Version 1
: Received: 5 May 2024 / Approved: 6 May 2024 / Online: 6 May 2024 (07:37:30 CEST)
How to cite:
Kim, Y.; Yun, B.; Ye, B. S.; Kim, B.-Y. Generation of an Alzheimer’s Disease Model Derived from Induced Pluripotent Stem Cells with an APP Gene Mutation. Preprints2024, 2024050243. https://doi.org/10.20944/preprints202405.0243.v1
Kim, Y.; Yun, B.; Ye, B. S.; Kim, B.-Y. Generation of an Alzheimer’s Disease Model Derived from Induced Pluripotent Stem Cells with an APP Gene Mutation. Preprints 2024, 2024050243. https://doi.org/10.20944/preprints202405.0243.v1
Kim, Y.; Yun, B.; Ye, B. S.; Kim, B.-Y. Generation of an Alzheimer’s Disease Model Derived from Induced Pluripotent Stem Cells with an APP Gene Mutation. Preprints2024, 2024050243. https://doi.org/10.20944/preprints202405.0243.v1
APA Style
Kim, Y., Yun, B., Ye, B. S., & Kim, B. Y. (2024). Generation of an Alzheimer’s Disease Model Derived from Induced Pluripotent Stem Cells with an APP Gene Mutation. Preprints. https://doi.org/10.20944/preprints202405.0243.v1
Chicago/Turabian Style
Kim, Y., Byoung Seok Ye and Bo-Young Kim. 2024 "Generation of an Alzheimer’s Disease Model Derived from Induced Pluripotent Stem Cells with an APP Gene Mutation" Preprints. https://doi.org/10.20944/preprints202405.0243.v1
Abstract
Alzheimer’s disease (AD), the most common cause of dementia, is characterized by disruptions in memory, cognition, and personality, significantly impacting morbidity and mortality rates among older adults. However, the exact pathophysiological mechanism of AD remains unknown, and effective treatment options for AD are still lacking. Human induced pluripotent stem cells (iPSC) are emerging as promising platforms for disease research, offering the ability to model genetic mutations associated with various conditions. Patient-derived iPSCs are useful for modeling neurodegenerative and neurodevelopmental disorders. In this study, we generated AD iPSCs from peripheral blood mononuclear cells obtained from a 65-year-old patient with AD carrying the E682K mutation in the amyloid precursor protein (APP) gene. Cerebral organoids derived from AD iPSCs recapitulated the AD phenotype, exhibiting significantly increased levels of tau protein. Our analysis revealed that an iPSC disease model of AD is a valuable assessment tool for pathophysiological research and drug screening.
Keywords
Alzheimer’s disease; amyloid precursor protein; cerebral organoid; disease modeling; drug screening; induced pluripotent stem cells; organoids; tau pathology
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
