Version 1
: Received: 23 November 2022 / Approved: 25 November 2022 / Online: 25 November 2022 (02:52:37 CET)
How to cite:
Pires, M. C.; Andriolo, N. D. G.; Lopes, B. R. P.; Delarmelina, C.; Duarte, M. C. T.; Ruiz, A. L. T. G.; Do Nascimento, V. M. G.; De Toledo, K. A.; Dos Santos, C. New Insights into the Biological Activities of Carboxymethylated. Preprints2022, 2022110465. https://doi.org/10.20944/preprints202211.0465.v1
Pires, M. C.; Andriolo, N. D. G.; Lopes, B. R. P.; Delarmelina, C.; Duarte, M. C. T.; Ruiz, A. L. T. G.; Do Nascimento, V. M. G.; De Toledo, K. A.; Dos Santos, C. New Insights into the Biological Activities of Carboxymethylated. Preprints 2022, 2022110465. https://doi.org/10.20944/preprints202211.0465.v1
Pires, M. C.; Andriolo, N. D. G.; Lopes, B. R. P.; Delarmelina, C.; Duarte, M. C. T.; Ruiz, A. L. T. G.; Do Nascimento, V. M. G.; De Toledo, K. A.; Dos Santos, C. New Insights into the Biological Activities of Carboxymethylated. Preprints2022, 2022110465. https://doi.org/10.20944/preprints202211.0465.v1
APA Style
Pires, M. C., Andriolo, N. D. G., Lopes, B. R. P., Delarmelina, C., Duarte, M. C. T., Ruiz, A. L. T. G., Do Nascimento, V. M. G., De Toledo, K. A., & Dos Santos, C. (2022). New Insights into the Biological Activities of Carboxymethylated. Preprints. https://doi.org/10.20944/preprints202211.0465.v1
Chicago/Turabian Style
Pires, M. C., Karina Alves De Toledo and Catarina Dos Santos. 2022 "New Insights into the Biological Activities of Carboxymethylated" Preprints. https://doi.org/10.20944/preprints202211.0465.v1
Abstract
Lasiodiplodan, an exocellular Lasiodiplodia theobromae β-glucan exopolysaccharide (LaEPS), has at-tracted interest because of its antioxidant, antimicrobial, anti-inflammatory, and antiproliferative properties. LaEPS carboxymethylation enhances LaEPS water solubility and biological activities. However, carboxymethylated fractions of LaEPS (LaEPS-C): LLaEPS-C (Linear) and BLaEPS-C (Branched) were not widely studied yet. So, LaEPS-C, LLaEPS-C, and BLaEPS-C were assessed for their antioxidant, antimicrobial, antiproliferative, anticoagulant, immunomodulatory, and anti-viral activities. Elementary Chemical Composition Analysis confirmed their structural charac-teristics by Energy Dispersive X-Ray Detector (EDS), Fourier Transform Infrared (FTIR), Nuclear Magnetic Resonance (NMR), and Scanning Electron Microscopy (SEM) techniques. The best DPPH scavenging potential was achieved for LLaEPS-C for concentrations lower than 200mg/mL. LaEPS-C showed moderate antiproliferative activity for the NCI-ADR/RES cell (GI50 65.3 µg/mL), and BLaEPS-C showed weak activity for the K562 cell (GI50 235 µg/mL). LLaEPS-C had a reduced Prothrombin Time (PT) and a procoagulant effect. LLaEPS-C and BLaEPS-C are inducers of pro-inflammatory activity due to their ability to induce TNF-α in human macrophages. LLaEPS-C also showed anti-hRSV, which confirmed the antiviral activity of this molecule.
Chemistry and Materials Science, Organic Chemistry
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