An infestation of parkinsonia (Parkinsonia aculeata) located on Alexandria Station, Northern Territory, Australia was successfully treated with a bioherbicide using stem implanted capsules. The bioherbicide containing three endemic endophytic fungi (Lasiodiplodia pseudotheobromae, Macrophomina phaseolina and Neoscytalidium novaehollan-diae) is the first Australian registered woody weed bioherbicide. The product was effectively administered to the plant stems using a mechanical device, resulting in subsequent development of a dieback event, which, after a period of establishment, has moved through the adjacent untreated plant population resulting in significant decline in infestation vigour and reduced recruitment. This is the first report of large-scale management of parkinsonia by this method.