Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Whey Protein Sodium-Caseinate as a Deliverable Vector for Tea Catechins: In vitro Optimization of Its Bioaccessibility, Bioavailability, and Bioactivity Mode of Actions

Ali Korin
,
Mostafa M Gouda
* ,
Mahmoud Youssef
ORCID logo ,
Eman Elsharkawy
ORCID logo ,
Amgad Albahia
ORCID logo ,
Fuchao Zhan
,
Remah Sobhy
,
Bin Li
*
Version 1 : Received: 26 May 2024 / Approved: 27 May 2024 / Online: 27 May 2024 (17:26:53 CEST)

A peer-reviewed article of this Preprint also exists.

Korin, A.; Gouda, M.M.; Youssef, M.; Elsharkawy, E.; Albahi, A.; Zhan, F.; Sobhy, R.; Li, B. Whey Protein Sodium-Caseinate as a Deliverable Vector for EGCG: In Vitro Optimization of Its Bioaccessibility, Bioavailability, and Bioactivity Mode of Actions. Molecules 2024, 29, 2588. Korin, A.; Gouda, M.M.; Youssef, M.; Elsharkawy, E.; Albahi, A.; Zhan, F.; Sobhy, R.; Li, B. Whey Protein Sodium-Caseinate as a Deliverable Vector for EGCG: In Vitro Optimization of Its Bioaccessibility, Bioavailability, and Bioactivity Mode of Actions. Molecules 2024, 29, 2588.

Abstract

Epigallocatechin gallate (EGCG), the principal catechin in green tea, exhibits diverse therapeutic properties. However, its clinical efficacy is hindered by poor stability and low bioavailability. This study investigated solid particle-in-oil-in-water (S/O/W) emulsions stabilized by whey protein isolate (WPI) and sodium caseinate (NaCas) as carriers to enhance the bioavailability and intestinal absorption of EGCG. Molecular docking revealed binding interactions between EGCG and these macromolecules. The emulsions exhibited high encapsulation efficiencies and augmented EGCG bioaccessibility by 64% compared to free EGCG in a simulated gastrointestinal digestion model. EGCG-loaded emulsions demonstrated minimal cytotoxicity towards Caco-2 cells and facilitated EGCG transport across the intestinal epithelial layer without compromising tight junction integrity. Notably, EGCG-loaded emulsions protected Caco-2 cells against oxidative stress by suppressing intracellular reactive oxygen species generation. The results demonstrate the potential of WPI- and NaCas-stabilized S/O/W emulsions to enhance the bioavailability, intestinal absorption, and bioactivity of EGCG, expanding its applications in functional foods and nutraceuticals.

Keywords

Epigallocatechin gallate; Polyphenol; Bioaccessibility; Molecular Docking; Caco-2 cell assay; Anti-proliferative activity

Subject

Biology and Life Sciences, Food Science and Technology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.