Franca-Oliveira, G.; Monreal Peinado, S.; Alves de Souza, S.M.; Kalume, D.E.; Ferraz de Souza, T.L.; Hernández-Ledesma, B.; Martinez-Rodriguez, A.J. Proteomic Characterization of a Lunasin-Enriched Soybean Extract Potentially Useful in the Treatment of Helicobacter pylori Infection. Nutrients2024, 16, 2056.
Franca-Oliveira, G.; Monreal Peinado, S.; Alves de Souza, S.M.; Kalume, D.E.; Ferraz de Souza, T.L.; Hernández-Ledesma, B.; Martinez-Rodriguez, A.J. Proteomic Characterization of a Lunasin-Enriched Soybean Extract Potentially Useful in the Treatment of Helicobacter pylori Infection. Nutrients 2024, 16, 2056.
Franca-Oliveira, G.; Monreal Peinado, S.; Alves de Souza, S.M.; Kalume, D.E.; Ferraz de Souza, T.L.; Hernández-Ledesma, B.; Martinez-Rodriguez, A.J. Proteomic Characterization of a Lunasin-Enriched Soybean Extract Potentially Useful in the Treatment of Helicobacter pylori Infection. Nutrients2024, 16, 2056.
Franca-Oliveira, G.; Monreal Peinado, S.; Alves de Souza, S.M.; Kalume, D.E.; Ferraz de Souza, T.L.; Hernández-Ledesma, B.; Martinez-Rodriguez, A.J. Proteomic Characterization of a Lunasin-Enriched Soybean Extract Potentially Useful in the Treatment of Helicobacter pylori Infection. Nutrients 2024, 16, 2056.
Abstract
Helicobacter pylori infection affects over 50% of the world population and leads to chronic inflammation and gastric disorders, being the main pathogen correlated to gastric cancer development. The increasing on antibiotic resistance levels is a major global concern and alternative treatments are needed. Soybean peptides and other compounds might be an alternative in the treatment to avoid, eradicate and/or control symptoms of H. pylori infection. This study aimed to characterize a lunasin-enriched soybean extract (LSE) by proteomics tools and to evaluate its antioxidant, anti-inflammatory and antibacterial properties against H. pylori infection. By LC-MS/MS analysis, 124 proteins were identified, with the 2S albumin (lunasin and large chain subunits) being the fourth most abundant protein (8.9%). Lunasin consisted of 44 amino acid residues and an intramolecular disulfide bond. LSE at a low dose (0.0625 mg/mL) reduced ROS production in both H. pylori-infected and non-infected AGS gastric cells. This led to a significant reduction of 6.71% in the levels of pro-inflammatory interleukin (IL)-8. LSE also showed antibacterial activity against H. pylori which can be attributed to other soybean proteins and phenolic compounds. Our findings suggest that LSE might be a promising alternative in the management of H. pylori infection and associated symptoms.
Biology and Life Sciences, Food Science and Technology
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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