Ye, L.; Zhang, Q.-Q.; Lin, S.; Zhang, Q.; Yan, J.; Wu, D.-T.; Liu, S.-X.; Qin, W. A Polysaccharide from Ficus carica L. Exerts Immunomodulatory Activity in Both In Vitro and In Vivo Experimental Models. Foods2024, 13, 195.
Ye, L.; Zhang, Q.-Q.; Lin, S.; Zhang, Q.; Yan, J.; Wu, D.-T.; Liu, S.-X.; Qin, W. A Polysaccharide from Ficus carica L. Exerts Immunomodulatory Activity in Both In Vitro and In Vivo Experimental Models. Foods 2024, 13, 195.
Ye, L.; Zhang, Q.-Q.; Lin, S.; Zhang, Q.; Yan, J.; Wu, D.-T.; Liu, S.-X.; Qin, W. A Polysaccharide from Ficus carica L. Exerts Immunomodulatory Activity in Both In Vitro and In Vivo Experimental Models. Foods2024, 13, 195.
Ye, L.; Zhang, Q.-Q.; Lin, S.; Zhang, Q.; Yan, J.; Wu, D.-T.; Liu, S.-X.; Qin, W. A Polysaccharide from Ficus carica L. Exerts Immunomodulatory Activity in Both In Vitro and In Vivo Experimental Models. Foods 2024, 13, 195.
Abstract
Polysaccharide from Ficus carica L. (FCP) exert multiple biological activities, as a biological macromolecule, the available knowledge about the specific structures and mechanisms of biological activity of purified 'Brunswick' fig polysaccharide is currently limited. In the present study, by chemical purification and characteristics identified via specific instruments, and then the impact of FCP on the immunomodulation activity in vitro and in vivo was examined. Structural char-acteristics showed that the molecular weight of the FCP was determined to be 127.5 kDa, the primary monosaccharides present in the FCP were galacturonic acid (GalA), arabinose (Ara), galactose (Gal), rhamnose (Rha), glucose (Glc), and xylose (Xyl) at a ratio of 0.321:0.287:0.269:0.091:0.013:0.011. Based on the investigation of in vitro immunomodulatory ac-tivity, FCP was found to stimulate the production of NO, TNF-α, and IL-6 and increased the pi-nocytic activity of macrophages. Further analysis revealed that FCP activated macrophages by interacting with toll-like receptor 4 (TLR4). Moreover, the in vivo test results indicate that FCP showed significant increase serum pro-inflammatory factors in immunosuppressed mice. Overall, this study suggests that FCP has the potential to be utilized as a novel immunomodulator in the pharmaceutical and functional food industries.
Biology and Life Sciences, Food Science and Technology
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