Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Chemical Content and Cytotoxic Activity on Various Cancer Cell Lines of Chaga (Inonotus obliquus) Growing on Betula pendula and Betula pubescens

Ain Raal
* ORCID logo ,
Hedi Kaldmäe
,
Karin Kütt
,
Katrin Jürimaa
,
Maidu Silm
,
Uko Bleive
,
Alar Aluvee
,
Kalev Adamson
ORCID logo ,
Marili Vester
ORCID logo ,
Mart Erik
,
Oleh Koshovyi
,
Khan Viet Nguyen
,
Hoai Thi Nguyen
,
Rein Drenkhan
Version 1 : Received: 6 May 2024 / Approved: 7 May 2024 / Online: 8 May 2024 (09:48:08 CEST)

How to cite: Raal, A.; Kaldmäe, H.; Kütt, K.; Jürimaa, K.; Silm, M.; Bleive, U.; Aluvee, A.; Adamson, K.; Vester, M.; Erik, M.; Koshovyi, O.; Nguyen, K. V.; Nguyen, H. T.; Drenkhan, R. Chemical Content and Cytotoxic Activity on Various Cancer Cell Lines of Chaga (Inonotus obliquus) Growing on Betula pendula and Betula pubescens. Preprints 2024, 2024050419. https://doi.org/10.20944/preprints202405.0419.v1 Raal, A.; Kaldmäe, H.; Kütt, K.; Jürimaa, K.; Silm, M.; Bleive, U.; Aluvee, A.; Adamson, K.; Vester, M.; Erik, M.; Koshovyi, O.; Nguyen, K. V.; Nguyen, H. T.; Drenkhan, R. Chemical Content and Cytotoxic Activity on Various Cancer Cell Lines of Chaga (Inonotus obliquus) Growing on Betula pendula and Betula pubescens. Preprints 2024, 2024050419. https://doi.org/10.20944/preprints202405.0419.v1

Abstract

Chaga (Inonotus obliquus) is a pathogenic fungus that grows mostly on birch species (Betula pendula and B. pubescens) and has traditionally been used as an anti-cancer medicine. The study aimed to compare the chemical composition and cytotoxic activity of chagas growing on both Betula spp. on various cancer cell lines. The freeze dried extracts contained triterpenes inotodiol, lanosterol betulin and betulinic acid typical to conks growing on Betula species. The cytotoxic activity of chaga growing on Betula pendula and B. pubescens 80% ethanolic extracts against thirty-one human cancer cell lines has been evaluated by a sulforhodamine B assay. The strongest inhibitions were observed with chaga (growing on B. pendula) extract on the HepG2 and CAL-62 cell line, and with chaga (from B. pubescens) extract on HepG2 cell line, with IC50 values of 37.71, 43.30, and 49.99 μg/mL, respectively. The chaga extracts from B. pendula exert somewhat stronger effects on most cancer cell lines studied than B. pubescens extracts. This study highlight the potential of chaga as a source of bioactive compounds with selective anti-cancer properties, encouraging further exploration of its therapeutic mechanisms and potential applications in cancer treatment.

Keywords

Betula pendula; Betula pubescens; birch; cancer, cell lines; chaga; Estonia; glucans; Inonotus obliquus; triterpenoids

Subject

Medicine and Pharmacology, Pharmacy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.