Version 1
: Received: 25 June 2024 / Approved: 25 June 2024 / Online: 26 June 2024 (08:05:06 CEST)
How to cite:
House, L. C.; Hasan, A.; Asnayanti, A.; Alrubaye, A.; Pummill, J. F.; Rhoads, D. D. Phylogenomic Analyses of Three Distinct Lineages Uniting Staphylococcus cohnii and Staphylococcus Urealyticus from Diverse Hosts. Preprints2024, 2024061797. https://doi.org/10.20944/preprints202406.1797.v1
House, L. C.; Hasan, A.; Asnayanti, A.; Alrubaye, A.; Pummill, J. F.; Rhoads, D. D. Phylogenomic Analyses of Three Distinct Lineages Uniting Staphylococcus cohnii and Staphylococcus Urealyticus from Diverse Hosts. Preprints 2024, 2024061797. https://doi.org/10.20944/preprints202406.1797.v1
House, L. C.; Hasan, A.; Asnayanti, A.; Alrubaye, A.; Pummill, J. F.; Rhoads, D. D. Phylogenomic Analyses of Three Distinct Lineages Uniting Staphylococcus cohnii and Staphylococcus Urealyticus from Diverse Hosts. Preprints2024, 2024061797. https://doi.org/10.20944/preprints202406.1797.v1
APA Style
House, L. C., Hasan, A., Asnayanti, A., Alrubaye, A., Pummill, J. F., & Rhoads, D. D. (2024). Phylogenomic Analyses of Three Distinct Lineages Uniting Staphylococcus cohnii and Staphylococcus Urealyticus from Diverse Hosts. Preprints. https://doi.org/10.20944/preprints202406.1797.v1
Chicago/Turabian Style
House, L. C., Jeff F Pummill and Douglas Duane Rhoads. 2024 "Phylogenomic Analyses of Three Distinct Lineages Uniting Staphylococcus cohnii and Staphylococcus Urealyticus from Diverse Hosts" Preprints. https://doi.org/10.20944/preprints202406.1797.v1
Abstract
We sequenced and assembled genomes for 17 isolates of Staphylococcus cohnii isolated from osteomyelitis lesions in young broilers from two separate experiments where we induced lameness using a hybrid wire-litter flooring system. Whole genome comparisons using three different methods support a close relationship of genomes from both S. cohnii and Staphylococcus urealyticus. The data support three different lineages, we designated Lineage 1, Lineage 2 and Lineage 3, uniting these two species within an evolving complex. We present evidence for horizontal transfer between lineages of genomic regions from 50-440 kbp. The transfer of a 186 kbp region from Lineage 1 to Lineage 2 appears to have generated Lineage 3. Human-associated isolates appear to be limited to Lineages 2 and 3 but Lineage 2 appears to contain a higher number of human pathogenic isolates. The chicken isolates from our lameness trials included genomically diverse isolates from both Lineage 1 and 2, and isolates from both lineages were obtained from osteomyelitis lesions of individual birds. Our results expand the diversity of Staphylococci associated with osteomyelitis in poultry and suggest a high diversity in the microbiome of day-old chicks. Our data also support a reevaluation and unification of the taxonomic classifications of S. cohnii and S. urealyticus.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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