The analysis of land cover from 1980 to 2018 showed that the RCLT remained mostly stable at 86% over time. However, in 38 years, the evaluation of the spatio-temporal dynamics of the vegetation of the RCLT has revealed the regression of gallery forests and mosaics of seasonally flooded dense forests, which occupied almost 13% of the RCLT in favor of savannah formations (grassy, tree, shrub and slash and burn). These results are similar to those obtained by Taibou Ba [
10]), in the Saloum delta where they present the conversion of open and dry forests to savanna formations between 1979 and 1999. This dynamic of land cover in the center of Togo was also presented [
12]. In 24 years, the area of open and dry forests has been degraded and has decreased by 157,988 ha, representing a regression rate of 23.44%. The reasons that explain these changes to the environment are identical regardless of the sites: probably in part climate change but also and above all the intense and permanent exploitation of natural resources by man who systematically sets fire to this area to recover some fish in small permanent ponds or in the process of drying up but difficult to access. In the LTCR, forests provide many ecosystem goods and services that benefit all people in and around the LTCR villages. In addition to Non-Timber Forest Products (NTFPs), forests are spiritual places for communities [
5]). Many indigenous communities (pygmies) present in the villages of the LTCR have lifestyles entirely dependent on the forest. Certain forest species used for pharmacopoeia, wood-energy and materials for various activities (construction, crafts, etc.) are regularly extracted in the gallery and dense flood-prone forests encountered in the LTCR. The practice of slash-and-burn agriculture. The conditions of exploitation of forest areas sometimes completely flooded in the rainy season, impose a considerable effort in the dry season on the communities of the RCLT, who are culturally fishermen, to develop them. In order to capitalize on the effort, they proceed to the use of bush fires to recover some fish and create agricultural areas. The extensive slash-and-burn agriculture forces farmers to clear several hectares each year [
12]. This causes the degradation of the plant cover and therefore the impoverishment of the soil. The increase in population density could be a driving force of this degradation which does not exclusively affect the terrestrial forest environment, but also the aquatic environment. The fish resources of the RCLT as well as the large terrestrial fauna regulate their lifestyles according to the forest environment. The edge that comes from the decomposition of the organic matter of dead trees or parts of trees (leaves, bark, branches, roots and fruits) contributes to strengthening the aquatic food chain on which biodiversity in general and ichthyology in particular strongly depends. By destroying gallery forests through agricultural practices based on the use of bush fires, certain species of fish whose feeding and reproduction are conditioned by the presence of a ligneous support (tree roots) will hardly ensure the survival of their species. This observation was made during the study period when the most minimal fishing performance both in terms of diversity and quantity of catches were recorded in the villages of the RCLT located in the North axis. where gallery forests have been converted into shrubby savannah by bush fires, notably in Epéna, Matoko Botakola and towards Mokengui. This corroborates the analysis of the spatio-temporal dynamics which revealed that the gallery forests are entirely converted between 1980, 2000 and 2018 into herbaceous, tree, shrub and slash and burn savannahs, to the detriment of the sustainability and durability of the activity. fishing which is the main activity of the communities of the villages of the RCLT. The fisheries of the Likouala River with herbs commonly called "spawning areas" were diagnosed during the period from July to September 2019. A total of 204 spawning areas (i.e. 162 accessible areas and 42 prohibited areas) were identified in the 07 pilot villages of the study in 3 LTCR Fish Basins. Overall, the ecological characteristics of these spawning grounds do not differ significantly from one station to another. The previous studies clearly show that the physico-chemical parameters of the waters of the Likouala aux Herbes are substantially identical from upstream to downstream [
3,
4,
5]. This further reinforces the results recorded during the study period in the various villages of the RCLT. The sampling stations were compared with each other based on each hydromorphological parameter measured, independently of other factors between July and September 2019. The variation in the width of the Likouala aux herbes river changes from upstream to downstream. Thus, the Bouanela and Mossengue stations have greater values of the bed width of the Likouala river with herbs. On the other hand, the depth does not vary significantly from one station to another. the Likouala aux Herbes River is a watercourse with a very low gradient due to the topography of the RCLT [
3]. This could justify the practically identical value (3.43 ± 0.73 at Mokengui in the north of the LTCR and 3.30 ± 0.78 in the south at Bouanela) of the depth over the entire extent of the waters of the Likouala aux Herbes in the LTCR. However, width and depth are two hydromorphological parameters that contribute to the diversification of ichthyofauna in ichthyological hydrosystems. Several studies carried out on different hydrosystems have already established relationships between species and environmental variables. Distribution of Mormyridae in the Yoko River at Kisangani in the DRC was strongly correlated with a large width of the stream as well as the depth [
2]. According to ([
2] et [
11]) the average water temperature in a rainforest stream rarely exceeds 24°C’. The average temperature recorded during the water sampling campaigns in the various pilot villages was mostly high in the Reserve with values that fluctuated around 28°C with the exception of the Mokengui station which recorded 25°C. The similar values for the temperature of 27.3°C on Likouala with herbs was obtained by Mamonekene [
3]. The high rainfall gallery forest with shading caused by contiguous treetops that run along the river may be the source of this value which supports assertion regarding the impact of forest vegetation cover on temperature variation in the Congo Basin [
2]. The waters of the Likouala aux Herbes are acidic with pH values hovering around 4 [
3]. The results obtained during the study are identical to those of Mamonekene with values of 4.2 ± 0.42 to Mokengui and 4.35 ± 0.13 in Bouanela [
3].The putrefaction of organic matter resulting from the continuous decomposition of drained plants (mudflat, wood debris, tree fruits, helophytes and macrophytes) all along the river contributes through oxidation-reduction reactions to reduce the pH waters of the river). The conductivity at 25°C recorded during the data collection campaign in the pilot villages of the RCLT, show higher values in the North of the reserve (90.40 μS/cm ± 27.65 in Mokengui and relatively low values in Bouanela in the South of the RCLT (58.20 μS/cm ± 24.50).These values are close whose conductivity values at 25°C ranged from 56.5 μS/cm to 84.93 μS/cm [
3]. The type of substrate encountered in the different stations of the Likouala river with herbs between July and September 2019 presents a great diversity from upstream to downstream and is mainly dominated by the mud-sand couple. A study carried out in Pool Malebo in the DRC on the Congo River, of which the Likouala River by the Sangha River is one of the important tributaries in the North of the Republic of Congo, specifies that the diversity of substrates encountered in this part of the Congo River is one of the factors responsible for the diversity of fish species present in the Pool Malebo fishery [
9]. But in addition to this diversity of station substrates, it is also important to mention that the diversity of vegetation mainly encountered in the southern part of the reserve in the pilot villages Mossengue, Botongo, Bouenala whose aquatic vegetation is essentially made up of a variety of partially burned savannah formations with a dominance of macrophytes helophytes, floating hydrophytes, Eichornia crassipes, and fixed Echinochla crassipes and many others also constitute fundamental elements to be taken into consideration in the description of the sampling stations. In short, the physico-chemical characteristics being identical in all the stations, particularly in the spawning grounds, it clearly appears that the diversity of the fish populations would be more linked to the diversity of the microhabitats encountered in the Likouala river with herbs. The interpretation of the data on the physico-chemical and hydromorphological parameters as well as the study of the substrate of each station (Table A5) showed that the stations of the South axis of the RCLT, have in addition a greater width in the area, a diversified aquatic vegetation that colonizes the banks and the bed of the watercourse, fixed or floating on the waters of the Likouala river with herbs. All this diversity of substrates in the waters of these localities greatly contributes to creating micro-habitats favorable to the development of fish species. The more these microhabitats dependent on parameters such as the width of the river bed and the depth are diversified, the more the ichthyofauna of these localities will be diversified. This remark was confirmed, who asserted that in ecoregion 539, tropical floodplain forest rivers consist of diverse micro-habitats, likely to be the source of high biodiversity and endemism [
1]. It is thought that the number of species of Mormyridae inventoried results from this factor. The same observation regarding the distribution of Cyprinidae Labeo has been made in the Malebo pool [
9]. Their distribution is more related to the diversity of micro-habitats encountered in the study area and whose main substrates were: mud, sand, stone and silt. In axis II of the PCA (Component 2, Figure A2(a)), the distribution of the families of Alestidae and Mormyridae was influenced by the presence of sandy type substrates, foliage and macrophytes coupled with the increase in temperature some water. The presence of Alestidae is correlated with temperature peaks, namely 28.79 ± 1.7°C for the Botongo station and 28.13 ± 1.17°C for the Epena station (Table A4 and Table A5). As for the Mormyridae, the highest diversity indices in this case; 0.6229 for Mossengue and 0.5993 for Botongo were recorded at the stations with the highest temperature peaks. The families of Dichtichodontidae, Hepsetidae, Notopteridae, Anabantidae, Schlibeidae and Polypteridae, show no affinity either with the different substrates recorded or with the physico-chemical parameters of the water in axis III. However, they have affinities with each other, hence their clustering in axis III. This affinity would be linked to the different trophic relationships (predator-prey) that exist between the ecological niches of these different species. In short, the diversity of fish species encountered in the Likouala results from our study of the different micro habitats existing in the bed and outside the bed of the watercourse.