Review
Version 1
Preserved in Portico This version is not peer-reviewed
CRISPR/CAS Genome Editing Technology: Implications And Challenges
Version 1
: Received: 26 January 2024 / Approved: 27 January 2024 / Online: 29 January 2024 (08:26:56 CET)
Version 2 : Received: 11 April 2024 / Approved: 12 April 2024 / Online: 12 April 2024 (08:41:23 CEST)
Version 2 : Received: 11 April 2024 / Approved: 12 April 2024 / Online: 12 April 2024 (08:41:23 CEST)
How to cite: Nazir, T.; A. Mirza, H.; Taha, N. CRISPR/CAS Genome Editing Technology: Implications And Challenges. Preprints 2024, 2024011948. https://doi.org/10.20944/preprints202401.1948.v1 Nazir, T.; A. Mirza, H.; Taha, N. CRISPR/CAS Genome Editing Technology: Implications And Challenges. Preprints 2024, 2024011948. https://doi.org/10.20944/preprints202401.1948.v1
Abstract
Scientists can use the Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) protein 9 system, which is a powerful and flexible tool for changing the genome, to change only certain parts of the genome. This has helped researchers figure out how target genes work in biology and disease. CRISPR/Cas9 is made up of a sequence-specific CRISPR RNA (crRNA) set and a generic Cas9 nuclease. The crRNA set tells Cas9 where to cut DNA and cause double-strand breaks. In the next step of cellular DNA repair, insertions, deletions, and swaps are made at specific spots. For CRISPR/Cas9-mediated DNA cleavage to be specific, the target sequences must match the crRNA, and a protospacer nearby motif must be placed after the target sequences. In this article, we talk about how CRISPR/Cas9 works at the molecular level, what it can be used for, what it can't do, and what its future clinical treatment promise might be.
Keywords
CRISPR-Cas technology; Endonuclease recycling; Genome Editing; Implications
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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