Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Effects of Aqueous Dispersions of C60, C70 and Gd@C82 Fullerenes on Genes Involved in Oxidative Stress and Anti-inflammatory Pathways

Elena V. Proskurnina
* ORCID logo ,
Ivan V. Mikheev
ORCID logo ,
Ekaterina A. Savinova
,
Elizaveta S. Ershova
,
Natalia N. Veiko
,
Larisa V. Kameneva
,
Olga A. Dolgikh
ORCID logo ,
Ivan V. Rodionov
,
Mikhail A. Proskurnin
ORCID logo ,
and Svetlana V. Kostyuk
Version 1 : Received: 3 May 2021 / Approved: 7 May 2021 / Online: 7 May 2021 (09:38:38 CEST)

A peer-reviewed article of this Preprint also exists.

Proskurnina, E.V.; Mikheev, I.V.; Savinova, E.A.; Ershova, E.S.; Veiko, N.N.; Kameneva, L.V.; Dolgikh, O.A.; Rodionov, I.V.; Proskurnin, M.A.; Kostyuk, S.V. Effects of Aqueous Dispersions of C60, C70 and Gd@C82 Fullerenes on Genes Involved in Oxidative Stress and Anti-Inflammatory Pathways. Int. J. Mol. Sci. 2021, 22, 6130. Proskurnina, E.V.; Mikheev, I.V.; Savinova, E.A.; Ershova, E.S.; Veiko, N.N.; Kameneva, L.V.; Dolgikh, O.A.; Rodionov, I.V.; Proskurnin, M.A.; Kostyuk, S.V. Effects of Aqueous Dispersions of C60, C70 and Gd@C82 Fullerenes on Genes Involved in Oxidative Stress and Anti-Inflammatory Pathways. Int. J. Mol. Sci. 2021, 22, 6130.

Abstract

Background: Fullerenes and metallofullerenes can be considered promising nanopharmaceuticals themselves and as a basis for chemical modification. As reactive oxygen species homeostasis plays a vital role in cells, the study of their effect on genes involved in oxidative stress and anti-inflammatory response is of particular importance. Methods: Human fetal lung fibroblasts were incubated with aqueous dispersions of C60, C70, and Gd@C82 in concentrations of 5 nM and 1.5 µM for 1, 3, 24, and 72 hours. Cell viability, intracellular ROS, NOX4, NFκB, PRAR-γ, NRF2, heme oxygenase 1, and NAD(P)H quinone dehydrogenase 1 expression have been studied. Results & conclusion: The aqueous dispersions of C60, C70, and Gd@C82 fullerenes are active participants in ROS homeostasis. Low and high concentrations of AFDs have similar effects. C70 was the most inert substance, C60 was the most active substance. All AFDs have both a “prooxidant” and “antioxidant” effect, but with a different balance. Gd@C82 was a substance with more pronounced antioxidant and anti-inflammatory properties, while C70 had more pronounced “prooxidant” properties.

Keywords

aqueous fullerene dispersions; pristine fullerenes; metallofullerenes; ROS homeostasis; oxidative stress; NOX4; Nrf2; PRAR-γ; heme oxygenase 1; NAD(P)H quinone dehydrogenase 1; an-ti-inflammatory pathways

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.