PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Development of Ex Vivo Analysis for Examining Cell Composition, Immunological Landscape, Tumor and Immune Related Markers in Non-small Cell Lung Cancer
Version 1
: Received: 10 June 2024 / Approved: 11 June 2024 / Online: 12 June 2024 (00:07:34 CEST)
How to cite:
Ufimtseva, E. G.; Gileva, M. S.; Kostenko, R. V.; Kozlov, V. V.; Gulyaeva, L. F. Development of Ex Vivo Analysis for Examining Cell Composition, Immunological Landscape, Tumor and Immune Related Markers in Non-small Cell Lung Cancer. Preprints2024, 2024060733. https://doi.org/10.20944/preprints202406.0733.v1
Ufimtseva, E. G.; Gileva, M. S.; Kostenko, R. V.; Kozlov, V. V.; Gulyaeva, L. F. Development of Ex Vivo Analysis for Examining Cell Composition, Immunological Landscape, Tumor and Immune Related Markers in Non-small Cell Lung Cancer. Preprints 2024, 2024060733. https://doi.org/10.20944/preprints202406.0733.v1
Ufimtseva, E. G.; Gileva, M. S.; Kostenko, R. V.; Kozlov, V. V.; Gulyaeva, L. F. Development of Ex Vivo Analysis for Examining Cell Composition, Immunological Landscape, Tumor and Immune Related Markers in Non-small Cell Lung Cancer. Preprints2024, 2024060733. https://doi.org/10.20944/preprints202406.0733.v1
APA Style
Ufimtseva, E. G., Gileva, M. S., Kostenko, R. V., Kozlov, V. V., & Gulyaeva, L. F. (2024). Development of Ex Vivo Analysis for Examining Cell Composition, Immunological Landscape, Tumor and Immune Related Markers in Non-small Cell Lung Cancer. Preprints. https://doi.org/10.20944/preprints202406.0733.v1
Chicago/Turabian Style
Ufimtseva, E. G., Vadim V. Kozlov and Lyudmila F. Gulyaeva. 2024 "Development of Ex Vivo Analysis for Examining Cell Composition, Immunological Landscape, Tumor and Immune Related Markers in Non-small Cell Lung Cancer" Preprints. https://doi.org/10.20944/preprints202406.0733.v1
Abstract
NSCLC is a very aggressive solid tumor, with a poor prognosis due to post-surgical recurrence. Analysis of the specific tumor and immune signatures of NSCLC samples is a critical step in prognostic evaluation and management decisions for patients after surgery. Routine histological assay has some limitations. Therefore, new diagnostic tools with the capability to quickly recognize NSCLC subtypes and correctly identify various markers are needed. We developed a technique for ex vivo expansion of cancer and immune cells from surgical tumor and lung tissue samples of patients with NSCLC (adenocarcinomas and squamous cell carcinomas) and their examination on ex vivo cell preparations and, parallelly, on histological sections after Romanovsky–Giemsa and immunofluorescent/immunochemical staining for cancer-specific and immune-related markers. As a result, PD-L1 expression was detected for some patients only by ex vivo analysis. Immune cell profiling in the tumor microenvironment revealed significant differences in the immunological landscapes between the patients’ tumors, with smokers’ macrophages with simultaneous expression of pro- and anti-inflammatory cytokines, neutrophils, and eosinophils being the dominant populations. The proposed ex vivo analysis may be used as an additional diagnostic tool for quick examining cancer and immune cells in whole tumor samples and avoiding false negatives in histological assays.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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