Experiment 1
The scores for seven emotions, neutral, happy, sad, angry, surprised, scared and disgusted, from the FaceReader outputs for one-shot images taken after the presentation of taste stimuli are shown in
Figure 1. The FaceReader output contains ‘contempt’, but in the present study this term was omitted because contempt is not related with the taste evaluation and actually no participants showed this emotion to any taste stimuli tested.
Figure 1 denotes the hedonic pattern profiles across the seven different emotions in response to 10 taste stimuli in 16 participants. The panels are arranged according to the mean perceived hedonic rating for each stimulus in 16 participants.
Figure 2 shows the boxplot analysis of the scores for the seven emotions shown in
Figure 1. The median and interquartile range with minimum and maximum scores are illustrated for the emotions evoked by the 10 stimuli. The profiles of scores for the seven emotions suggested that higher concentrations (10% and 20%) of sucrose showed a strong happiness component, whereas the sadness component was the largest for 1% citric acid, 5% NaCl, and 0.01% QHCl; the neutral component was the largest for the remaining stimuli.
Figure 3 shows the calculated correlation coefficients among profiles of emotional scores for 10 stimuli. We used Spearman’s analysis because scores did not show normal distribution in some of the stimuli. Statistically highly significant correlations were detected in three groups. The first group consisted of 20%, 10% and 5% sucrose (
Figure 2-A to C, respectively) with highly positive perceived hedonic ratings. The second group consisted of 2% MSG, 0.5% MSG and 1% NaCl (
Figure 2-E to G, respectively) with nearly neutral to slightly negative perceived ratings. The third group consisted of 1% citric acid, 5% NaCl and 0.01% QHCl (
Figure 2-H to J, respectively) with highly negative hedonic ratings. It is noted here that 1% NaCl is also correlated well with 1% citric acid and 5% NaCl in their emotional scores (
Figure 2-G to I, respectively).
A multiple linear regression analysis was performed to predict hedonic ratings based on the scores of the seven emotions for one-shot images obtained in Experiment 1. In addition to the data obtained in 16 participants as shown in
Figure 1 and
Figure 2, the data in 13 participants tested with 5% glucose, 0.3% sodium guanylate and 3% NaCl were combined. Before the analysis, multicollinearity was ensured by examining the correlation coefficients among the seven emotions. No statistically significant (
P < 0.05) correlation was found for any pair of the seven emotions. As a result, we obtained the following regression formula for one-shot images [F (7, 191) = 3.513,
P < 0.001 with an adjusted R
2 of 0.614]:
Hedonic rating = 4.914 × surprise + 7.568 × happiness + 0.617 × scare + 4.393 × neutral – 1.402 × disgust – 2.414 × sadness – 3.917 × angry – 3.234
, where happiness (P < 0.001), neutral (P < 0.01), sadness (P < 0.05) and surprise (P < 0.05) were significant predictors of hedonic ratings.
For 2-sec images, a regression formula [F (7, 191) = 4.781, P < 0.001 with an adjusted R2 of 0.474] was obtained for the mean emotion scores:
Hedonic rating = 6.701 × surprise + 7.461 × happiness + 1.657 × scare + 3.701 × neutral – 1.776 × disgust – 2.563 × sadness – 3.488 × angry – 3.007
, where happiness (P < 0.001), neutral (P < 0.05) and surprise (P < 0.05) were significant predictors of hedonic ratings, and sadness was marginally significant (P = 0.061).
For 4-sec images, a regression formula [F (7, 191) = 5.788, P < 0.001 with an adjusted R2 of 0.365] was obtained for the mean emotion scores:
Hedonic rating = 8.276 × surprise + 7.401 × happiness + 0.452 × scare + 3.343 × neutral – 2.022 × disgust – 2.126 × sadness – 1.155 × angry – 3.008
, where happiness (P < 0.001) and surprise (P < 0.05) were significant predictors of hedonic ratings, and neutral was marginally significant (P = 0.075).
For 6-sec images, a regression formula [F (7, 191) = 6.236, P < 0.001 with an adjusted R2 of 0.316] was obtained for the mean emotion scores:
Hedonic rating = 9.714 × surprise + 7.202 × happiness – 1.935 × scare + 3.284 × neutral – 2.201 × disgust – 1.558 × sadness – 1.268 × angry – 3.030
, where happiness (P < 0.001) and surprise (P < 0.01) were significant predictors of hedonic ratings.
Experiment 2
The validity of these formulae was examined by applying the obtained emotion scores to another 11 taste stimuli in a different group of 20 participants who had not been exposed to the formulae in Experiment 1. We investigated the correlation between the estimated ratings and the perceived ratings, as well as how well the estimated ratings matched the perceived ratings for each taste stimulus. In four participants, however, there was an apparent discrepancy between the estimated ratings and the perceived ratings, mainly due to the lack of happiness reported for hedonically positive stimuli such as peach juice and noodle stock (
Figure 4-A). Therefore, the subsequent analyses focused on the remaining 16 participants who exhibited a strong correlation and good agreement between the estimated and perceived ratings (
Figure 4-B). However, a significant difference was observed between the two sets of ratings for the highly palatable peach juice and the highly aversive SOA (Wilcoxon signed-rank test, P < 0.01). This difference in ratings for SOA and peach juice may have been attributed to the limitation of the predicted ratings, which could not reach the maximum hedonic ratings of either –5 or +5. To address this issue, the calculated ratings for peach juice and SOA were multiplied by 1.6. This coefficient was determined based on the ratio between the perceived and calculated ratings for peach juice (4.1/2.6 = 1.58) and SOA (-4.5/-2.7 = 1.67). After this adjustment, the estimated ratings aligned much better with the perceived ratings, with the slope of the regression line improving from 0.697 to 0.891, respectively (
Figure 4-C).
In
Figure 5, the correlation analysis between the mean perceived ratings and mean predicted ratings was depicted, which were calculated using the regression formulae based on the mean emotional scores for different analysis periods: one-shot ± 1 sec (2-sec image) (
Figure 5-A), one-shot ± 2 sec (4-sec image) (
Figure 5-B), and one-shot ± 3 sec (6-sec image) (
Figure 5-C). We utilized Pearson’s correlation analysis since the data for both the calculated and perceived ratings exhibited a normal distribution (Kolmogorov-Smirnov’s test). Although the correlation coefficient slightly decreased from 0.977 to 0.970 to 0.962 as the analysis period increased from 2-sec to 4-sec to 6-sec images, respectively, the slope of the regression line changed from 0.618 to 0.534 to 0.495, indicating a decrease in concordance between the two sets of ratings with longer analysis time.
In addition to these correlation analyses, we examined the concordance between predicted and perceived ratings for each taste stimulus. The difference between the estimated and calculated ratings for each taste stimulus could serve as an indicator of the level of agreement between the two ratings. The mean difference of ratings for all 11 stimuli was calculated to be 0.606, 0.722, 0.953, and 1.027 for one-shot, 2-sec, 4-sec, and 6-sec images, respectively. One-way ANOVA showed a significant main effect [F (3,30) = 13.702, P < 0.001], and post hoc analysis using the Bonferroni test revealed that the difference in ratings for one-shot images was not significantly different from that of 2-sec images but was significantly smaller (P < 0.001) than that of 4-sec and 6-sec images.
Finally, we summarized the time point at which the one-shot image was taken. As depicted in
Figure 6, the time point varied depending on the perceived hedonic value of the stimulation, either before or after the participant's brief remark. One-shot images were more frequently taken after remarks for hedonically positive stimuli such as peach juice and noodle broth, while they were more frequently taken before remarks for hedonically negative stimuli such as 2.5% salt and 1% malic acid. This tendency was statistically significant when Pearson’s correlation coefficient was calculated between the number of one-shot images taken before the remarks and the perceived hedonic values (r = 0.830 and r = 0.973 when SOA was omitted). The same tendency was observed in the data from Experiment 1.
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