Subject:
Biology And Life Sciences,
Anatomy And Physiology
Keywords:
diseases; prevalence; parasitic diseases; pigeon
Online: 16 December 2020 (13:11:26 CET)
This experiment was carried out to determine the overall prevalence of pigeon diseases and especially emphasized on prevalence of parasitic diseases in study areas. During this study 45 farms were selected which have >20 pair of pigeon for commercial and >10 pair pigeon for traditional farm in the three study area namely Rajshahi, Natore and Pabna district of Bangladesh. The data were collected from different farm within the study area through a questionnaire and interview schedule with the farm owners. During one year of study period a total Out of 3677 pigeon, positive case in cage of external parasites were 722 and internal parasite positives 114 from 263 fecal sample. The overall prevalence of gastrointestinal and external parasites in pigeon at northern Bangladesh is obtained. The infestation of lice Columbaecula columbae and fly Pseudolynchia canariensis were observed 18.08% and 1.55% respectively. The present study found two species of ectoparasites lice and fly. Diversity of ectoparasite in birds infestation may be depend on many factors, which may include home range, behavior, size and roosting habit of the host. The overall prevalence of gastrointestinal parasites in pigeon are founded that 43.34% in research area. Specific incidence was 22.81% in Ascariasis, 18.63% in Capilariasis and 1.90% in Raillietina parasite in pigeon. Ascaridia colombae is one of the most common (22.81%) helminth species in pigeons in this study. The effect of breed of pigeon observed in present studies the highest in indigenous (63.47%) and lowest exotic (36%) prevalence of gastrointestinal parasite was observed. The specific percentages of Ascariasis, Capilariasis and Raillatina gastrointestinal parasites of pigeon were found 20% & 31.74%; 14% & 30.15% and 2% & 1.58% in exotic & indigenous breed, respectively. In the present study, there is a relation between the occurrence of gastrointestinal parasitic infections with the age of the pigeons which showed that high prevalence in adults (50%) and compare to young (22.22%). but it was statistically significant (P>0.05). In relation with farming system the gastrointestinal parasitic incidence of pigeon was observed in modern and tradition farming system, the highest incidence was 71.16% in traditional & lowest 31.14% in modern farming. When observed specifically in Ascariasis, Capillariasis & Raillietina were 16.39% & 37.41%; 13.11% & 31.25% and 1.63% & 2.5% in modern & traditional, respectively. The effect of regions on the prevalence of gastrointestinal parasites in pigeon was observed in Pabna (68.32%), Natore (57.84%) and Rajshahi (33.33%). The specific percentages of Ascariasis, Capilariasis and Raillatina gastrointestinal parasites of pigeon were 18.18%, 33.33% & 26.31%; 14.54%, 33.33% & 23.38% and 0.606%, 1.66% & 7.89% in Rajshahi, Pabna and Natore, respectively.
Subject:
Biology And Life Sciences,
Biology And Biotechnology
Keywords:
pigeon pea; bacterial diversity; metataxonomic analysis
Online: 16 October 2023 (13:15:21 CEST)
: With the increase in demand for non-dairy starter cultures and probiotic bacteria as carriers, the use of legumes (also called pulses) as an alternative has gained momentum. In this study, we investigated the diversity of bacterial communities in samples of pigeon pea (Cajanus cajan L. Millsp.) soaked in water for 12 h and 24 h. We soaked 500 g of pigeon pea in sterile distilled water at room temperature (± 25 °C) for 12 h and 24 h; 10 mL of the soaking water was then collected to measure the bacterial diversity using a metataxonomic analysis. The V1–V9 regions on the 16S ribosomal RNA gene were amplified using 27F and 1492R primers under specific polymerase chain reaction conditions for the bacterial identification. Genomic DNA (130 ng) was sequenced on a R9.4 flow cell by Oxford Nanopore Technologies using a GridION sequencer. Library preparations were initiated using a Native Barcoding Kit 24 V14 (SQK-NBD114.24). Primary data were acquired using MinKNOW version 22.05.7. A total of 13 bacterial families and 89 genera were identified in the pigeon pea sample soaked for 12 h; 26 families and 90 genera were identified in the pigeon pea sample soaked for 24 h. Among the bacterial families identified, the five predominant families in both samples were Enterobacteriaceae, Erwiniaceae, Yersiniaceae, Pectobacteriaceae, and Lactobacillaceae. According to the relative abundance of the identified bacterial genera, the following nine genera were predominant in both samples: Enterobacter, Klebsiella, Citrobacter, Pantoea, Kosakonia, Pseudoenterobacter, Pluralibacter, Leclercia, and Kluyvera. At a genus level, a slight increase in the abundance of Klebsiella, Kosakonia, and Pluralibacter and a slight decrease in the abundance of Citrobacter were observed after prolonged incubation from 12 h to 24 h. The values of five diversity indices revealed that the sample soaked in water for 24 h had a richer bacterial abundance and diversity than the 12 h sample. Shannon and Simpson values revealed a higher bacterial diversity in the sample collected at 24 h than the sample collected at 12 h. Species observations and abundance-based coverage estimator (ACE) values demonstrated that the sample collected at 24 h harbored a higher bacterial richness than the sample collected at 12 h. These findings indicated that the bacterial diversity in the pigeon pea samples increased with the soaking time. The bacterial communities during the soaking of the pigeon pea samples were dominated by the Enterobacteriaceae family and Enterobacter genus. The presence of bacterial genera such as Lacticaseibacillus, Lentilactobacillus, and Secundilactobacillus was notable because of their importance as starter cultures for fermented plant-based milk products, including pigeon pea beverages for lactose-intolerant individuals or individuals with malnutrition.