PreprintArticleVersion 2This version is not peer-reviewed
Automated ISSR Fingerprinting is a Cost-effective way to assess Genetic Diversity and Taxonomic Differentiation – a Case Study on the Encephalartos eugene-maraisii Species Complex
Version 1
: Received: 18 April 2024 / Approved: 18 April 2024 / Online: 18 April 2024 (13:38:21 CEST)
Version 2
: Received: 12 July 2024 / Approved: 12 July 2024 / Online: 15 July 2024 (13:00:58 CEST)
How to cite:
Murphy, D.; Barker, N. P.; Frisby, A. Automated ISSR Fingerprinting is a Cost-effective way to assess Genetic Diversity and Taxonomic Differentiation – a Case Study on the Encephalartos eugene-maraisii Species Complex. Preprints2024, 2024041263. https://doi.org/10.20944/preprints202404.1263.v2
Murphy, D.; Barker, N. P.; Frisby, A. Automated ISSR Fingerprinting is a Cost-effective way to assess Genetic Diversity and Taxonomic Differentiation – a Case Study on the Encephalartos eugene-maraisii Species Complex. Preprints 2024, 2024041263. https://doi.org/10.20944/preprints202404.1263.v2
Murphy, D.; Barker, N. P.; Frisby, A. Automated ISSR Fingerprinting is a Cost-effective way to assess Genetic Diversity and Taxonomic Differentiation – a Case Study on the Encephalartos eugene-maraisii Species Complex. Preprints2024, 2024041263. https://doi.org/10.20944/preprints202404.1263.v2
APA Style
Murphy, D., Barker, N. P., & Frisby, A. (2024). Automated ISSR Fingerprinting is a Cost-effective way to assess Genetic Diversity and Taxonomic Differentiation – a Case Study on the Encephalartos eugene-maraisii Species Complex. Preprints. https://doi.org/10.20944/preprints202404.1263.v2
Chicago/Turabian Style
Murphy, D., Nigel Paul Barker and Arnold Frisby. 2024 "Automated ISSR Fingerprinting is a Cost-effective way to assess Genetic Diversity and Taxonomic Differentiation – a Case Study on the Encephalartos eugene-maraisii Species Complex" Preprints. https://doi.org/10.20944/preprints202404.1263.v2
Abstract
Recent technological advancements in conservation genetics and genomics have resulted in diverse tools for aiding conservation of species. The precision and resolution of high throughput sequencing technologies provide valuable insights to aid conservation decisions, but these technologies are often financially unfeasible or unavailable in resource constrained countries. Inter-Simple Sequence Repeat (ISSR) markers, when combined with sensitive automated detection systems, provide a simple, cheap means to investigate genetic diversity and discriminate closely related species. Here we apply this technology to assess genetic diversity and taxonomic delimitation in the Encephalartos eugene-maraisii species complex, a highly threatened, taxonomically dubious group of cycads in South Africa. Our analyses support taxonomic singularity of E. dyerianus, E. dolomiticus and E. eugene-maraisii. Relationships between E. nubimontanus and E. cupidus remain uncertain. E. middelburgensis samples showed no clustering but had poor amplification success. This study demonstrates the suitability of automated ISSR fingerprinting as a method for plant conservation studies, especially in resource-constrained countries, and we make recommendations as to how this methodology can be effectively implemented.
Keywords
cycad; ISSR; conservation genetics; species complex; developing countries; technological impediment
Subject
Biology and Life Sciences, Plant Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
