Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Metabolomic analysis revealed the absence of the principal antimicrobial compound of Pseudomonas donghuensis P482, 7-hydroxytropolone, under restricted nutrient conditions

Marta Matuszewska
,
Magdalena Rajewska
,
Alessia Staropoli
ORCID logo ,
Francesco Vinale
,
Roberta Marra
ORCID logo ,
Matteo Lorito
,
Michał Puchalski
,
Robert Czajkowski
ORCID logo ,
Sylwia Jafra
* ORCID logo
Version 1 : Received: 13 June 2024 / Approved: 13 June 2024 / Online: 14 June 2024 (11:01:45 CEST)

How to cite: Matuszewska, M.; Rajewska, M.; Staropoli, A.; Vinale, F.; Marra, R.; Lorito, M.; Puchalski, M.; Czajkowski, R.; Jafra, S. Metabolomic analysis revealed the absence of the principal antimicrobial compound of Pseudomonas donghuensis P482, 7-hydroxytropolone, under restricted nutrient conditions. Preprints 2024, 2024060948. https://doi.org/10.20944/preprints202406.0948.v1 Matuszewska, M.; Rajewska, M.; Staropoli, A.; Vinale, F.; Marra, R.; Lorito, M.; Puchalski, M.; Czajkowski, R.; Jafra, S. Metabolomic analysis revealed the absence of the principal antimicrobial compound of Pseudomonas donghuensis P482, 7-hydroxytropolone, under restricted nutrient conditions. Preprints 2024, 2024060948. https://doi.org/10.20944/preprints202406.0948.v1

Abstract

Pseudomonas donghuensis P482 exhibits broad antibacterial and antifungal activity, primarily related to the production of 7-hydroxytropolone (7-HT). However, the restricted nutritional conditions (viz. mineral medium with single carbon source) diminished the antagonistic activity of P482. In this study, we unveil profiles of the secondary metabolites from P482 and its selected mutants, using nutrient-diluted (10% tryptone soy broth, TSB) and nutrient-restricted (M9 with either 0.4% glucose or 0.4% glycerol as a sole carbon source) media to explore differences in the quantity and quality of the secondary metabolites produced. No 7-HT was found to be synthetized by P482 under restricted nutritional conditions. However, both the wild-type and the tested mutants of P482 still did express some antagonistic activity towards the plant pathogenic bacteria tested. Based on these results, we postulate that 7-HT is not the sole antimicrobial compound produced by P. donghuensis P482 and that other antimicrobials may be involved in P482 antagonistic activity under limiting-nutrient conditions.

Keywords

antimicrobials, Pseudomonas donghuensis, metabolomics, 7-hydroxytropolone; single carbon source, secondary metabolites

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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