Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Effects of Synergistic Inhibition on α-Glucosidase by Phytoalexins in Soybeans

Version 1 : Received: 18 October 2019 / Approved: 19 October 2019 / Online: 19 October 2019 (02:20:45 CEST)

A peer-reviewed article of this Preprint also exists.

Son, H.-U.; Yoon, E.-K.; Yoo, C.-Y.; Park, C.-H.; Bae, M.-A.; Kim, T.-H.; Lee, C.H.; Lee, K.W.; Seo, H.; Kim, K.-J.; Lee, S.-H. Effects of Synergistic Inhibition on α-glucosidase by Phytoalexins in Soybeans. Biomolecules 2019, 9, 828. Son, H.-U.; Yoon, E.-K.; Yoo, C.-Y.; Park, C.-H.; Bae, M.-A.; Kim, T.-H.; Lee, C.H.; Lee, K.W.; Seo, H.; Kim, K.-J.; Lee, S.-H. Effects of Synergistic Inhibition on α-glucosidase by Phytoalexins in Soybeans. Biomolecules 2019, 9, 828.

Abstract

To determine the mode of action of the effects of phytoalexins in soybeans, we analyzed enzyme inhibition kinetics using Michaelis–Menten plots and the Lineweaver–Burk plots. The results showed that glyceollin showed competitive inhibition, genistein showed noncompetitive, daidzein was uncompetitive, and luteolin showed a mixed mode of action. The Ki values were determined using a Dixon plot as: glyceollin, 18.99; genistein, 15.42; luteolin, 16.81; and daidzein, 9.99 μM, respectively. Furthermore, potential synergistic effects between glyceollin and the three designated polyphenols were investigated. A combination of glyceollin and luteolin (the ratio of 3:7 of glyceollin and luteolin) had synergistic effects on α-glucosidase inhibition according to combination index (CI)-isobologram equation. Collectively, these results showed that a combination of glyceollin and luteolin has the potential to inhibit α-glucosidase activity via a synergistic mode of action.

Keywords

α-glucosidase; glyceollin; genistein; luteolin; daidzein; phytoalexins; enzyme kinetics; combination index

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

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