PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
A Chimeric Vaccine Based on a Single Fusion Protein (W-PreS-O) Combining RBDs from Wuhan hu-1 Wild Type and Omicron Shows Protective Effect against Omicron Infection in Syrian Hamsters
Version 1
: Received: 28 May 2024 / Approved: 28 May 2024 / Online: 29 May 2024 (07:24:09 CEST)
How to cite:
Gattinger, P.; Kozlovskaya, L. I.; Lunin, A.; Gancharova, O. S.; Sirazova, D. I.; Apolokhov, V.; Chekina, E. S.; Gordeychuk, I.; Karaulov, A.; Valenta, R.; Ishmukhametov, A. A Chimeric Vaccine Based on a Single Fusion Protein (W-PreS-O) Combining RBDs from Wuhan hu-1 Wild Type and Omicron Shows Protective Effect against Omicron Infection in Syrian Hamsters. Preprints2024, 2024051895. https://doi.org/10.20944/preprints202405.1895.v1
Gattinger, P.; Kozlovskaya, L. I.; Lunin, A.; Gancharova, O. S.; Sirazova, D. I.; Apolokhov, V.; Chekina, E. S.; Gordeychuk, I.; Karaulov, A.; Valenta, R.; Ishmukhametov, A. A Chimeric Vaccine Based on a Single Fusion Protein (W-PreS-O) Combining RBDs from Wuhan hu-1 Wild Type and Omicron Shows Protective Effect against Omicron Infection in Syrian Hamsters. Preprints 2024, 2024051895. https://doi.org/10.20944/preprints202405.1895.v1
Gattinger, P.; Kozlovskaya, L. I.; Lunin, A.; Gancharova, O. S.; Sirazova, D. I.; Apolokhov, V.; Chekina, E. S.; Gordeychuk, I.; Karaulov, A.; Valenta, R.; Ishmukhametov, A. A Chimeric Vaccine Based on a Single Fusion Protein (W-PreS-O) Combining RBDs from Wuhan hu-1 Wild Type and Omicron Shows Protective Effect against Omicron Infection in Syrian Hamsters. Preprints2024, 2024051895. https://doi.org/10.20944/preprints202405.1895.v1
APA Style
Gattinger, P., Kozlovskaya, L. I., Lunin, A., Gancharova, O. S., Sirazova, D. I., Apolokhov, V., Chekina, E. S., Gordeychuk, I., Karaulov, A., Valenta, R., & Ishmukhametov, A. (2024). A Chimeric Vaccine Based on a Single Fusion Protein (W-PreS-O) Combining RBDs from Wuhan hu-1 Wild Type and Omicron Shows Protective Effect against Omicron Infection in Syrian Hamsters. Preprints. https://doi.org/10.20944/preprints202405.1895.v1
Chicago/Turabian Style
Gattinger, P., Rudolf Valenta and Aydar Ishmukhametov. 2024 "A Chimeric Vaccine Based on a Single Fusion Protein (W-PreS-O) Combining RBDs from Wuhan hu-1 Wild Type and Omicron Shows Protective Effect against Omicron Infection in Syrian Hamsters" Preprints. https://doi.org/10.20944/preprints202405.1895.v1
Abstract
Omicron SARS-CoV-2 variants have dominated the strains of COVID-19 circulating worldwide for the last two years. Although the mortality rate of the COVID-19 Omicron variants is only approximately half that of previous variants, vaccines effective against Omicron are needed. Demonstrating that a SARS-CoV-2 vaccine can protect against Omicron in an in vivo infection model, particularly in that of Syrian hamsters, is important for the preclinical characterization of SARS-CoV-2 vaccines. Here, we report on the evaluation of W-PreS-O for its ability to protect Syrian hamsters against infection by Omicron. W-PreS-O is a chimeric vaccine based on a single fusion protein (W-PreS-O), combining RBDs from Wuhan hu-1 wild-type and Omicron adsorbed to aluminum hydroxide. The Syrian hamsters were immunized three times at three-week intervals with W-PreS-O or with aluminum hydroxide (placebo), and then they were infected with Omicron BA.1. Non-infected and non-vaccinated animals served as controls. Neutralizing antibody (nAB) titers, weight, lung symptoms (i.e., edema and pneumonia index), and viral loads, as measured using RT-PCR in the upper and lower respiratory tracts, were determined. In addition, infectious virus titers from the lungs were measured using a plaque-forming assay. W-PreS-O-vaccinated hamsters developed robust nABs against Omicron, showed almost no development of pneumonia, and had significantly reduced infectious virus titers in the lungs. Importantly, the viral loads in the nasal cavities of W-PreS-O-vaccinated hamsters were close to or above the PCR cycle threshold considered to be non-infectious. Our data provide compelling evidence demonstrating that the W-PreS-O vaccine has protective effect against Omicron in a Syrian hamster in vivo infection model. W-PreS-O is therefore a highly promising candidate vaccine for Omicron infections, and it should be further evaluated in clinical studies.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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