Study evaluates the characteristics and clinical course of patients with iris cysts in the long term of follow-up (24-48 months). We analyzed retrospectively the medical records of 39 patients with iris cysts (27 female and 12 male). Age, visual acuity, intraocular pressure (IOP), slit-lamp evaluation, and ultrasound biomicroscopy images were assessed. The mean age at diagnosis was 40.6±17.48 years. 30 (76.9%) cysts were peripheral, 5 (12.8%) cysts located at the pupillary margin, 2 (5.1%) cysts were midzonal and 2 (5.1%) multichamber cysts extended from the periphery to the pupillary margin. 23 (59%) cysts were in the lower temporal quadrant, 11 (28.2%) cysts in the lower nasal quadrant, and 5 (12.8%) cysts in the upper nasal quadrant. Cyst size was positively correlated with the age of the patients (rs = 0.38, p = 0.003) and negatively correlated with visual acuity (rs = -0.42, p = 0.014). No cyst growth was documented. The only complication was an increase in IOP in 3 (7.7%) patients with multiple cysts. The anatomical location of cysts cannot differentiate them from solid tumors. The vast majority of cysts are asymptomatic, do not increase in size, and do not require treatment in long-term follow-up.