Version 1
: Received: 15 January 2018 / Approved: 16 January 2018 / Online: 16 January 2018 (06:38:11 CET)
How to cite:
Huang, H.-C.; Lee, M.-S.; Chang, W.-T.; Chen, H.-Y.; Chen, Y.-H.; Lin, C.-C.; Lin, M.-K. Three Bufadienolides-Induced Human Lung Cancer CL1-5 Cell Death Mainly through Autophagy. Preprints2018, 2018010134. https://doi.org/10.20944/preprints201801.0134.v1
Huang, H.-C.; Lee, M.-S.; Chang, W.-T.; Chen, H.-Y.; Chen, Y.-H.; Lin, C.-C.; Lin, M.-K. Three Bufadienolides-Induced Human Lung Cancer CL1-5 Cell Death Mainly through Autophagy. Preprints 2018, 2018010134. https://doi.org/10.20944/preprints201801.0134.v1
Huang, H.-C.; Lee, M.-S.; Chang, W.-T.; Chen, H.-Y.; Chen, Y.-H.; Lin, C.-C.; Lin, M.-K. Three Bufadienolides-Induced Human Lung Cancer CL1-5 Cell Death Mainly through Autophagy. Preprints2018, 2018010134. https://doi.org/10.20944/preprints201801.0134.v1
APA Style
Huang, H. C., Lee, M. S., Chang, W. T., Chen, H. Y., Chen, Y. H., Lin, C. C., & Lin, M. K. (2018). Three Bufadienolides-Induced Human Lung Cancer CL1-5 Cell Death Mainly through Autophagy. Preprints. https://doi.org/10.20944/preprints201801.0134.v1
Chicago/Turabian Style
Huang, H., Chi-Chen Lin and Ming-Kuem Lin. 2018 "Three Bufadienolides-Induced Human Lung Cancer CL1-5 Cell Death Mainly through Autophagy" Preprints. https://doi.org/10.20944/preprints201801.0134.v1
Abstract
Lung cancer is almost the most common cause of cancer death in the world. Clinically, the conventional therapy to eradicate the cancer cells is chemotherapy but a better drug remains required. In this study, the effects of three bufadienolides, kalantuboside B, kalantuboside A and bryotoxin C, isolated from Kalanchoe tubiflora (Harvey) were evaluated and characterized in CL1-5 highly metastatic human lung cancer cells. Contrary to the apoptosis-promoting activity in other cancer cells, these three bufadienolides did not induce apoptosis in CL1-5 cancer cells. Instead, they activated an autophagy pathway, as indicated by the increase of autophagosomes formation. The induction of autophagy by these three bufadienolides was demonstrated to link to down-regulation of p-mTOR as well as up-regulation of LC3-II, ATG5, ATG7, Beclin-1. Moreover, among these three compounds, kalantuboside B in which a monosaccharide is attached at the bufadienolide aglycon, exhibited a better autophagy induction. Our findings revealed an alternative mechanism of drug action by bufadienolides in lung cancer cells and provided evidence for the possibility of treating highly metastatic human lung cancer through an autophagy pathway.
Keywords
Kalanchoe tubiflora; bufadienolide; CL1-5 human lung cancer cells; autophagy
Subject
Medicine and Pharmacology, Pharmacology and Toxicology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.