Version 1
: Received: 16 May 2024 / Approved: 17 May 2024 / Online: 17 May 2024 (15:34:58 CEST)
How to cite:
dos Santos, C. M.; Baldivia, D. D. S.; de Castro, D. T. H.; Carvalho, J. T. D. G.; Oliveira, A. S.; da Rocha, P. D. S.; Campos, J. F.; Balogun, S. O.; De Oliveira, C. F. R.; da Silva, D. B.; Carollo, C. A.; De Picoli Souza, K.; dos Santos, E. L. Chemical Composition, Antioxidant, and Cytotoxic Effects of Senna rugosa Leaf and Root Extracts on Human Leukemia Cell Lines. Preprints2024, 2024051172. https://doi.org/10.20944/preprints202405.1172.v1
dos Santos, C. M.; Baldivia, D. D. S.; de Castro, D. T. H.; Carvalho, J. T. D. G.; Oliveira, A. S.; da Rocha, P. D. S.; Campos, J. F.; Balogun, S. O.; De Oliveira, C. F. R.; da Silva, D. B.; Carollo, C. A.; De Picoli Souza, K.; dos Santos, E. L. Chemical Composition, Antioxidant, and Cytotoxic Effects of Senna rugosa Leaf and Root Extracts on Human Leukemia Cell Lines. Preprints 2024, 2024051172. https://doi.org/10.20944/preprints202405.1172.v1
dos Santos, C. M.; Baldivia, D. D. S.; de Castro, D. T. H.; Carvalho, J. T. D. G.; Oliveira, A. S.; da Rocha, P. D. S.; Campos, J. F.; Balogun, S. O.; De Oliveira, C. F. R.; da Silva, D. B.; Carollo, C. A.; De Picoli Souza, K.; dos Santos, E. L. Chemical Composition, Antioxidant, and Cytotoxic Effects of Senna rugosa Leaf and Root Extracts on Human Leukemia Cell Lines. Preprints2024, 2024051172. https://doi.org/10.20944/preprints202405.1172.v1
APA Style
dos Santos, C. M., Baldivia, D. D. S., de Castro, D. T. H., Carvalho, J. T. D. G., Oliveira, A. S., da Rocha, P. D. S., Campos, J. F., Balogun, S. O., De Oliveira, C. F. R., da Silva, D. B., Carollo, C. A., De Picoli Souza, K., & dos Santos, E. L. (2024). Chemical Composition, Antioxidant, and Cytotoxic Effects of <em>Senna rugosa</em> Leaf and Root Extracts on Human Leukemia Cell Lines. Preprints. https://doi.org/10.20944/preprints202405.1172.v1
Chicago/Turabian Style
dos Santos, C. M., Kely De Picoli Souza and Edson Lucas dos Santos. 2024 "Chemical Composition, Antioxidant, and Cytotoxic Effects of <em>Senna rugosa</em> Leaf and Root Extracts on Human Leukemia Cell Lines" Preprints. https://doi.org/10.20944/preprints202405.1172.v1
Abstract
Senna rugosa is a species found in the Cerrado and used in folk medicine as a vermifuge and in the treatment of poisonous snakebites accidents. In this work, we identified the main secondary metabolites present in ethanolic extracts of the leaves (ELSR) and roots (ERSR) of S. rugosa and evaluated the potential cytoprotective effect against cellular macromolecular damage, as well as the cytotoxic properties of the extracts on the K562 and Jurkat leukemic cell lines. The identification of metabolites was done by liquid chromatography coupled with mass spectrometry. The antioxidant activities were investigated by direct ABTS•+ and DPPH• radical scavenging methods, protection against oxidative damage in proteins and DNA. The cytotoxic properties were investigated against healthy cells, isolated from human peripheral blood (PBMC) and leukemic cell lines. The leaf extract contains catechin, rutin, epigallocatechin derivatives, kaempferol glycosides, luteolin, and dimeric and trimeric procyanidins, while the root extract profile shows obtusichromoneside derivatives, 2-methoxystypandrone, stilbene derivatives, naphthopyranones, and flavanone derivatives. The extracts showed antioxidant activity, with IC50 of 4.86 ± 0.51 µg/mL and 8.33 ± 0.90 µg/mL in the ABTS assay for ELSR and ERSR, respectively. And in the DPPH• assay, the IC50 was 19.98 ± 1.96 µg/mL for ELSR and 13.37 ± 1.05 µg/mL for ERSR. The extracts protected macromolecules against oxidative damage at concentrations of 5 µg/mL. The cytotoxicity test against leukemic strains was observed after 24 and 48 hours of treatment. After 48 h, results against the K562 cell line demonstrate IC50 of 242.54 ± 2.38 µg/mL and 223.00 ± 2.34 µg/mL for ELSR and ERSR , respectively. While against the Jurkat cell line, these extracts showed IC50 of 171.45 ± 2.25 µg/mL and 189.30 ± 2.27 µg/mL, respectively Results on PBMC viability, demonstrate that the extracts showed selectivity for the leukemic cell lines. Together, our results reveal that the leaves and roots of S. rugosa have completely distinct and complex chemical compositions. This study expands the repertoire of identified chemical compounds and underscores their significant pharmacological potential in conditions related to oxidative stress and leukemia.
Keywords
Natural Products; Anticancer Properties; Cancer; medicinal plants; Chemical profile
Subject
Biology and Life Sciences, Life Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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