Shuwen, L.; Hong, X.; Yinglong, C.; Wei, J.; Di, D.; MengDi, W.; Tiejun, Z.; Liebao, H.; Yuehui, C. MtTGA1 Transcription Factor Enhances Salt Tolerance through Hormonal Regulation and Antioxidant Enzyme Activity in Medicago truncatula. Preprints2023, 2023060691. https://doi.org/10.20944/preprints202306.0691.v1
APA Style
Shuwen, L., Hong, X., Yinglong, C., Wei, J., Di, D., MengDi, W., Tiejun, Z., Liebao, H., & Yuehui, C. (2023). MtTGA1 Transcription Factor Enhances Salt Tolerance through Hormonal Regulation and Antioxidant Enzyme Activity in <em>Medicago truncatula</em>. Preprints. https://doi.org/10.20944/preprints202306.0691.v1
Chicago/Turabian Style
Shuwen, L., Han Liebao and Chao Yuehui. 2023 "MtTGA1 Transcription Factor Enhances Salt Tolerance through Hormonal Regulation and Antioxidant Enzyme Activity in <em>Medicago truncatula</em>" Preprints. https://doi.org/10.20944/preprints202306.0691.v1
Abstract
The TGACG motif-binding factor1 (TGA1) transcription factor, a significant protein in the bZIP transcription factor family, exhibits a myriad of functions, contributes to numerous biological events, and holds substantial application potential. In this study, our analysis revealed a diverse range of photoregulatory and hormone regulatory elements within the MtTGA1 promoter region. The expression profile of MtTGA1 indicated its highest expression in the root, with its regulation influenced by SA, ABA, BR, and GA. Under salt stress conditions, transgenic plants demonstrated significantly longer root lengths and heightened activities of antioxidant enzymes such as ascorbic acid catalase (APX), peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD) in their roots and leaves compared to control plants. Simultaneously, the levels of endogenous hormones ABA and BR escalated in transgenic plants, with a marked change in the morphology of their leaf cells. Transcriptome analysis identified a total of 193 differentially expressed genes, implicating a wide array of biological processes. Furthermore, we found that MtTGA1 is able to interact with Medicago truncatula SPX domain containing protein 1 (SPX1). In conclusion, the MtTGA1 transcription factor plays a crucial role in enhancing salt tolerance, which provides new insights for improving plant survival under salt stress conditions.
Keywords
Root length; MtTGA1; Antioxidant Enzyme; hormones; salt stress
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.