Version 1
: Received: 20 May 2024 / Approved: 21 May 2024 / Online: 21 May 2024 (11:55:01 CEST)
How to cite:
Sindeeva, O. A.; Demina, P. A.; Kozyreva, Z. V.; Terentyeva, D. A.; Gusliakova, O. I.; Muslimov, A. R.; Sukhorukov, G. B. Tracking Single Mouse Mesenchymal Stromal Cells Migration into Glioblastoma using a Photoconvertible Microcapsules. Preprints2024, 2024051383. https://doi.org/10.20944/preprints202405.1383.v1
Sindeeva, O. A.; Demina, P. A.; Kozyreva, Z. V.; Terentyeva, D. A.; Gusliakova, O. I.; Muslimov, A. R.; Sukhorukov, G. B. Tracking Single Mouse Mesenchymal Stromal Cells Migration into Glioblastoma using a Photoconvertible Microcapsules. Preprints 2024, 2024051383. https://doi.org/10.20944/preprints202405.1383.v1
Sindeeva, O. A.; Demina, P. A.; Kozyreva, Z. V.; Terentyeva, D. A.; Gusliakova, O. I.; Muslimov, A. R.; Sukhorukov, G. B. Tracking Single Mouse Mesenchymal Stromal Cells Migration into Glioblastoma using a Photoconvertible Microcapsules. Preprints2024, 2024051383. https://doi.org/10.20944/preprints202405.1383.v1
APA Style
Sindeeva, O. A., Demina, P. A., Kozyreva, Z. V., Terentyeva, D. A., Gusliakova, O. I., Muslimov, A. R., & Sukhorukov, G. B. (2024). Tracking Single Mouse Mesenchymal Stromal Cells Migration into Glioblastoma using a Photoconvertible Microcapsules. Preprints. https://doi.org/10.20944/preprints202405.1383.v1
Chicago/Turabian Style
Sindeeva, O. A., Albert R Muslimov and Gleb B Sukhorukov. 2024 "Tracking Single Mouse Mesenchymal Stromal Cells Migration into Glioblastoma using a Photoconvertible Microcapsules" Preprints. https://doi.org/10.20944/preprints202405.1383.v1
Abstract
To elucidate mesenchymal stromal cells (MSC)-tumor intricate and ambiguous interactions, the reliable cell labeling and tracking techniques are imperative. Traditional methods of cell labelling have limitations mostly relevant to need for genetic modification what demands the exploration for alternative strategies. Here, we propose using fluorescent photoconvertible microcapsules containing Rhodamine B (RhB) to study mMSC migration in brain tumors. These 3 μm sized microcapsules are readily internalised by cells and undergo photoconversion under 561 nm laser exposure with fluorescence blue shift on demand. Optimal photoconversion was achieved with a laser irradiation duration of 0.4 ms, which provide a maximal brightness of photoconverted label without an excessive laser exposure on cells. Capsules modified with polyallylamine hydrochloride demonstrated the best potential for intracellular uptake without significantly affecting cell viability, motility, or proliferation. The optimal ratio of 20 capsules per mMSC was determined for labeling. Moreover, migration of individual mMSCs within 2D and 3D glioblastoma cell (EPNT-5) colonies over 2 days and in vivo tumor settings over 7 days was tracked. Our study unveils a robust platform for investigating MSC-tumor dynamics, offering insights into therapeutic strategies. Photoconvertible microcapsules could also become an indispensable tool for studying complex fundamental processes of cell-cell interactions for a wide range of problems in biology and medicine.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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