Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Screen and Character a New Iflavirus Virus in the Fruit Tree Pest Pyrops candelaria

Hong Lin
,
Weitao Song
,
Dongmei Ma
,
Chi Yang
ORCID logo ,
Yanfang Yao
,
Renyi Liu
ORCID logo ,
Ling Hao
,
Dandan Wu
,
Shihua Wang
,
Jimou Jiang
,
Jun Xiong
,
Rui Ma
,
Jiajing Xiao
* ,
Zhenhong Zhuang
* ORCID logo
Version 1 : Received: 13 June 2024 / Approved: 14 June 2024 / Online: 14 June 2024 (16:08:03 CEST)

How to cite: Lin, H.; Song, W.; Ma, D.; Yang, C.; Yao, Y.; Liu, R.; Hao, L.; Wu, D.; Wang, S.; Jiang, J.; Xiong, J.; Ma, R.; Xiao, J.; Zhuang, Z. Screen and Character a New Iflavirus Virus in the Fruit Tree Pest Pyrops candelaria. Preprints 2024, 2024061024. https://doi.org/10.20944/preprints202406.1024.v1 Lin, H.; Song, W.; Ma, D.; Yang, C.; Yao, Y.; Liu, R.; Hao, L.; Wu, D.; Wang, S.; Jiang, J.; Xiong, J.; Ma, R.; Xiao, J.; Zhuang, Z. Screen and Character a New Iflavirus Virus in the Fruit Tree Pest Pyrops candelaria. Preprints 2024, 2024061024. https://doi.org/10.20944/preprints202406.1024.v1

Abstract

Pyrops candelaria is one of the common pests of fruit trees, but the research on the pathogenic microorganisms it may carry is very limited. Therefore, it is essential to reveal the pathogenic microbes it carries and their potential hazards. This study found a new virus from the transcriptome of P. candelaria, which was first reported in P. candelaria and named as PyCaV (Pyrops candelaria associated virus). RACE and bioinformatics assay revealed that the full-length of PyCaV is 10855 bp with the polyA tail, containing a single open reading frame (ORF) encoding a polyprotein consisting of 3171 amino acid (aa). The virus has a typical Iflavirus structure, including two rhv domains, a RNA helicase domain (HEL), a 3C cysteine protease domain (Pro), and a RNA-dependent RNA polymerase domain (RdRp). Further phylogenetic analysis revealed that this virus belongs to family Iflaviridae and sequence alignements analysis suggedted PyCaV is new member in unassigned genus of family Iflaviridae. Further in-depth analysis of virus-infection-part showed that PyCaV is distributing in the whole longan lanternfly, including its head, chest, and abdomen, but more PyCaV was identified in the chest. The distribution of PyCaV in different parts of P. candelaria was further explored, which showed that more PyCaV were detected in its piercing-sucking mouthparts and chest viscera. Statistical analysis showed that the PyCaV infection was affected by time and locations.

Keywords

Iflavirus; Pyrops candelaria; virus; insect; distribution

Subject

Biology and Life Sciences, Insect Science

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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