PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Aedes Mosquito Virome in Southwestern Cameroon: Lack of Core Virome, but a Very Rich and Diverse Virome in Ae. Africanus Compared to Other Aedes Species
Version 1
: Received: 13 June 2024 / Approved: 13 June 2024 / Online: 14 June 2024 (11:25:20 CEST)
How to cite:
Mbigha Donfack, K. C.; De Coninck, L.; Ghogomu, S. M.; Matthijnssens, J. Aedes Mosquito Virome in Southwestern Cameroon: Lack of Core Virome, but a Very Rich and Diverse Virome in Ae. Africanus Compared to Other Aedes Species. Preprints2024, 2024060963. https://doi.org/10.20944/preprints202406.0963.v1
Mbigha Donfack, K. C.; De Coninck, L.; Ghogomu, S. M.; Matthijnssens, J. Aedes Mosquito Virome in Southwestern Cameroon: Lack of Core Virome, but a Very Rich and Diverse Virome in Ae. Africanus Compared to Other Aedes Species. Preprints 2024, 2024060963. https://doi.org/10.20944/preprints202406.0963.v1
Mbigha Donfack, K. C.; De Coninck, L.; Ghogomu, S. M.; Matthijnssens, J. Aedes Mosquito Virome in Southwestern Cameroon: Lack of Core Virome, but a Very Rich and Diverse Virome in Ae. Africanus Compared to Other Aedes Species. Preprints2024, 2024060963. https://doi.org/10.20944/preprints202406.0963.v1
APA Style
Mbigha Donfack, K. C., De Coninck, L., Ghogomu, S. M., & Matthijnssens, J. (2024). Aedes Mosquito Virome in Southwestern Cameroon: Lack of Core Virome, but a Very Rich and Diverse Virome in Ae. Africanus Compared to Other Aedes Species. Preprints. https://doi.org/10.20944/preprints202406.0963.v1
Chicago/Turabian Style
Mbigha Donfack, K. C., Stephen Mbigha Ghogomu and Jelle Matthijnssens. 2024 "Aedes Mosquito Virome in Southwestern Cameroon: Lack of Core Virome, but a Very Rich and Diverse Virome in Ae. Africanus Compared to Other Aedes Species" Preprints. https://doi.org/10.20944/preprints202406.0963.v1
Abstract
In Cameroon, Aedes mosquitoes transmit various arboviruses, posing significant health risks. We aimed to characterize the Aedes virome in southwestern Cameroon and identify potential core viruses which might be associated with vector competence. A total of 398 Aedes mosquitoes were collected from four locations (Bafoussam, Buea, Edea and Yaounde). Aedes albopictus dominated all sites except for Bafoussam where Aedes africanus prevailed. Metagenomic analyses of mosquitoes grouped per species into 54 pools revealed notable differences in eukaryotic viromes between Ae. africanus and Ae. albopictus, with the former exhibiting greater richness and diversity. Thirty-seven eukaryotic virus species from 16 families were identified, including six novel viruses with near complete genome sequences. Seven viruses were further quantified in individual mosquitoes via qRT-PCR. Although none of them could be identified as core viruses, Guangzhou sobemo-like virus and Bafoussam mosquito solemovirus virus were highly prevalent regionally in Ae. albopictus and Ae. africanus, respectively. This study highlights the diverse eukaryotic virome of Aedes species in southwestern Cameroon. Despite their shared Genus, Aedes species exhibit limited viral sharing, with varying viral abundance and prevalence across locations. Ae. africanus, an understudied vector, harbors a rich and diverse virome, suggesting potential implications for arbovirus vector competence.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
