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Association of Depression and Anxiety with Sexual Orientation in a Convenience Sample of Latvian Young Adults

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16 November 2023

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16 November 2023

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Abstract
Background. Depression and anxiety and its association with sexual orientation in Latvia is un-derstudied. Outcomes. Kinsey scale, primary outcomes for mental health disturbances were defined as per-son`s subjective feeling of depression and anxiety. Materials and Methods. Participants aged 18-30 year old (n=503) was asked to do a self-administered anonymous web-based survey regarding social and family status, sexual iden-tification and behaviour, violence experience and mental health information. To describe person`s sexual orientation Kinsey scale was used. Prevalence of depression and anxiety, univariate and multivariate regression analysis was performed to measure relations between investigated fac-tors. Results. A total of 503 participants (i.e., 365 females, 133 males, 5 others) were included in the study. Mean (SD) age of participants was 23.0 (3.7) years. Mean (SD) value on the Kinsey scale was 1.4 (1.8) and median (IQR) value was 1.0 (0-2.0) where 1 is defined as ‘mostly heterosexual, only slightly homosexual’ person and 2 is ‘mostly heterosexual, but more than slightly homosexual’. Prevalence of persons reported violence experience was 20.3%, anxiety 56.3% and depression 37.6%. Logistic regression analysis showed that experience of violence was associated with higher odds of developing anxiety and depression (OR: 2.6 [95% CI: 1.7-4.0] and 2.4 [95% CI: 1.5-3.9], respectively). Being in relationship was associated with higher odds of developing anxiety (OR: 2.8[95% CI: 1.3-6.3]). Male sex and income 1001-2000 euros a month were associated with lower odds of developing anxiety (OR: 0.4 [95% CI: 0.2-0.5] and aOR: 0.3 [95% CI: 0.1-0.8], respectively). Sexual orientation showed no significant value in association with anxiety and depression (OR: 1.1 [95% CI: 1.0-1.2] and OR: 1.1 [95% CI: 1.02-1.2], respectively). Conclusions. In our study, the significant factors for developing depression and anxiety were experiencing violence, being female, being in a relationship, and having no income, while sexual orientation showed no significance in relation to depression and anxiety.
Keywords: 
Subject: Medicine and Pharmacology  -   Psychiatry and Mental Health

1. Introduction

Health is defined by World Health Organization (WHO) as “a state of complete physical, mental and social well-being and not merely the absence of disease or infirmity”. Mental health is integral part of it and affects our emotional, psychological and physical well-being. It impacts how we think, how we act, how we perform at our daily tasks. It is condition which allows us to fulfil our ambitions and plans at highest extent. In 2023 WHO estimated approximately 5% of adults worldwide having depression [1]. Our wellbeing is complex and affected by different everyday experiences such as employment status, income and working conditions [2], lifestyle [3], marital status [4], experience of violence and abuse [5]. It becomes imperative to delve deeper into the intricate relationship between these factors and mental health outcomes, a task that this study seeks to undertake.
According to the Centre for Disease Prevention and Control of Latvia 5.7% Latvians aged 15-74 years old in 2019 marked having depressive symptoms, 8.4% indicated having chronic depression, feeling tension and low mood indicated 54% [6], although the most recent findings by the study published in 2023 suggest that many cases are left undiagnosed and the point prevalence of depression according to the PHQ-9 was 6.4% [7].
2021 survey data give evidence that 25.1% Latvian women aged 18-74 in adulthood had experience of physical or sexual violence, 9% reported having psychological disorders later in life. Respectively 19.5% of Latvian men aged 18-74 in adulthood had experience of physical or sexual violence. In general 37.4% Latvian males and females report having violence experience in their childhood [8].
Depression and anxiety are mental health conditions that affect individuals from all walks of life, and sexual orientation is an important aspect of identity that can influence an individual's mental health. Different studies show that lesbian, gay, bisexual and transgender, queer, intersex and asexual (LGBTQIA+) persons are at higher risk of developing depressive and anxiety symptoms [9,10,11,12]. The Minority Stress Model [13] posits that stigma, prejudice, and discrimination may increase psychological distress in LGBTQIA+ populations. However, the evidence varies across the studies, for example evidence suggests that disparities may vary across gender [14], bisexuality status [15] and different measures of sexual orientation [16]. In this study, we aim to explore the association between sexual orientation and depression and anxiety, and to identify potential factors that may contribute to these mental health outcomes. By examining the intersection of sexual orientation and mental health, we hope to increase our understanding of these important issues and ultimately improve mental health outcomes for all individuals, regardless of their sexual orientation.

2. Materials and Methods

Participants

The target population was Latvian young adults aged 18-30 years old. Study sample consisted of 503 volunteers who participated in a web-based survey. The mean age of responders was 23.0 years (SD 3.7) and the median was 22.0 years (IQR 21.0-25.0). Regarding sex, responses indicated 365 females (72.6%), 133 males (26.4%) and 5 ‘other’ (1.0%). Participants were recruited mainly through social media such as ‘Instagram’ and ‘Facebook’, the survey was also widespread between medical and other faculty students. So, the study collected data in a convenience sample.

Measures

Demographics

Sociodemographic characteristics included information such as sex, age, marital status, children, employment status, level of education and income. Sex was assessed as male, female or other. Age was measured in years from 18 to 30 years. We used four categories to assess marital status: single, divorced, in relationship or married. To assess if person has any children we used ‘yes/no’ answer options. Employment status was assessed with four categories: unemployed, student, maternity/paternity leave and employed. Level of education was initially assessed by using 7 categories: primary school, high school, vocational, bachelor/university degree, unfinished university degree, masters or PhD. For the analysis categories were combined into following – primary, secondary/vocational or university degree/unfinished university degree. To assess the income we used five categories: none, less than 500 euros per month, 500-1000 euros per month, 1001-2000 euros per month than 2001 euros or more per month. We also included information about the family to assess if person was raised in full family, as evidence shows, family structure plays significant role in emotional well-being and has an association with developing depression [17,18,19]. Participants were asked if they grew up in a full family with possible answers: full family, with one parent, no parents. Studies show that growing up with siblings also have an impact on emotional well-being in adulthood, for example being treated worse by father or mother compared to sibling is associated with greater depressive and anxiety symptoms [20]. Therefore, we also wanted to assess if participants grew up with siblings, possible answers were ‘Yes’ or ‘No’.

Sexual orientation

Participants responded to a Kinsey scale [21] using points from 0 to 6, including X as asexual or no sociosexual contacts. Points are represented as following: 0 – exclusively heterosexual; 1 – mostly heterosexual, only slightly homosexual; 2 – mostly heterosexual, but more than slightly homosexual; 3 – equally heterosexual and homosexual; 4 – mostly homosexual, but more than slightly heterosexual; 5 – mostly homosexual, only slightly heterosexual and 6 – exclusively homosexual.
For complete evaluation we included in survey questions about sex of the partner and age of first intercourse. To assess sex of the partner we used categories: no sex, same sex, both sexes or opposite sex. To assess age of the first intercourse we used age in years.

Mental health

To assess persons mental health respondents had to mark if they feel any of following symptoms: anxiety, depression, insomnia, panic attacks, loss of appetite, fear, agitation, or none. Later in the analysis we only used anxiety and depression categories as they were most common.
As evidence shows experience of violence has negative impact on mental health [22,23], we wanted to assess if respondents had experience of violence earlier in life with possible answers ‘Yes’ or ‘No’.

Statistical analysis

Descriptive statistics was used such as proportions for categorical variables and means (with standard deviations (SD)) and medians (with interquartile ranges (IQR)) for continuous variables. Associations between depression, anxiety and independent variables were detected using univariate and multivariate binary logistic regression. Adjustment was performed by all factors significant at univariate analysis at the level of p<0.1 simultaneously. Interaction between independent factors was checked before. Results were assessed as statistically significant if p<0.05.

3. Results

Characteristics of the total sample is summarised in Table 1. Survey results showed that students made total of 151 person (30.0%), majority were employed (n=306; 60.8%). 55.6% of participants were with either finished or unfinished university degree (n=278), others have secondary or vocational education (n=212; 42.4%). Regarding income, 199 participants (39.7%) have income of 500-1000 euros a month, others have either less than 500 euros a month or between 1001 and 2000 euros - 139 (27.7%) and 113 (22.6%) persons respectively. Most of participants were single 235 (46.9%) and in relationship 222 (44.3%).
The mean score on the Kinsey scale was 1.4 (SD 1.4), indicating that the majority of participants identified as mostly heterosexual, only slightly homosexual. The median score was 1.0, with an interquartile range of 0-2.0, demonstrating the diversity in sexual orientation among the participants. The prevalence rates of anxiety and depression among the participants were found to be 56.3% and 37.6%, respectively.
Table 2 presents stratified prevalence of depression and results of univariate and multivariate logistic regression analysis. Univariate analysis showed that four factors are statistically significantly associated with depression – having income more or 2001 euros (vs. no income) increased odds for depression 3.8 times [95% CI 1.03-12.4], being homosexual increased odds 1.1 times [95% CI 1.02-1.2] and having sexual partners of both sexes increased odds 1.6 times [95% CI 1.04-2.6], experience of violence increased odds 2.6 times [95% 1.7-4.0].
After adjustment only association between violence experience and depression remained statistically significant. Experience of violence was associated with increased odds for developing depression [OR 2.4 [95% CI: 1.5-3.9]]. Other factors did not show any significant association.
Table 3 presents the stratified prevalence of anxiety and results of univariate and multivariate logistic regression analysis. Univariate analysis indicated that three factors are statistically significantly associated with the anxiety – being in relationship (vs. being married) increased the odds of anxiety 2.8 times [95% CI 1.3-6.3], violence experience increased the odds 2.4 times [95% CI 1.5-3.9], whereas male gender (vs. female) seemed to be preventive as per the anxiety (OR 0.4 [95% CI 0.2-0.4]).
After adjustment four factors were statistically significantly associated with anxiety, i.e. experience of violence kept higher odds of developing anxiety (OR 2.6 [95% CI: 1.7-4.0]). Being in relationship but not married was associated with higher odds of developing anxiety (OR 2.8[95% CI: 1.3-6.3]). Male sex (vs. female) and income more or 1001-2000 euros (vs. none) a month were associated with lower odds of developing anxiety (OR 0.4 [95% CI: 0.2-0.5] and OR 0.3 [95% CI: 0.1-0.8], respectively).
Sexual orientation showed no significant value in association (as per the multivariate analyses) with anxiety and depression (OR: 1.0 [95% CI: 0.8-1.2] and OR: 1.1 [95% CI: 1.0-1.3], respectively).

4. Discussion

The prevalence rates of anxiety and depression among the participants were found to be 56.3% and 37.6%, respectively. Although this study has a limitation, because the analysis was based on respondents subjective feeling without use of a valid evaluation tool and it is possible that depression rate was thus overestimated. Despite this, these results allow us to get an idea of the substantial burden of mental issues in the young adult population of Latvia. Notably, the study revealed a prevalence rate of 20.3% for individuals who reported experiencing violence. Logistic regression analysis demonstrated significant associations between violence experience and increased odds of developing anxiety and depression. Our findings are consistent to earlier studies, for example to Nedley et al. [24] whose study concluded that violence, in particular sexual abuse, increases the level of depression regardless of sexual orientation. These findings underline the detrimental impact of violence on mental health outcomes and emphasize the importance of addressing and preventing violence within society [25,26].
Furthermore, being in a relationship but not married was associated with higher odds of developing anxiety, as indicated by the logistic regression analysis. This finding suggests that relationship status may contribute to increased psychological distress or anxiety symptoms among young adults [27]. One of the theories that explains why marriage can be a protective factor for mental health is social control theory [28] that emphasise how spouses monitor one another’s behaviour encouraging healthy lifestyle and promoting well-being. On the contrary single people or in relationship but not married usually are living alone and may be less able to monitor their partners behaviour.
In contrast, male sex (vs. female) and an income range of more or 1001-2000 euros per month (vs. no income) were associated with lower odds of developing anxiety. These results suggest potential protective factors associated with being male [29,30]. Men and women have differences on molecular level and one explanation why men are less likely to develop anxiety is that women have higher stress-induced cortisol level [31], also negatively valenced emotional stimuli cause greater activation of the amygdala in females. Even this small increase in amygdala activation in females could lead to greater emotional arousal because their amygdala response would be amplified by their greater amygdala-LC connectivity [30].
Having a higher income is also related with lower odds in relation to developing anxiety symptoms [32,33]. Study by De Castro et al. show that people from low-income countries urban areas has difficulties across various functional domains such as difficulties communicating, with self-care, difficulties making friends which are related to higher odds of developing anxiety and depression [33]. However, it is important to note that further research is needed to explore the underlying mechanisms behind these associations.
Interestingly, the study did not find a significant association between sexual orientation and anxiety or depression. These results differ from previous studies, which reported that all sexual minorities are at higher risk of developing mental disorder [34]. Although, there are contradictions between previous studies as well. For example, Nam et al. [35] study showed that only bisexual individuals has higher odds for developing anxiety, depression and suicidality comparing to heterosexuals and gay/lesbian populations. They suggest that the reason can be a ‘double discrimination’ that bisexuals face. The study by Lefevor et al. [36] showed that when sexual attraction, behaviour, and identity were accounted for, relationship between sexual orientation ‘branchedness/discordance’ and health outcomes disappeared. The present study found may suggest that in previous years stigma on LGBTQIA+ individuals has diminished and doesn’t cause such psychological distress as it used to. However, it is essential to interpret this result cautiously, considering the complexities of mental health and sexual orientation [37].
However, it is crucial to acknowledge certain limitations of the study. The cross-sectional nature of the data restricts the ability to establish causal relationships between variables. Additionally, the reliance on self-reported measures for mental health outcomes and experiences of violence may introduce response biases. The absence of randomization and representativeness in participant selection introduces the possibility of selection bias. Moreover, there is a limitation in the study due to the absence of a validated tool for example PHQ9 [38] and GAD7 [39]. Future research should consider longitudinal designs and utilize validated measures to enhance the robustness of the findings.

5. Conclusions

Our study did not reveal association of higher odds for developing depression and anxiety with any particular sexual orientation. Further research should be done to investigate contributing factors that increase odds of developing depression and anxiety.

Author Contributions

Conceptualisation and design – Marija Pavlukovica, Anatolijs Pozarskis, Anda Kivite-Urtane, Acquisition of data – Marija Pavlukovica, Analysis and interpretation of Data – Marija Pavlukovica, Anatolijs Pozarskis, Anda Kivite-Urtane, Statistical analysis – Anda Kivite-Urtane, Drafting the Article – Marija Pavlukovica , Revising it for Intellectual Content – Anatolijs Pozarskis, Anda Kivite-Urtane, Final approval of the Completed Article – Anatolijs Pozarskis, Anda Kivite-Urtane.

Funding

This research received no external funding.

Institutional Review Board Statement

The study was conducted in accordance with the Declaration of Helsinki, and approved by the Institutional Ethics Committee of Riga Stradins University (2-PĒK-4/26/2022; 13.01.2022).

Informed Consent Statement

Informed consent was obtained from all subjects involved in the study.

Data Availability Statement

The data presented in this study are available on request from the corresponding author. The data are not publicly available due to participants privacy protection.

Acknowledgments

The authors would like to thank all the participants for making this study possible.

Conflicts of Interest

The authors declare no conflict of interest.

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Table 1. Characteristics of the total sample (n=503).
Table 1. Characteristics of the total sample (n=503).
Variables n(%)
Sex Female
Male
Other
365 (72.6)
133 (26.4)
5 (1.0)
Age (years) Min.-max.
Mean (SD)
Median (IQR)
17-51
23.0 (3.7)
22.0 (21.0-25.0)
Marital status Single
Divorced
In relationship
Married
235 (46.9)
14 (2.8)
222 (44.3)
30 (6.0)
Children No
Yes
473 (94.0)
30(6.0)
Employment status Unemployed
Student
Maternity/paternity leave
Employed
44 (8.7)
151 (30.0)
2 (0.4)
306 (60.8)
Education University degree, unfinished university degree
Secondary, vocational
Primary
278 (55.6)

212 (42.4)
10 (2.0)
Income None
<500
500-1000
1001-2000
2001+
34 (6.8)
139 (27.7)
199 (39.7)
113 (22.6)
16 (3.2)
Homosexuality scale Min.-max.
Mean (SD)
Median (IQR)
0
1
2
3
4
5
6
X
0-6
1.4 (1.8)
1.0 (0-2.0)
213 (42.3)
127 (25.2)
40 (8.0)
53 (10.5)
8 (1.6)
16 (3.2)
36 (7.2)
10 (2.0)
Transgender No
Yes
497 (98.8)
6 (1.2)
Sex of the partners No sex
Same sex
Both sexes
Opposite sex
55 (11.0)
35 (7.0)
98 (19.6)
311 (62.3)
Age of first intercourse No sex
10-15
15-20
20+
55 (11.2)
40 (8.2)
351 (71.8)
43 (8.8)
Grown up with both parents No
Yes
152 (30.2)
351 (69.8)
Grown up with siblings No
Yes
111 (22.1)
392 (77.9)
Violence experience No
Yes
401 (79.7)
102 (20.3)
Anxiety No
Yes
220 (43.7)
283 (56.3)
Depression No
Yes
314 (62.4)
189 (37.6)
Table 2. Prevalence of depression, results of univariate and multivariate logistic regression analysis.
Table 2. Prevalence of depression, results of univariate and multivariate logistic regression analysis.
Factor Depression No depression OR 95% CI p aOR* 95% CI p
n % n %
Gender
Other 2 40.0 3 60.0 1.1 0.2-6.7 0.91
Male 50 37.6 83 62.4 1.0 0.7-1.5 0.99
Female 137 37.5 228 62.5 1
Age
Median (IQR) 22.0 21.0-25.5 22.0 20.0-25.0 1.02 1.0-1.1 0.46
Marital status
Single 90 38.3 145 61.7 2.5 1.0-6.3 0.06 2.3 0.8-6.3 0.11
Divorced 6 42.9 8 57.1 3.0 0.8-12.0 0.12 3.3 0.8-13.8 0.11
In relationships 87 39.2 135 60.8 2.6 1.0-6.6 0.05 2.5 0.9-6.8 0.07
Married 6 20.0 24 80.0 1 1
Employment
Unemployed 19 43.2 25 56.8 1.4 0.7-2.7 0.37
Employed 116 37.9 190 62.1 1.1 0.7-1.6 0.66
Maternity/paternity leave 0 0 2 100.0 - - -
Student 54 35.8 97 64.2 1
Children
No 178 37.6 295 62.4 1.0 0.5-2.2 0.92
Yes 11 36.7 19 63.3 1
Education
Primary 6 60.0 4 40.0 2.4 0.66-8.7 0.18 2.4 0.6-9.6 0.21
Secondary, vocational 75 35.4 137 64.6 0.9 0.6-1.3 0.48 0.8 0.5-1.2 0.27
University degree, unfinished university degree 107 38.5 171 61.5 1 1
Income
2001+ 9 56.3 7 43.8 3.8 1.03-12.4 0.045 3.0 0.7-12.0 0.13
1001-2000 36 31.9 77 68.1 1.3 0.6-3.1 0.55 1.3 0.5-3.4 0.61
500-1000 81 40.7 118 59.3 1.9 0.8-4.3 0.12 1.6 0.6-3.9 0.34
<500 53 38.1 86 61.9 1.7 0.7-3.9 0.21 1.5 0.6-3.8 0.35
None 9 26.5 25 73.5 1 1
Homosexuality scale
Median (IQR) 1.0 0.0-3.0 1.0 0.0-2.0 1.1 1.02-1.2 0.02 1.1 1.0-1.3 0.08
Sex of the partners
No sex 23 41.8 32 58.2 1.4 0.8-2.5 0.27 1.3 0.7-2.7 0.42
Same sex 13 37.1 22 62.9 1.1 0.6-2.4 0.72 0.6 0.2-1.8 0.40
Both sexes 45 45.9 53 54.1 1.6 1.04-2.6 0.035 1.0 0.5-1.8 0.95
Opposite sex 106 34.1 205 65.9 1 1
Age at first intercourse
No sex 23 41.8 32 58.2 1.1 0.5-2.5 0.82
10-15 16 40.0 24 60.0 1.0 0.4-2.5 0.97
15-20 126 35.9 225 64.1 0.9 0.4-1.6 0.64
20+ 17 39.5 26 60.5 1
Grown up with both parents
No 66 43.4 86 56.6 1.4 1.0-2.1 0.08 1.5 1.0-2.3 0.07
Yes 123 35.0 228 65.0 1
Grown up with siblings
Yes 148 37.8 244 62.2 1.0 0.7-1.6 0.88
No 41 36.9 70 63.1 1
Violence experience
Yes 57 55.9 45 44.1 2.6 1.7-4.0 <0.001 2.6 1.6-4.3 <0.001
No 132 32.9 269 67.1 1
Table 3. Prevalence of anxiety, results of univariate and multivariate logistic regression analysis.
Table 3. Prevalence of anxiety, results of univariate and multivariate logistic regression analysis.
Factor Anxiety No anxiety OR 95% CI p aOR* 95% CI p
n % n %
Gender
Other 3 60.0 2 40.0 0.9 0.1-5.3 0.9 0.7 0.1-7.1 0.7
Male 50 37.6 83 62.4 0.4 0.2-0.5 <0.001 0.4 0.2-0.6 <0.001
Female 230 63.0 135 37.0 1 1
Age
Median (IQR) 0.9 0.9-1.0 0.06 1.02 1.0-1.1 0.49
Marital status
Single 128 54.5 107 45.5 2.1 0.9-4.5 0.07 2.2 0.9-5.6 0.09
Divorced 6 42.9 8 57.1 1.3 0.4-4.7 0.70 1.4 0.4-5.7 0.61
In relationships 138 62.2 84 37.8 2.8 1.3-6.3 0.01 2.8 1.1-7.0 0.02
Married 11 36.7 19 63.3 1 1
Employment
Unemployed 25 56.8 19 43.2 1.0 0.5-1.9 0.93
Employed 171 55.9 135 44.1 0.9 0.6-1.4 0.73
Maternity/paternity leave 0 0 2 100.0 - - -
Student 87 57.6 64 42.4 1
Children
No 269 56.9 204 43.1 1.5 0.7-3.2 0.28
Yes 14 46.7 16 53.3 1
Education
Primary 8 80.0 2 20.0 3.0 0.6-14.6 0.17 2.6 0.5-13.9 0.25
Secondary, vocational 116 54.7 96 45.3 0.9 0.6-1.3 0.64 0.7 0.4-1.1 0.09
University degree, unfinished university degree 158 56.8 120 43.2 1 1
Income
2001+ 7 43.8 9 56.3 0.5 0.2-1.8 0.32 0.3 0.1-1.2 0.09
1001-2000 48 42.5 65 57.5 0.5 0.2-1.1 0.10 0.3 0.1-0.8 0.02
500-1000 117 58.8 82 41.2 1.0 0.5-2.1 0.99 0.5 0.2-1.3 0.16
<500 89 64.0 50 36.0 1.2 0.6-2.7 0.57 0.8 0.3-2.0 0.63
None 20 58.8 14 41.2 1 1
Homosexuality scale
Median (IQR) 1.1 1.0-1.2 0.14 1.0 0.8-1.2 0.92
Sex of the partners
No sex 30 54.5 25 45.5 1.0 0.6-1.8 0.94 0.5 0.2-1.3 0.14
Same sex 20 57.1 15 42.9 1.1 0.6-2.3 0.73 2.3 0.8-7.3 0.14
Both sexes 62 63.3 36 36.7 1.5 0.9-2.3 0.11 1.5 0.8-2.8 0.19
Opposite sex 168 54.0 143 46.0 1 1
Age at first intercourse
No sex 30 54.5 25 45.5 0.6 0.3-1.3 0.20 - - -
10-15 21 52.5 19 47.5 0.5 0.2-1.3 0.17 0.5 0.2-1.3 0.16
15-20 196 55.8 155 44.2 0.6 0.3-1.2 0.15 0.5 0.2-1.1 0.07
20+ 29 67.4 14 32.6 1 1
Grown up with both parents
No 88 57.9 64 42.1 1.1 0.7-1.6 0.63
Yes 195 55.6 156 44.4 1
Grown up with siblings
Yes 217 55.4 175 44.6 0.8 0.6-1.3 0.44
No 66 59.5 45 40.5 1
Violence experience
Yes 74 72.5 28 27.5 2.4 1.5-3.9 <0.001 2.5 1.4-4.4 0.002
No 209 52.1 192 47.9 1 1
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