Eichornia crassipes is one of the most dangerous aquatic weeds for Lake Tana and other water Bodies of Ethiopia .Use of natural enemies of the weed to discourage its propagation and impact is one of the best recommended options by scientific society.Among them, there are more fungi naturally a pathogen for water hyacinth and other plants. To use those pathogens to manage the highly water hyacinth infestation area of Lake Tana specfically Gondar Zuria woreda , Lemeba Kebela , infected water hyacinth plant material by fungi were collected from Goregora side of Lake Tana. The collection was done from infected water hyacinth leaves by fungi.All isolated fungi(Zygomycota .,Candidaalbicanaa,Trichoderma ,Aspergillus flavus, Fusarium s,Aspergillus niger,Pencillium and Rhizocotosolani spp) were attempted to infect the healthy water hyacinth in Lemeba Kebela by spraying 2ooo ml cultured mixed fungi in 20 metre square areas and after three weeks of exprement the fungi were severely affected the weeds and expanded in all wetland areas of lemba kebala that infested by water hyacinth but in some exprement areas the leaf of water hyacinth show disease symptoms after 7 days of fungi releasd on targeted area.This observational research shows that the fungi were high potential to attack healthy water hyacinth at high enviroment temprature and at less humidity