Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Preferential Recruitment of Monocyte into the Brain is Orchestrated by VCAM-1 Expression

Version 1 : Received: 5 June 2023 / Approved: 8 June 2023 / Online: 8 June 2023 (14:02:33 CEST)

How to cite: Zhu, W.-W.; Wang, J.; Dong, Q.; Zhao, L.; Chen, J. Preferential Recruitment of Monocyte into the Brain is Orchestrated by VCAM-1 Expression. Preprints 2023, 2023060649. https://doi.org/10.20944/preprints202306.0649.v1 Zhu, W.-W.; Wang, J.; Dong, Q.; Zhao, L.; Chen, J. Preferential Recruitment of Monocyte into the Brain is Orchestrated by VCAM-1 Expression. Preprints 2023, 2023060649. https://doi.org/10.20944/preprints202306.0649.v1

Abstract

The restriction of normal blood flow is the cause of many diseases including stroke and coronary artery diseases. To study the consequences of vessel blockade, previous models mainly focused on major arteries and have been well studied. However, the sequela from interruption of capillary vessels by microemboli was less well characterized. In this study, we exploited polystyrene microspheres as a mimicry of microemboli and found that microspheres of this size can be trapped in capillary vessels of all organs without causing apparent acute morbidities of the host. Interestingly, we accidentally found significantly increased recruitment of monocyte to the brain vasculature expressing low levels of Ly6C expression, but not to other organs. Further study revealed the spleen is the major origin of the recruited monocyte. Most importantly, VCAM-1 which is constitutively expressed on mouse brain vasculature orchestrates the recruitment of monocyte. Blockade of VCAM-1 in mice can substantially reduce monocyte recruitment. Interestingly, monocytes get activated through TNF- signaling which likely happens in the spleen instead of the brain. Collectively, we found a unique monocyte recruitment strategy in the brain comparing to other orangs, in response to capillary blockade induced by polystyrene microspheres.

Keywords

monocyte, VCAM-1, microsphere, capillary vessel, microemboli

Subject

Biology and Life Sciences, Immunology and Microbiology

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.