He, L.; Shi, X.; Wang, Y.; Huang, S. Improved Strategies for Modeling Sick Sinus Syndrome in Rats: Balancing Efficacy and Animal Care. Preprints2024, 2024070327. https://doi.org/10.20944/preprints202407.0327.v1
APA Style
He, L., Shi, X., Wang, Y., & Huang, S. (2024). Improved Strategies for Modeling Sick Sinus Syndrome in Rats: Balancing Efficacy and Animal Care. Preprints. https://doi.org/10.20944/preprints202407.0327.v1
Chicago/Turabian Style
He, L., Yucheng Wang and Shuwei Huang. 2024 "Improved Strategies for Modeling Sick Sinus Syndrome in Rats: Balancing Efficacy and Animal Care" Preprints. https://doi.org/10.20944/preprints202407.0327.v1
Abstract
Sick sinus syndrome (SSS) is a disease of sinus rhythm abnormalities. This study aimed to establish an SSS model by injecting 3% NaOH into the jugular vein(JV). Sprague-Dawley rats were randomly divided into sham, 3% NaOH, and 10% NaOH groups. Anesthetized rats underwent electrocardiogram recording before right JV exposure. Rats in the experimental groups received NaOH injections at 0.01 ml/s, while the sham group did not. Model success was evaluated after one hour. Heart rate(HR) and rhythm were documented at 1 hour, 1 week, and 2 weeks post-molding, including HE staining, membrane ion transcription and expression, and calcium ion content test. In the 3% NaOH group, the model establishment rate was 87%, and the mortality rate was 13%, whereas in the 10% NaOH group, they were 57% and 43% respectively (P < 0.01). The HR of the 3% NaOH group decreased at multiple time points post-molding (P < 0.001), presenting as arrhythmias such as sinus bradycardia. The JV sustained less damage, with necrosis of P cells, changes in membrane ion channels, and reduced calcium ion distribution. In conclusion, JV injection of 3% NaOH can establish the SSS model and benefit animals.
Medicine and Pharmacology, Cardiac and Cardiovascular Systems
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