Preprint Review Version 3 Preserved in Portico This version is not peer-reviewed

Future Perspectives on the Application of the Oxford Nanopore® MinION Sequencer in Cultural Heritage Biodeterioration Studies

Version 1 : Received: 20 June 2024 / Approved: 20 June 2024 / Online: 21 June 2024 (07:29:34 CEST)
Version 2 : Received: 13 July 2024 / Approved: 15 July 2024 / Online: 15 July 2024 (10:58:53 CEST)
Version 3 : Received: 30 August 2024 / Approved: 2 September 2024 / Online: 3 September 2024 (09:36:12 CEST)

How to cite: Trovão, J.; Soares, F. Future Perspectives on the Application of the Oxford Nanopore® MinION Sequencer in Cultural Heritage Biodeterioration Studies. Preprints 2024, 2024061478. https://doi.org/10.20944/preprints202406.1478.v3 Trovão, J.; Soares, F. Future Perspectives on the Application of the Oxford Nanopore® MinION™ Sequencer in Cultural Heritage Biodeterioration Studies. Preprints 2024, 2024061478. https://doi.org/10.20944/preprints202406.1478.v3

Abstract

The present work offers a contemporary synthesis of the application of the Oxford Nanopore® MinION within the context of cultural heritage biodeterioration investigations, while also providing a comprehensive discussion of possible future perspectives for their utilization in this research field. Owing to the distinctive characteristics of this device, the past few years have witnessed the consistent increase on the application of this system in a variety of cultural heritage materials. In fact, the technique has been useful to understand microbial biodeteriogens diversity and some of their metabolic and biodeteriogenic features. Considering the immense potential for application of this system, this manuscript explores and discusses further possibilities of the technique, focusing on helping understand critical questions on the cultural heritage biodeterioration area. Its application in various differential contexts, has opened doors for prospective applications on other interesting sub-areas of research meriting future investigations, including: biodeteriogens genome and transcriptome analysis, metatranscriptomics, biodeteriorative metabolism studies, inter and intra kingdom interactions analysis, resistome profiling, object history and context studies, in situ applications, bioprospecting and biotechnology.

Keywords

microbiome; MinION; biodeterioration; -omics; next-generation-sequencing; cultural heritage

Subject

Biology and Life Sciences, Life Sciences

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