Version 1
: Received: 8 May 2024 / Approved: 8 May 2024 / Online: 8 May 2024 (15:31:32 CEST)
How to cite:
Burgermeister, S.; Stoykova, S.; Krebs, F. S.; Zoete, V.; Mbefo, M.; Egervari, K.; Reinhard, A.; Bisig, B.; Hewer, E. Methylation-based Characterization of a new IDH2 Mutation in Sinonasal Undifferentiated Carcinoma. Preprints2024, 2024050512. https://doi.org/10.20944/preprints202405.0512.v1
Burgermeister, S.; Stoykova, S.; Krebs, F. S.; Zoete, V.; Mbefo, M.; Egervari, K.; Reinhard, A.; Bisig, B.; Hewer, E. Methylation-based Characterization of a new IDH2 Mutation in Sinonasal Undifferentiated Carcinoma. Preprints 2024, 2024050512. https://doi.org/10.20944/preprints202405.0512.v1
Burgermeister, S.; Stoykova, S.; Krebs, F. S.; Zoete, V.; Mbefo, M.; Egervari, K.; Reinhard, A.; Bisig, B.; Hewer, E. Methylation-based Characterization of a new IDH2 Mutation in Sinonasal Undifferentiated Carcinoma. Preprints2024, 2024050512. https://doi.org/10.20944/preprints202405.0512.v1
APA Style
Burgermeister, S., Stoykova, S., Krebs, F. S., Zoete, V., Mbefo, M., Egervari, K., Reinhard, A., Bisig, B., & Hewer, E. (2024). Methylation-based Characterization of a new IDH2 Mutation in Sinonasal Undifferentiated Carcinoma. Preprints. https://doi.org/10.20944/preprints202405.0512.v1
Chicago/Turabian Style
Burgermeister, S., Bettina Bisig and Ekkehard Hewer. 2024 "Methylation-based Characterization of a new IDH2 Mutation in Sinonasal Undifferentiated Carcinoma" Preprints. https://doi.org/10.20944/preprints202405.0512.v1
Abstract
Mutations affecting codon 172 of the isocitrate dehydrogenase 2 (IDH2) gene define a subgroup of sinonasal undifferentiated carcinomas (SNUCs) with a relatively favorable prognosis and a globally hypermethylated phenotype. They are also recurrent (along with IDH1 mutations) in gliomas, acute myeloid leukemia, and intrahepatic cholangiocarcinoma. Commonly reported mutations, all associated with aberrant IDH2 enzymatic activity, include R172S, R172T, R172G, and R172M. We present a case of SNUC with a never-before-described IDH2 mutation, R172A. Our report compares the methylation pattern of our sample to other cases from the GEO database. Hierarchical clustering suggests a strong association between our sample and other IDH-mutant SNUCs and a clear distinction between sino-nasal normal tissues and tumors. Principal component analysis (PCA), using 100 principal components explaining 94.5% of the variance, shows the position of our sample within 1.02 standard deviation of the other IDH-mutant SNUCs. Molecular modeling analysis of the IDH2 R172A versus other R172 variants provides a structural explanation to how they affect the protein active site. Our findings thus suggest that the R172A mutation in IDH2 confers a gain of function similar to other R172 mutations in IDH2, resulting in a similar hypermethylated profile.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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