Nguyen, H.T.; Falzarano, D.; Gerdts, V.; Liu, Q. Construction and Immunogenicity of SARS-CoV-2 Virus-like Particle Expressed by Recombinant Baculovirus BacMam. Microbiology Spectrum 2024, doi:10.1128/spectrum.00959-24.
Nguyen, H.T.; Falzarano, D.; Gerdts, V.; Liu, Q. Construction and Immunogenicity of SARS-CoV-2 Virus-like Particle Expressed by Recombinant Baculovirus BacMam. Microbiology Spectrum 2024, doi:10.1128/spectrum.00959-24.
Nguyen, H.T.; Falzarano, D.; Gerdts, V.; Liu, Q. Construction and Immunogenicity of SARS-CoV-2 Virus-like Particle Expressed by Recombinant Baculovirus BacMam. Microbiology Spectrum 2024, doi:10.1128/spectrum.00959-24.
Nguyen, H.T.; Falzarano, D.; Gerdts, V.; Liu, Q. Construction and Immunogenicity of SARS-CoV-2 Virus-like Particle Expressed by Recombinant Baculovirus BacMam. Microbiology Spectrum 2024, doi:10.1128/spectrum.00959-24.
Abstract
The pandemic SARS-CoV-2 continues to evolve to give rise to variants of concern that can escape vaccine-induced immunity. As such, more effective vaccines are urgently needed. In this study, we evaluated virus-like particle (VLP) as a vaccine platform for SAS-CoV-2. The spike, envelope, and membrane proteins of the SARS-CoV-2 Wuhan strain were expressed by a single recombinant baculovirus BacMam and assembled into VLPs in cell culture. The morphology and size of the SARS-CoV-2 VLP as shown by transmission electron microscopy was found to be similar to the authentic SARS-CoV-2 virus particle. In a mouse trial, two intramuscular immunizations of the VLP BacMam with no adjuvant elicited spike-specific binding antibodies in both sera and bronchoalveolar lavage fluids. Importantly, BacMam VLP -vaccinated mouse sera showed neutralization activity against SARS-CoV-2 spike pseudotyped lentivirus. Our results indicated that the SARS-CoV-2 VLP BacMam stimulated spike-specific immune responses with neutralization activity.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.