PreprintArticleVersion 1This version is not peer-reviewed
Identification of Some Potential Cellular Receptors and Host Enzymes That Could Potentially Refine the Bovine Coronavirus (BCoV) Replication and Tissue Tropism. A Molecular Docking Study.
Version 1
: Received: 2 July 2024 / Approved: 2 July 2024 / Online: 2 July 2024 (14:41:25 CEST)
How to cite:
Khan, M. Y.; Duraisamy, N.; Shah, A. U.; ElAlaoui, R. N.; Cherkaoui, M.; Hemida, M. G. Identification of Some Potential Cellular Receptors and Host Enzymes That Could Potentially Refine the Bovine Coronavirus (BCoV) Replication and Tissue Tropism. A Molecular Docking Study.. Preprints2024, 2024070213. https://doi.org/10.20944/preprints202407.0213.v1
Khan, M. Y.; Duraisamy, N.; Shah, A. U.; ElAlaoui, R. N.; Cherkaoui, M.; Hemida, M. G. Identification of Some Potential Cellular Receptors and Host Enzymes That Could Potentially Refine the Bovine Coronavirus (BCoV) Replication and Tissue Tropism. A Molecular Docking Study.. Preprints 2024, 2024070213. https://doi.org/10.20944/preprints202407.0213.v1
Khan, M. Y.; Duraisamy, N.; Shah, A. U.; ElAlaoui, R. N.; Cherkaoui, M.; Hemida, M. G. Identification of Some Potential Cellular Receptors and Host Enzymes That Could Potentially Refine the Bovine Coronavirus (BCoV) Replication and Tissue Tropism. A Molecular Docking Study.. Preprints2024, 2024070213. https://doi.org/10.20944/preprints202407.0213.v1
APA Style
Khan, M. Y., Duraisamy, N., Shah, A. U., ElAlaoui, R. N., Cherkaoui, M., & Hemida, M. G. (2024). Identification of Some Potential Cellular Receptors and Host Enzymes That Could Potentially Refine the Bovine Coronavirus (BCoV) Replication and Tissue Tropism. A Molecular Docking Study.. Preprints. https://doi.org/10.20944/preprints202407.0213.v1
Chicago/Turabian Style
Khan, M. Y., Mohammed Cherkaoui and Maged Gomaa Hemida. 2024 "Identification of Some Potential Cellular Receptors and Host Enzymes That Could Potentially Refine the Bovine Coronavirus (BCoV) Replication and Tissue Tropism. A Molecular Docking Study." Preprints. https://doi.org/10.20944/preprints202407.0213.v1
Abstract
Bovine coronavirus (BCoV) is one of the most common pathogens affecting cattle of all ages. BCoV possesses multiple tissue tropism in cattle, particularly in the respiratory and enteric tracts of the affected animals. Viral entry is a crucial step in the replication cycle of all viruses, including BCoV. The process of viral entry requires the orchestration between several proteins from the virus and the host side. The coronavirus spike glycoproteins contributed substantially to other viral infections and the molecular pathogenesis of coronaviruses, including BCoV. Meanwhile, among all corona-viruses, the hemagglutinin esterase enzyme is found in only BCoV and the human corona-virus-OC43 (HCoV-OC43). The viral receptors are among the key players in the viral entry and could explain the viral tropism, at least in part. The presence of some host cell proteases also en-hances the virus replication from the entry until the release of the virus from the host cells. Previous studies have shown that the 9- O-acetylated sialic acids could potentially act as receptors for the BCoV. However, little is known about the BCoV receptors and if other co/receptors might be needed to ensure viral entry to the host cells. In addition to that, the roles of other host cell proteases, such as Furin and TMPRSS2, in BCoV infection have not yet been well studied. The main objectives of the current study are to identify some novel receptors for BCoV and some host cell proteases that might enhance virus replication. We used some in silico docking tools to predict some new BCoV receptors out of some known other coronavirus receptors (ACE-2, NRP1, DPP4, APN, and CEACAM1). Our results postulated the potential actions of the host Furin and TMPRRS2 enzymes in the activation of BCoV-S glycoprotein, which potentially enhances BCoV replication. Our modeling confirms the high binding affinities of both the 9- O-acetylated sialic and the NRP1 with the BCoV-S and BCoV-HE compared to the ACE-2, DPP4, and CEACAM-1, suggesting their roles as receptors for BCoV. Our prediction models show the potential bindings of the Furin and TMPRSS2 to the BCoV-S polybasic cleavage site (RRSRR|A). To our knowledge, this is the first study to investigate the bovine coronavirus interaction with some other potential host receptors, particularly ACE-2, NRP1, DPP4, APN and CEACAM1, TMPRSS2, and the bovine Furin protease.
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
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