to attenuate Cd toxicity by reducing Cd accumulation, enhancing antioxidant defense and improving water balance in transgenic Arabidopsis. Here, we confirmed a protein-protein interaction between PeCPK21 and AtNF-YC3 by yeast two-hybrid, HaloTag pull-down and bimolecular fluorescence complementation assays. AtNF-YC3 was induced by Cd and strongly expressed in PeCPK21-overexpressed plants. Overexpression of AtNF-YC3 in Arabidopsis reduced salt inhibition of root length, fresh weight and membrane stability under Cd stress conditions (100 µM, 7 d), suggesting that AtNF-YC3 appears to contribute to the improvement of Cd stress tolerance. AtNF-YC3 improved Cd tolerance by limiting Cd uptake and accumulation, activating antioxidant enzymes, and reducing H2O2 production under Cd stress. We conclude that PeCPK21 interacts with AtNF-YC3 to limit Cd accumulation, enhance the ROS scavenging system, and thereby positively regulate plant adaptation to Cd environments. This study highlights the interaction between PeCPK21 and AtNF-YC3 under Cd stress conditions, which can be utilized to improve Cd tolerance in higher plants.

Keywords

Populus euphratica; PeCPK21; AtNF-YC3; Cadmium; Cd fluxes; H2O2; enzyme activities

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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