Zagros is an important region with high quality and quantity karstic water resources in the Middle East. This region provides a dominant part of potable and agricultural water needs for its inhabitants as well as agricultural water needs for nearby regions. Therefore, studying karstic water resources in Zagros by accurate methods such as stable isotopes techniques is very important. In this investigation, hydrological characteristics of groundwater resources including groundwater origin, recharge rate and recharge elevation have been studied using stable isotopes (¹⁸O and ²H). The results show that stable isotopes signatures in groundwater resources show notable variations across Zagros and groundwater resource mainly plot on south and west Zagros meteoric water lines. In addition, recharge elevation and recharge rate in groundwater resources also show significant variations in Zagros. Finally, the stable isotopes signatures in precipitation and groundwater has been used to study the role of each dominant air mass (contribution percentage of precipitation events originate from each air mass) in groundwater resources recharge using Simmr package in R language. Overall, groundwater resources in Zagros is recharged by precipitation events originate from various air masses and they have various recharge rates and recharge elevations.