We thank the Light Microscopy Center (LMC) at ETH Zurich for providing excellent imaging facilities and technical support and Ellen Ragan for proofreading. We would also like to thank Dr. Kenneth Yamada at NIH/NIDCR, Bethesda, MD for his kind gift of the mAb13 antibody. Light Microscopy Center ETH Zurich Ellen Ragan Kenneth Yamada NIH/NIDCR mAb13 antibody Light Microscopy Center ETH Zurich Ellen Ragan Kenneth Yamada NIH/NIDCR mAb13 antibody Light Microscopy Center ETH Zurich Ellen Ragan Kenneth Yamada NIH/NIDCR mAb13 antibody We are grateful to Kathy Borkovich and Gloria Turner for antibodies to N. crassa arginase. Kathy Borkovich Gloria Turner antibodies N. crassa arginase Kathy Borkovich Gloria Turner antibodies N. crassa arginase We thank Sue Wormsley and PharMingen (now, BD Biosciences) for the generous gift of baculovirus-infected cells containing recombinant cyclin B1; Mike Sramkoski for assistance with the cytometer; Vince Shankey for cyclin A2 antibody, and Tom Radivoyevitch for helpful suggestions and mathematical advice. Sue Wormsley PharMingen baculovirus-infected cells Mike Sramkoski Vince Shankey cyclin A2 antibody Tom Radivoyevitch We thank Yue Ma and Jingyun Li for excellent technical assistance and Dorothy Xing for providing monoclonal antibodies against Fim2 (NIBSC 04/154) and Fim3 (NIBSC 04/156). This work was supported by the National Science & Technology Pillar Program from the Ministry of Science and Technology, China (no. 2008BAI54B03) and in part by the Academy of Finland. Yue Ma Jingyun Li Dorothy Xing antibodies Fim2 Fim3 National Science & Technology Pillar Program from the Ministry of Science and Technology Academy of Finland Yue Ma Jingyun Li Dorothy Xing monoclonal antibodies Fim2 (NIBSC 04/154) Fim3 (NIBSC 04/156) National Science & Technology Pillar Program from the Ministry of Science and Technology Academy of Finland Yue Ma Jingyun Li Dorothy Xing monoclonal antibodies NIBSC 04/154 NIBSC 04/156 National Science & Technology Pillar Program from the Ministry of Science and Technology Academy of Finland This work was supported by NIH R01 NS 077774 and P30 HD 06979. We are also grateful to Drs. Thomas Smith, Charlie Stanley, and Marc Yudkoff for their helpful comments and advice throughout this study. We would like to thank Elizabeth Krizman for her help teaching Brendan S. Whitelaw the transport assays and her help with the analyses of the subcellular fractionation. We would like to thank Dr. Virginia Lee and Dr. Jeffery D. Rothstein for their generous provision of the anti-GFAP antibody and the anti-GLT-1 antibody, respectively. Thomas Smith Charlie Stanley Marc Yudkoff Elizabeth Krizman Brendan S. Whitelaw Virginia Lee Jeffery D. Rothstein anti-GFAP antibody anti-GLT1 antibody NIH Thomas Smith Charlie Stanley Marc Yudkoff Elizabeth Krizman Brendan S. Whitelaw Virginia Lee Jeffery D. Rothstein anti-GFAP antibody anti-GLT1 antibody We acknowledge our grant sponsors Association pour la Recherche sur le Cancer (ARC) and Ligue Nationale contre le Cancer (Comité de l'Isère). SZ received a grant from UNESCO and ARC. We express our thanks to Dr James A Raleigh for kindly supplying pimonidazole-antibody and to Dr Jonathan Coles for his judicious comments. Association pour la Recherche sur le Cancer Ligue Nationale contre le Cancer (Comité de l'Isère) James A Raleigh pimonidazole antibody Jonathan Coles L.A. and C.L. were supported by grants from the Austrian Science Fund (FWF P18840, P19585, and P21533) and the Vienna Science and Technology Fund (WWTF LS0535). We are grateful for gifts of antibodies from Masayoshi Maeshima (anti-PIP2;1 and anti-γTIP1;1), Jürgen Denecke (anti-BiP), David G. Robinson (anti-SEC12), Lukas Mach (anti-MannII), Jiří Friml (anti-PIN1), and Shuh-ichi Nishikawa (anti-ERdj3A and anti-ERdj3B). Mutant Arabidopsis thaliana lines were kindly provided by Richard Strasser (hgl1-1), Shuh-ichi Nishikawa (erdj3a-1 and erdj3b-1), and Amir Sherman (pin1). L.A. thanks Lukas Mach for sharing reagents and advice and for critical reading of the manuscript. We also thank the anonymous reviewers for their insightful and helpful comments. antibodies Masayoshi Maeshima anti-PIP2;1 anti-γTIP1;1 Jürgen Denecke anti-BiP David G. Robinson anti-SEC12 Lukas Mach anti-MannII Jiří Friml anti-PIN1 Shuh-ichi Nishikawa anti-ERdj3A anti-ERdj3B Arabidopsis thaliana lines Richard Strasser hgl1-1 Shuh-ichi Nishikawa erdj3a-1 erdj3b-1 Amir Sherman pin1 Lukas Mach L.A. C.L. Austrian Science Fund Vienna Science and Technology antibodies Masayoshi Maeshima anti-PIP2;1 anti-γTIP1;1 Jürgen Denecke anti-BiP David G. Robinson anti-SEC12 Lukas Mach anti-MannII Jiří Friml anti-PIN1 Shuh-ichi Nishikawa anti-ERdj3A anti-ERdj3B Arabidopsis thaliana Mutant Arabidopsis thaliana lines Richard Strasser hgl1-1 Shuh-ichi Nishikawa erdj3a-1 erdj3b-1 Amir Sherman pin1 Lukas Mach L.A. C.L. Austrian Science Fund Vienna Science and Technology antibodies Masayoshi Maeshima anti-PIP2;1 anti-γTIP1;1 Jürgen Denecke anti-BiP David G. Robinson anti-SEC12 Lukas Mach anti-MannII Jiří Friml anti-PIN1 Shuh-ichi Nishikawa anti-ERdj3A anti-ERdj3B Mutant Arabidopsis Richard Strasser hgl1-1 Shuh-ichi Nishikawa erdj3a-1 erdj3b-1 Amir Sherman L.A. pin1 Lukas Mach We appreciate the invaluable contributions of the nephrology community who provide information to, and maintain, the ANZDATA and Stoke-on-Trent databases; this includes physicians, surgeons, nurses, renal technicians, database managers, and most importantly, the patients. We are especially grateful to all patients and healthy volunteers in Cardiff for participating in the immunological study, and the staff on the B5 ward for their cooperation. We also thank Hassan Jomaa for providing synthetic HMB-PP and HMB-PCP; Ruggero Pardi for anti-CD11a blocking antibodies; Ann Kift-Morgan for technical assistance; and Eric Champagne, Massimo Massaia, Eric Oldfield, Chiara Riganti and Tim Walsh for the stimulating discussion. Cardiff Hassan Jomaa HMB-PP HMB-PCP Ruggero Pardi anti-CD11a antibodies Ann Kift-Morgan Eric Champagne Massimo Massaia Eric Oldfield Chiara Riganti Tim Walsh nephrology community ANZDATA Stoke-on-Trent Hassan Jomaa synthetic HMB-PP HMB-PCP Ruggero Pardi anti-CD11a blocking antibodies Ann Kift-Morgan Eric Champagne Massimo Massaia Eric Oldfield Chiara Riganti Tim Walsh We thank Gerhard Adam for yeast strains and vector pADHfw, Lukas Mach for providing the anti-HA antibody and Karin Polacsek (all BOKU-Vienna) for N-glycan analysis. We thank Dieter H. Wolf (University of Stuttgart, Stuttgart, Germany) for the kind gift of CPY* expression vector pRS306-prc1-1, Frans E. Tax (University of Arizona, Tucson, AZ) for the kind gift of bri1-9 seeds, Cyril Zipfel (The Sainsbury Laboratories, Norwich, UK) for efr-1 seeds and elf18 peptide and Ikuko Hara-Nishimura (Department of Botany, Graduate School of Science, Kyoto University, Kyoto, Japan) for the kind gift of anti-TGG1 antibodies. This work was supported by a grant from Austrian Science Fund (FWF): P20817-B12.Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. Gerhard Adam yeast strains vector pADHfw Lukas Mach anti-HA antibody Karin Polacsek BOKU-Vienna Dieter H. Wolf University of Stuttgart, Stuttgart, Germany CPY* expression vector pRS306-prc1-1 Frans E. Tax University of Arizona, Tucson, AZ bri1-9 seeds Cyril Zipfel The Sainsbury Laboratories, Norwich, UK efr-1 seeds elf18 peptide Ikuko Hara-Nishimura Department of Botany, Graduate School of Science, Kyoto University, Kyoto, Japan anti-TGG1 antibodies The authors acknowledge Rahul Warrior, Louise Parker and Jennifer Butler for critical reading of the manuscript. The Bloomington Drosophila Stock Center (Indiana University) and the Harvard Stock Center (Harvard University) provided Drosophila strains. We thank Pernille Rorth (IMCB, Singapore) for the tubulin-staiB2 and stainull lines and the pCaSpeR-tub vector, Lynne Cassimeris (Lehigh University, Bethlehem, PA) for the human STMN1 clone, Bill Saxton (UC Santa Cruz) for the Khc20 strain, Angels Almenar-Queralt and Alison Fisher for assistance with qRT-PCR and data analysis, and Coreina Teunissen for technical assistance with immunostaining. The monoclonal antibodies 22c10 and DCSP-2 6D6 developed by the Benzer lab were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biology, Iowa City, IA 52242. Rahul Warrior Louise Parker Jennifer Butler Bloomington Drosophila Stock Center Indiana University Harvard Stock Center Harvard University Drosophila strains Pernille Rorth IMCB, Singapore tubulin-staiB2 stainull lines pCaSpeR-tub vector Lynne Cassimeris Lehigh University, Bethlehem, PA STMN1 clone Bill Saxton UC Santa Cruz Khc20 strain Angels Almenar-Queralt Alison Fisher Coreina Teunissen antibodies 22c10 DCSP-2 6D6 Developmental Studies Hybridoma Bank University of Iowa, Department of Biology, Iowa City, IA Rahul Warrior Louise Parker Jennifer Butler Bloomington Drosophila Stock Center Indiana University Harvard Stock Center Harvard University Drosophila strains Pernille Rorth IMCB, Singapore tubulin-staiB2 stainull lines pCaSpeR-tub vector Lynne Cassimeris Lehigh University, Bethlehem, PA human STMN1 clone Bill Saxton UC Santa Cruz Khc20 strain Angels Almenar-Queralt Alison Fisher Coreina Teunissen monoclonal antibodies 22c10 DCSP-2 6D6 Benzer lab Developmental Studies Hybridoma Bank University of Iowa, Department of Biology, Iowa City, IA Rahul Warrior Louise Parker Jennifer Butler Bloomington Drosophila Stock Center Indiana University Harvard Stock Center Harvard University Drosophila Pernille Rorth tubulin-staiB2 pCaSpeR-tub vector Lynne Cassimeris Lehigh University, Bethlehem, PA human Bill Saxton UC Santa Cruz Khc20 Angels Almenar-Queralt Alison Fisher Coreina Teunissen monoclonal antibodies 22c10 DCSP-2 6D6 Benzer lab Developmental Studies Hybridoma Bank NICHD University of Iowa, Department of Biology, Iowa City, IA We thank Susumu Hirabayashi and Jill Fink for helpful discussions. We also thank the Bloomington Drosophila Stock Center and the Vienna Drosophila RNAi Center for providing stocks. The monoclonal antibody against Pericardin developed by Danielle Gratecos and monoclonal antibody against Actin developed by Jim Jung-Ching Lin were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biology, Iowa City, Iowa. Susumu Hirabayashi Jill Fink Bloomington Drosophila Stock Center Vienna Drosophila RNAi Center stocks antibody Pericardin Danielle Gratecos antibody Actin Jim Jung-Ching Lin Developmental Studies Hybridoma Bank The University of Iowa, Department of Biology, Iowa City, Iowa Susumu Hirabayashi Jill Fink Bloomington Drosophila Stock Center Vienna Drosophila RNAi Center stocks monoclonal antibody Pericardin Danielle Gratecos monoclonal antibody Actin Jim Jung-Ching Lin Developmental Studies Hybridoma Bank NICHD The University of Iowa Department of Biology We thank Stefanie Bauer, Carina Kruip, and Barbara Yutzy for expert technical assistance, Roland Plesker and his team for providing monkey blood, Oreste Acuto for reading the manuscript, and Thomas Hanke for the generous gift of the antibody TGN1412 and helpful discussion. Stefanie Bauer Carina Kruip Barbara Yutzy Roland Plesker monkey blood Oreste Acuto Thomas Hanke antibody TGN1412 The authors would like to thank Drs. Caroline Damsky and David Strom for antibodies obtained via the Developmental Studies Hybridoma Bank under the auspices of the NICHD and maintained by The University of Iowa (Iowa City, IA). The spinning disc device was designed and manufactured by Jeremy Riley, Ryan Tam, Joe Shu, and the UCSD Campus Research Machine Shop. Caroline Damsky David Strom antibodies Developmental Studies Hybridoma Bank The University of Iowa (Iowa City, IA) Jeremy Riley Ryan Tam Joe Shu UCSD Campus Research Machine Shop Caroline Damsky David Strom antibodies Developmental Studies Hybridoma Bank NICHD The University of Iowa (Iowa City, IA) spinning disc device Jeremy Riley Ryan Tam Joe Shu UCSD Caroline Damsky David Strom antibodies Developmental Studies Hybridoma Bank NICHD The University of Iowa spinning disc device Jeremy Riley Ryan Tam Joe Shu UCSD We thank Carsten Janke for purified microtubules, David J. Chen for DNA-PK (P-S2016) antibodies, Bérangère Lombard of the Institut Curie mass spectrometry and proteomics platform and Fabrice Cordelières and Pauline Chabosseau from the imaging platform. Carsten Janke microtubules David J. Chen DNA-PK (P-S2016) antibodies Bérangère Lombard Institut Curie mass spectrometry and proteomics platform Fabrice Cordelières Pauline Chabosseau Carsten Janke David J. Chen DNA-PK (P-S2016) antibodies Bérangère Lombard Institut Curie Fabrice Cordelières Pauline Chabosseau We are grateful to Tom Jessell and Jane Dodd for providing the Gdf7Cre/+ mice, and to Jane Johnson and Trish Savage for providing the Math1GFP/GFP mice. We are also grateful to Michael German for providing the rabbit anti-Lmx1a antibody. We thank Ty Abel for expert review of medulloblastoma pathology. We acknowledge Sean Schaffer at the Vanderbilt University Cell Imaging Shared Resource for use of the confocal microscope. Tom Jessell Jane Dodd Gdf7Cre/+ mice Jane Johnson Trish Savage Math1GFP/GFP mice rabbit anti-Lmx1a antibody Ty Abel Sean Schaffer Vanderbilt University Cell Imaging Shared Resource The antibodies against human CYP2E1 and CYP3A4 were kindly provided by Dr. M. Ingelman-Sundberg. This work was supported by a grant from the Deutsche Krebshilfe (70-1881-B01) to AP and CB. The authors would like to thank Dr. G.E. Arteel for proofreading the manuscript. antibodies CYP2E1 CYP3A4 Dr. M. Ingelman-Sundberg Deutsche Krebshilfe Dr. G.E. Arteel antibodies human CYP2E1 CYP3A4 Dr. M. Ingelman-Sundberg Deutsche Krebshilfe AP CB Dr. G.E. Arteel antibodies CYP2E1 CYP3A4 Dr. M. Ingelman-Sundberg Deutsche Krebshilfe AP CB Dr. G.E. Arteel This work was supported in part by grants-in-aid for Scientific Research 23590929 from the Ministry of Education, Culture, Sports, Science and Technology, Japan. We are grateful to Dr Hiroshi Okamoto from Tohoku University Graduate School of Medicine, Sendai, Japan, for providing the anti-REG Iα antibody. Ministry of Education, Culture, Sports, Science and Technology, Japan Hiroshi Okamoto Tohoku University Graduate School of Medicine, Sendai, Japan anti-REG Iα antibody Ministry of Education, Culture, Sports, Science and Technology, Japan Hiroshi Okamoto Tohoku University Graduate School of Medicine, Sendai, Japan anti-REG Iα antibody We thank the NIH AIDS Reagents and Reference Program for providing H9 cells, anti-p24 antibody, and pHIVNL4-3 construct. Gratitude goes to Hongjian Li, Tianhong Zhou, and Paul Rider for technical assistance and excellent suggestions. H9 cells anti-p24 antibody pHIVNL4-3 Construct Hongjian Li Tianhong Zhou Paul Rider Ataxin-1 monoclonal antibodies were obtained from the UC/Davis/NINDS/NIMH NeuroMab Facility. We thank Martina Callaghan for assistance with MATLAB and statistical analyses, and Rosella Abeti, Patrick Lewis and John Hardy for critical reading of this manuscript. Ataxin-1 monoclonal antibodies UC/Davis NINDS NIMH NeuroMab Facility Martina Callaghan Rosella Abeti Patrick Lewis John Hardy Ataxin-1 monoclonal antibodies UC/Davis NINDS NIMH NeuroMab Facility Martina Callaghan Rosella Abeti Patrick Lewis John Hardy The authors are grateful to G. Hime for providing Drosophila stocks and P. Gleeson for providing antibodies used in this work. G. Hime Drosophila stocks P. Gleeson antibodies G. Hime Drosophila P. Gleeson antibodies We are thankful to Prof. Dr. P. Macheroux (Institute of Biochemistry, Graz University of Technology, Austria) for providing us the Neurospora crassa CS antibody. We thank Dr. Sven Krappmann for helpful discussions. This work was supported by DFG research group grant FOR546.Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. Prof. Dr. P. Macheroux Institute of Biochemistry, Graz University of Technology, Austria Neurospora crassa CS antibody Dr. Sven Krappmann DFG research group Creative Commons Attribution Noncommercial License Prof. Dr. P. Macheroux Institute of Biochemistry, Graz University of Technology, Austria Neurospora crassa CS antibody Dr. Sven Krappmann DFG research group We acknowledge Natalia Shpiro for synthesis of GDC-0941, the antibody purification teams (Division of Signal Transduction Therapy, University of Dundee) co-ordinated by Hilary McLauchlan and James Hastie for generation of antibodies. We thank the Tayside Tissue Bank for immunohistochemistry. We also thank the Medical Research Council, the Wellcome Trust (SLD) and the pharmaceutical companies supporting the Division of Signal Transduction Therapy Unit (AstraZeneca, Boehringer-Ingelheim, GlaxoSmithKline, Merck KgaA and Pfizer) for financial support. Natalia Shpiro GDC-0941 antibody Division of Signal Transduction Therapy, University of Dundee Hilary McLauchlan James Hastie antibodies Tayside Tissue Bank Medical Research Council Wellcome Trust (SLD) Division of Signal Transduction Therapy Unit AstraZeneca, Boehringer-Ingelheim, GlaxoSmithKline Merck KgaA Pfizer Natalia Shpiro Division of Signal Transduction Therapy, University of Dundee Hilary McLauchlan James Hastie antibodies Tayside Tissue Bank Medical Research Council Wellcome Trust SLD Division of Signal Transduction Therapy Unit AstraZeneca Boehringer-Ingelheim GlaxoSmithKline Merck KgaA Pfizer The authors would like to thank Prof Brunangelo FALINI at the University of Perugia for kindly providing the NPM-mutant specific antibody. The authors would also like to thank MD MINDEN (Ontario Cancer Institute, Toronto, ON, Canada) for providing the OCI-AML3 cells. This work was supported by grants from the National Natural Science Foundation of China (No 81070429, 81372541 and 81060048). Prof Brunangelo FALINI University of Perugia NPM-mutant antibody MD MINDEN Ontario Cancer Institute, Toronto, ON, Canada OCI-AML3 cells National Natural Science Foundation of China Prof Brunangelo FALINI University of Perugia NPM-mutant specific antibody MD MINDEN OCI-AML3 National Natural Science Foundation of China We acknowledge Natalia Shpiro for synthesis of GDC-0941, the antibody purification teams (Division of Signal Transduction Therapy, University of Dundee) co-ordinated by Hilary McLauchlan and James Hastie for generation of antibodies. We thank the Tayside Tissue Bank for immunohistochemistry. We also thank the Medical Research Council, the Wellcome Trust (SLD) and the pharmaceutical companies supporting the Division of Signal Transduction Therapy Unit (AstraZeneca, Boehringer-Ingelheim, GlaxoSmithKline, Merck KgaA and Pfizer) for financial support. PNatalia Shpiro GDC-0941 antibody Division of Signal Transduction Therapy, University of Dundee Hilary McLauchlan James Hastie antibodies Tayside Tissue Bank Medical Research Council Wellcome Trust (SLD) Division of Signal Transduction Therapy Unit AstraZeneca, Boehringer-Ingelheim, GlaxoSmithKline Merck KgaA Pfizer We acknowledge Natalia Shpiro for synthesis of GDC-0941, the antibody purification teams (Division of Signal Transduction Therapy, University of Dundee) co-ordinated by Hilary McLauchlan and James Hastie for generation of antibodies. We thank the Tayside Tissue Bank for immunohistochemistry. We also thank the Medical Research Council, the Wellcome Trust (SLD) and the pharmaceutical companies supporting the Division of Signal Transduction Therapy Unit (AstraZeneca, Boehringer-Ingelheim, GlaxoSmithKline, Merck KgaA and Pfizer) for financial support. PNatalia Shpiro GDC-0941 antibody Division of Signal Transduction Therapy, University of Dundee Hilary McLauchlan James Hastie antibodies Tayside Tissue Bank Medical Research Council Wellcome Trust (SLD) Division of Signal Transduction Therapy Unit AstraZeneca, Boehringer-Ingelheim, GlaxoSmithKline Merck KgaA Pfizer We thank Julie Johnston (Penn Vector Core, University of Pennsylvania, PA, USA) for supplying the recombinant AAV vector pE5/9, Evelyn Bendrat and Michaela Miehe (UKE-HEXT, Hamburg, Germany) for AAV6 production. We thank Siegfried Labeit (Heidelberg, Germany) for providing the anti-titin Z1 antibody and Sakthivel Sadayappan (Loyola University Chicago, IL, USA) for providing antibodies directed against cMyBP-C-2-14aa, Ser273-, and Ser-302-cMyBP-C. We thank Vincent Christoffels and Malou van den Boogaard (Amsterdam, The Netherlands) for providing embryonic mouse heart samples. We also thank Cyriaque Beley (Inserm U974, Paris, France) for supplying the pAAV-U7 plasmid, as well as June Uebeler (UKE-Pharmacology, Hamburg, Germany) for technical help. This work was supported by the Deutsche Forschungsgemeinschaft (FOR-604/1-2, CA 618/1-2), the Fritz Thyssen Stiftung (Az. 10.09.1.139), the seventh Framework Program of the European Union (Health-F2-2009-241577; Big-Heart project), and by the German Centre for Cardiovascular Research (Deutsches Zentrum für Herz-Kreislauf-Forschung e.v., DZHK).Supporting Information is available at EMBO Molecular Medicine Online.The authors declare that they have no conflict of interest. Julie Johnston Penn Vector Core, University of Pennsylvania recombinant AAV vector pE5/9 Evelyn Bendrat Michaela Miehe UKE-HEXT, Hamburg, Germany AAV6 Siegfried Labeit anti-titin Z1 antibody Sakthivel Sadayappan Loyola University Chicago, IL, USA antibodies cMyBP-C-2-14aa Ser273 Ser-302 cMyBP-C Vincent Christoffels Malou van den Boogaard embryonic mouse heart Cyriaque Beley Inserm U974 pAAV-U7 plasmid June Uebeler UKE-Pharmacology Deutsche Forschungsgemeinschaft Fritz Thyssen Stiftung German Centre for Cardiovascular Research EMBO Molecular Medicine Online Julie Johnston Penn Vector Core, University of Pennsylvania recombinant AAV vector pE5/9 Evelyn Bendrat Michaela Miehe UKE-HEXT, Hamburg, Germany AAV6 Siegfried Labeit Heidelberg, Germany anti-titin Z1 antibody Sakthivel Sadayappan Loyola University Chicago, IL, USA antibodies cMyBP-C-2-14aa Ser273 Ser-302 cMyBP-C Vincent Christoffels Malou van den Boogaard Amsterdam, The Netherlands mouse Cyriaque Beley Inserm U974, Paris, France pAAV-U7 plasmid June Uebeler UKE-Pharmacology, Hamburg, Germany Deutsche Forschungsgemeinschaft Fritz Thyssen Stiftung European Union German Centre for Cardiovascular Research EMBO The authors thank Dr. P. Ehrenberg for providing CM235 plasmid DNA, Dr. J. Sodroski for providing 517B plasmid DNA, and Dr. T. Hope for providing the GFP-Vpr vector. Ms. I. M. Totillo for manuscript preparation. The following reagents were provided through the AIDS Research and Reference Reagent Program, DAIDS, NIAID, NIH: pLai from Dr. K. Peden, pNL4-3 from Dr. M. Martin, anti-p24 monoclonal antibody from Dr. B. Chesebro and Dr. K. Wehrly, and recombinant soluble CD4 from Progenics Pharmaceuticals, Inc. This work was supported by grants NS31492, DA017618, NS054580, and NS39191 from the National Institutes of Health, Public Health Service. Dr. P. Ehrenberg CM235 plasmid Dr. J. Sodroski 517B plasmid Dr. T. Hope GFP-Vpr vector Ms. I. M. Totillo AIDS Research and Reference Reagent Program, DAIDS, NIAID, NIH pLai Dr. K. Peden pNL4-3 Dr. M. Martin anti-p24 monoclonal antibody Dr. B. Chesebro Dr. K. Wehrly recombinant soluble CD4 Progenics Pharmaceuticals, Inc National Institutes of Health Public Health Service We thank Dr. Ken Mann and Dr. Tom Orfeo from the Department of Biochemistry at the University of Vermont for providing antibodies to factor V, Dr. Michael Nesheim and Dr. Paul Kim from the Department of Biochemistry at Queen’s University for providing cells expressing rMZ-II and rP2-II, and Dr. Kathleen Berkner from the Department of Molecular Cardiology at The Cleveland Clinic for helpful advice. We thank Dr. Susan Kennedy-Kalafatis and Dr. Ed Plow for helpful advice and for critical reading of the manuscript. Dr. Ken Mann Dr. Tom Orfeo Department of Biochemistry University of Vermont antibodies factor V Dr. Michael Nesheim Dr. Paul Kim Department of Biochemistry at Queen’s University rMZ-II rP2-II Dr. Kathleen Berkner Department of Molecular Cardiology Cleveland Clinic Dr. Susan Kennedy-Kalafatis Dr. Ed Plow We acknowledge the C3M imaging Core Facility (MICA) facility. We thank B. Dreno, M. Herlyn and R. Halaban for melanoma cells, A. Cano and E.R. Fearon for plasmids and A. Garcia de Herreros for anti-SNAIL antibodies. We also thank P. Savagner and D. Bennett for helpful discussions. We are grateful to the BIORDERM INSERM network for their contribution. C3M imaging Core Facility B. Dreno M. Herlyn R. Halaban melanoma A. Cano E.R. Fearon plasmids A. Garcia de Herreros anti-SNAIL P. Savagner D. Bennett BIORDERM INSERM The authors would like to thank their colleagues at the Wellcome Trust Centre for Cell Biology, University of Edinburgh, and at the Centre National de la Recherche Scientifique, UMR 2587, Toulouse, for stimulating discussions and technical help. Antibody against BubR1 was kindly provided by Dr Tim Yen, Fox Chase Center, Philadelphia, PA. The work was supported by a Wellcome Trust Senior Research Fellowship to A.M., and in part by salary support from the French 'Centre National de la Recherche Scientifique'. Wellcome Trust Centre University of Edinburgh Centre National de la Recherche Scientifique, UMR 2587, Toulouse Antibody BubR1 Dr Tim Yen Fox Chase Center, Philadelphia, PA Wellcome Trust A.M. French 'Centre National de la Recherche Scientifique' We acknowledge Prof. Jonathan M. G. Higgins, Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America for kindly gifting us E-Cadherin antibody. Prof. Jonathan M. G. Higgins Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston E-Cadherin Antibody We thank members of the Wallrath laboratory for comments regarding the manuscript and R. Imani, M. Frauenholtz and L. Rydberg for technical assistance. We are thankful for the kind gift of Bocksbeutel antibodies from N. Wagner and G. Krohne. Wallrath laboratory R. Imani M. Frauenholtz L. Rydberg Bocksbeutel antibodies N. Wagner G. Krohne We thank G. Meissner for the generous gift of anti-RyR2 antibody C3-33 and Y. Chen for providing the photomicrograph used in Figure 2. G. Meissner anti-RyR2 antibody C3-33 Y. Chen We are grateful to Adriana Villella, John Rieffel, and Jeff Hall (Brandeis University) for generously providing, and assisting in the use of, LifeSongX software. We thank Gyunghee Lee for the generous gift of Dsx antibody, Barry Dickson for fruΔtra and fruM flies, and Paul Schedl for dsx1 flies. We thank Megan Neville, Tony Dornan, Jeff Hall, and Michelle Arbeitman for comments on the manuscript. E.J.R., J.-C.B., and S.F.G. were supported by grants from the Wellcome Trust. Adriana Villella John Rieffel Jeff Hall Brandeis University Gyunghee Lee Dsx antibody Barry Dickson Paul Schedl Megan Neville Tony Dornan Michelle Arbeitman V. We thank Prof. K. Inoue (Saitama University, Japan) for supplying the transgenic rats, Prof. K. Wakabayashi (Gunma University, Japan) for providing the anti-rat GH and rat PRL antibodies, Prof. T Nakamura (Hokkaido University, Japan) for supplying type I and type III collagen antibodies, and David Kipler, ELS (Supernatant Communications) for revising the language of the manuscript. This work was partly supported by a Grant-in-Aid for Scientific Research (C) (22590192) and by a Grant-in-Aid for Young Scientists (B) (23790233) (25860147) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, by promotional funds for the Keirin Race of the Japan Keirin Association, and by the Jichi Medical University Young Investigator Award from Jichi Medical University School of Medicine. K. Inoue Saitama University K. Wakabayashi Gunma University anti-rat GH rat PRL antibodies T Nakamura Hokkaido University collagen antibodies David Kipler Japan Keirin Association Jichi Medical University School of Medicine No potential conflicts of interest relevant to this article were reported.É.É. performed ELISpot assays with human PBLs. R.K. and E.B. identified candidate ZnT8 epitopes. J.-B.A., J.B., and J.-J.R. recruited patients and obtained ethical approval. Y.H. and C.M. purified and HLA-typed patient lymphocytes. B.M. and L.C. performed autoantibody testing and helped with patient recruitment. P.v.E. designed and supervised the study and wrote the manuscript. P.v.E. is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.The authors are grateful to Drs. Caroline Mignot (Ambroise Paré Hospital), Vincent Gajdos (Antoine Béclère Hospital), Sophie Lemerle (Intercommunal Hospital Créteil), and Agnes Hartemann (Pitié-Salpétrière Hospital) for patient recruitment. É.É. R.K. E.B. J.-B.A. J.B. J.-J.R. Y.H. C.M. B.M. L.C. collagen antibodies David Kipler Japan Keirin Association Jichi Medical University School of Medicine We thank Dr. Gabriela Arroyo for technical help and Dr. Ron Geller for comments on the manuscript. We also thank members of the Frydman lab and Dr. Tomas Aragon for valuable advice, technical help, and discussions and Brown lab for technical help on microarrays protocols. We also thank Drs. Peter Walter, Mark Rose, Jonathan Warner, and Martin Pool for kindly providing yeast strains, reagents, and antibodies. Dr. Gabriela Arroyo Dr. Ron Geller Frydman lab Dr. Tomas Aragon Brown lab Peter Walter Mark Rose Jonathan Warner Martin Pool yeast strains antibodies The authors thank Michael Boyles and David Wilson for their invaluable expertise in image processing (CIRT; Center for Instruction and Research Technology, University of North Florida). The monoclonal antibody C17 created by J.R. Sanes, was obtained from the Developmental Studies Hybridoma Bank, created by the National Institute of Child Health and Human Development of the National Institutes of Health and maintained at The University of Iowa, Department of Biology, Iowa City, IA, USA 52242. Michael Boyles David Wilson University of North Florida monoclonal antibody C17 J.R. Sanes Developmental Studies Hybridoma Bank National Institute of Child Health National Institutes of Health he University of Iowa This study was generously sponsored by Novartis Animal Health, St. Aubin, Switzerland. We are grateful to Peter Moore for providing the CD1c antibody, to Marcus Doherr for statistical advice and to Vivianne Molitor and Viviane Neuhaus for technical help. Novartis Animal Health Peter Moore CD1c antibody Marcus Doherr Vivianne Molitor Viviane Neuhaus We would like to thank Ms. Rachel M. Faber for animal husbandry, Wai Pang Chan for assistance with confocal microscopy and Bobbie Schnieder for assistance with electron microscopy. The monoclonal antibodies to label skeletal muscle fiber-types were purchased from the Developmental Studies Hybridoma Bank at the University of Iowa. Ms. Rachel M. Faber Wai Pang Chan Bobbie Schnieder monoclonal antibodies Developmental Studies Hybridoma Bank University of Iowa We are grateful to J. L. Mandel, M. Frontini, T. Hussenet, and S. DuManoir for helpful discussions and advice with ChIP and FISH experiments, and to F. Winston for critically reading the manuscript. We thank R. Losson, X. Zhu, C. Craft, D. Hicks, and G. Manfioletti for providing antibodies. We also thank F. Landmann, C. Weber, and staff from the Institut Clinique de la Souris for their contribution to this work.Funding. This work was supported by funds from Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS), Hôpitaux Universitaires de Strasbourg, and Collège de France; the Fonds National de la Science ACI, European Community RTN (HPRN-CT-2004–504228), STREP (LSHG-CT-2004–502950), and AICR (03–084) grants (to LT); and the National Organization for Rare Disorders (NORD) and European Community (EUROSCA integrated project, LSHM-CT-2004–503304) grant (to DD and YT).Competing interests. The authors have declared that no competing interests exist. J. L. Mandel M. Frontini T. Hussenet S. DuManoir F. Winston R. Losson C. Craft D. Hicks G. Manfioletti antibodies F. Landmann C. Weber Institut Clinique de la Souris Institut National de la Santé Centre National de la Recherche Scientifique Hôpitaux Universitaires de Strasbourg Collège de France Fonds National de la Science ACI National Organization for Rare Disorders We are grateful to Dr. Angus Nairn for the DARPP-32 antibody. Dr. Angus Nairn DARPP-32 antibody This study was funded by the Danish Medical Research Council for Independent Research and the Novo nordisk Foundation. We would also like to thank Prof. Graham Hardy (Dundee University, Dundee, U.K) for providing the PDH phospho specific, PDK4 and PDP1 antibodies. Centre of Inflammation and Metabolism, (CIM) is supported by a grant from the Danish National Research Foundation (# 02-512-55). Danish Medical Research Council Novo nordisk Foundation Prof. Graham Hardy Dundee University PDH phospho specific PDK4 PDP1 Centre of Inflammation and Metabolism Danish National Research Foundation We thank Richard Losick for critical reading of the manuscript, Adam Driks for providing the anti-TasA antibody, Saad Fakhry for his contribution to the mutagenesis experiments and Luciano Di Iorio for technical assistance. Richard Losick Adam Driks anti-TasA antibody Saad Fakhry Luciano Di Iorio NCG thanks Walter J. Gehring for his generous support to initiate and carry out the presented work on the function of γCOP in the embryo in his laboratory and Marc Neumann for his initial investigation of γCOP mutant embryos. NCG thanks Jennifer Lippincott-Schwartz for the possibility to complete this manuscript while working in her laboratory. SL and MA thank Christos Samakovlis for exchange of unpublished information. SL thanks Christian Lehner for support and discussions. We thank Kristina Armbruster for critical reading of the manuscript. We thank the Bloomington stock center for sending us numerous fly stocks. We thank Lydia Michaut and Makiko Seimyia for sharing their experience with DIG Southerns with us. We thank Makiko Seimyia for antibody stainings. We thank Konrad Basler and R. Tsien for plasmids. We thank Konrad Basler, Bruno Bello, Bill Engels, Roger Karress, Urs Kloter and Ulrich Schäfer for fly stocks. We are grateful to Karin Mauro, Gina Evora and Bernadette Bruno for providing us with excellent fly food. J. Gehring Marc Neumann Jennifer Lippincott-Schwartz Christos Samakovlis Christian Lehner Kristina Armbruster Bloomington stock center fly stocks Lydia Michaut Makiko Seimyia antibody stainings Konrad Basler R. Tsien plasmids Bruno Bello Bill Engels Roger Karress Urs Kloter Ulrich Schäfer fly stocks Karin Mauro Gina Evora Bernadette Bruno We gratefully acknowledge Joseph Tsien and Zhenzhong Cui (Boston University), Michael Brenner (University of Alabama, Birmingham), Robin Reed (Harvard Medical School), and Gideon Dreyfuss (University of Pennsylvania) for generous gifts of plasmid and antibody reagents. We are indebted to Jon Johnson (University of Pittsburgh) for advice on the use of receptor antagonists and on the growth and manipulation of primary cortical cultures. We thank Jon Johnson and members of the Grabowski lab for helpful discussions and a critical reading of the manuscript. Joseph Tsien Zhenzhong Cui Boston University Michael Brenner University of Alabama Robin Reed Harvard Medical School Gideon Dreyfuss University of Pennsylvania plasmid antibody Jon Johnson University of Pittsburgh Grabowski lab We thank Bloomington Drosophila Stock Center at Indiana University for fly stocks and Developmental Hybridoma Bank at Iowa University for antibodies. This work was supported by the Agenzia di Ricerca per la Sclerosi Laterale Amiotrofica (AriSLA). Bloomington Drosophila Stock Center Indiana University fly stocks Developmental Hybridoma Bank Iowa University antibodies Agenzia di Ricerca per la Sclerosi Laterale Amiotrofica We would like to express our thanks to Masayoshi Mishina for providing the OMP:tauDsRed construct, Stefan Schulte-Merke for the Rag1 mutant line, Shuo Lin for the Rag1:GFP line and the Qian Hu Fish Co. for help with fish transport. The SV2 antibody developed by Kathleen Buckley was obtained from the Developmental Studies Hybridoma Bank. This work was supported by the Temasek Life Sciences Laboratory, Singapore Millenium Foundation, Max-Planck Society, DFG (SFB 488) and the Boehringer Ingelheim Fonds. Masayoshi Mishina OMP:tauDsRed construct Stefan Schulte-Merke Rag1 mutant line Shuo Lin Rag1:GFP line Qian Hu Fish Co SV2 antibody Kathleen Buckley Developmental Studies Hybridoma Bank emasek Life Sciences Laboratory Singapore Millenium Foundation Max-Planck Society Boehringer Ingelheim Fonds We thank Chris Lundquist for purification of primary T cells and Toshiaki Kodama for the full-length SIVmac239 proviral construct. The following reagents were obtained through the NIH AIDS Research and Reference Reagent Program: HIV-1 p24 monoclonal antibody from Dr. Bruce Chesebro, and SIV p27 monoclonal antibody from Dr. Niels Pedersen. Chris Lundquist T cells Toshiaki Kodama SIVmac239 proviral construct NIH HIV-1 p24 monoclonal antibody Dr. Bruce Chesebro SIV p27 monoclonal antibody Dr. Niels Pedersen We thank Chris Lundquist for purification of primary T cells and Toshiaki Kodama for the full-length SIVmac239 proviral construct. The following reagents were obtained through the NIH AIDS Research and Reference Reagent Program: HIV-1 p24 monoclonal antibody from Dr. Bruce Chesebro, and SIV p27 monoclonal antibody from Dr. Niels Pedersen. Chris Lundquist T cells Toshiaki Kodama SIVmac239 proviral construct NIH HIV-1 p24 monoclonal antibody Dr. Bruce Chesebro SIV p27 monoclonal antibody Dr. Niels Pedersen