Version 1
: Received: 28 June 2024 / Approved: 28 June 2024 / Online: 1 July 2024 (03:59:35 CEST)
How to cite:
Oliva, G.; Vigliotta, G.; Di Stasio, L.; Vasca, E.; Castiglione, S. Development of Broad-Range Microbial Minimal Culture Medium for Lanthanide Studies. Preprints2024, 2024062036. https://doi.org/10.20944/preprints202406.2036.v1
Oliva, G.; Vigliotta, G.; Di Stasio, L.; Vasca, E.; Castiglione, S. Development of Broad-Range Microbial Minimal Culture Medium for Lanthanide Studies. Preprints 2024, 2024062036. https://doi.org/10.20944/preprints202406.2036.v1
Oliva, G.; Vigliotta, G.; Di Stasio, L.; Vasca, E.; Castiglione, S. Development of Broad-Range Microbial Minimal Culture Medium for Lanthanide Studies. Preprints2024, 2024062036. https://doi.org/10.20944/preprints202406.2036.v1
APA Style
Oliva, G., Vigliotta, G., Di Stasio, L., Vasca, E., & Castiglione, S. (2024). Development of Broad-Range Microbial Minimal Culture Medium for Lanthanide Studies. Preprints. https://doi.org/10.20944/preprints202406.2036.v1
Chicago/Turabian Style
Oliva, G., Ermanno Vasca and Stefano Castiglione. 2024 "Development of Broad-Range Microbial Minimal Culture Medium for Lanthanide Studies" Preprints. https://doi.org/10.20944/preprints202406.2036.v1
Abstract
Rare Earth Elements (REE), also known as Lanthanides (Ln3+), are a group of 17 elements showing peculiar physical and chemical properties. Unlike technological applications, very little is known about the physiological role and toxicity of Ln3+ on biological systems, in particular on microorganisms (e.g., bacteria), which represent the most abundant domains on our planet. Up to now, very limited studies have been conducted due to Ln3+ precipitation with some anions commonly present in the culture media. Therefore, the development of a minimal medium is essential to allow the study of Ln3+-microbial interactions, limiting considerably the precipitation as insoluble salts. In this regard, a new minimal culture medium capable of solubilizing large amounts of Ln3+, and allowing the growth of different microbial taxa, was successfully developed. Assays have shown that the medium is capable of solubilizing Ln3+ up to 100 times more than other common culture media and allowed the growth of 63 bacteria and 5 fungi. The kinetic growth of one yeast and one gram-positive bacterium has been defined, proving to support superior growth and biomass compared to other commonly used minimal media. Moreover, the sensitivity and uptake/absorption of a Bacillus stratosphericus strain was tested, highlighting its capability to tolerate concentrations up to 10 mM of either Cerium, Gadolinium or Lanthanum and accumulate different quantities of the three.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
