Version 1
: Received: 21 June 2024 / Approved: 22 June 2024 / Online: 24 June 2024 (12:28:20 CEST)
How to cite:
Yessentayeva, N. A.; Galiyeva, A. R.; Sadyrbekov, D. T.; Daribay, A. T.; Tazhbayev, Y. M. Central Composite Design for Formulation and Optimization of Rifampicin Loaded Polylactide-Co-Glycolide Nanoparticles. Preprints2024, 2024061644. https://doi.org/10.20944/preprints202406.1644.v1
Yessentayeva, N. A.; Galiyeva, A. R.; Sadyrbekov, D. T.; Daribay, A. T.; Tazhbayev, Y. M. Central Composite Design for Formulation and Optimization of Rifampicin Loaded Polylactide-Co-Glycolide Nanoparticles. Preprints 2024, 2024061644. https://doi.org/10.20944/preprints202406.1644.v1
Yessentayeva, N. A.; Galiyeva, A. R.; Sadyrbekov, D. T.; Daribay, A. T.; Tazhbayev, Y. M. Central Composite Design for Formulation and Optimization of Rifampicin Loaded Polylactide-Co-Glycolide Nanoparticles. Preprints2024, 2024061644. https://doi.org/10.20944/preprints202406.1644.v1
APA Style
Yessentayeva, N. A., Galiyeva, A. R., Sadyrbekov, D. T., Daribay, A. T., & Tazhbayev, Y. M. (2024). Central Composite Design for Formulation and Optimization of Rifampicin Loaded Polylactide-Co-Glycolide Nanoparticles. Preprints. https://doi.org/10.20944/preprints202406.1644.v1
Chicago/Turabian Style
Yessentayeva, N. A., Arailym Turashkyzy Daribay and Yerkeblan Muratovich Tazhbayev. 2024 "Central Composite Design for Formulation and Optimization of Rifampicin Loaded Polylactide-Co-Glycolide Nanoparticles" Preprints. https://doi.org/10.20944/preprints202406.1644.v1
Abstract
The aim of this study was to synthesize and optimize polylactide-co-glycolide (PLGA) nanoparticles loaded with rifampicin (RIF), with a given size and high loading rate, using the central composite design (CCD) method. CCD was used to investigate the influence of independent factors such as PLGA:RIF ratio, type of PLGA, type and concentration of surfactants, power and duration of homogenization, and type of organic solvent and its ratio to the aqueous phase on the dependent physicochemical characteristics of the nanoparticles. The optimized nanoparticles were investigated using scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). Comparative evaluation of the drug release kinetics was carried out using different pH and different setups: flow cuvette (USP 4 apparatus) and Franz diffusion cuvette. In addition, the mucoadhesive properties and mycobacterial activity of PLGA-RIF NPs were studied.
Keywords
Nanoparticles; polylactide-co-glycolide; rifampicin; central composite design; tuberculosis
Subject
Chemistry and Materials Science, Polymers and Plastics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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