Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Impact of Sciatic Nerve Injury on Extracellular Matrix of Lower Limb Muscle and Thoracolumbar Fascia: An Observational Study

Xiaoxiao Zhao
ORCID logo ,
Caterina Fede
ORCID logo ,
Lucia Petrelli
,
Carmelo Pirri
,
Elena Stocco
,
Chenglei Fan
,
Andrea Porzionato
,
Raffaele De Caro
ORCID logo ,
Stefano Masiero
ORCID logo ,
Carla Stecco
* ORCID logo
Version 1 : Received: 3 July 2024 / Approved: 4 July 2024 / Online: 4 July 2024 (05:58:04 CEST)

How to cite: Zhao, X.; Fede, C.; Petrelli, L.; Pirri, C.; Stocco, E.; Fan, C.; Porzionato, A.; De Caro, R.; Masiero, S.; Stecco, C. The Impact of Sciatic Nerve Injury on Extracellular Matrix of Lower Limb Muscle and Thoracolumbar Fascia: An Observational Study. Preprints 2024, 2024070390. https://doi.org/10.20944/preprints202407.0390.v1 Zhao, X.; Fede, C.; Petrelli, L.; Pirri, C.; Stocco, E.; Fan, C.; Porzionato, A.; De Caro, R.; Masiero, S.; Stecco, C. The Impact of Sciatic Nerve Injury on Extracellular Matrix of Lower Limb Muscle and Thoracolumbar Fascia: An Observational Study. Preprints 2024, 2024070390. https://doi.org/10.20944/preprints202407.0390.v1

Abstract

Peripheral nerve injury (PNI) is a complex clinical challenge affecting quality of life. Neurological recovery does not always ensure functional recovery as extracellular matrix (ECM) alterations affecting muscle function. This study evaluates hyaluronan (HA) and collagen concentration in gastrocnemius muscle and thoracolumbar fascia (TLF) in unilateral lower limb PNI rats to ex-plore systemic ECM alterations following PNI and their impacts on functional recovery. Eighteen 8-week-old male Sprague-Dawley rats were divided into experimental (n=12 with left sciatic nerve transection injury repaired by nerve conduit) and control (n=6) groups. After six weeks, motor function was evaluated. Muscle and TLF samples were analyzed for HA and collagen concentrations using Purple-Jelley HA Assay and Total-Collagen Assay kit. HA and collagen distribution was assessed using Alcian Blue and immunohistochemistry, and Picrosirius Red staining. In PNI rats, SFI and gait analysis were -87±9.12 and 1.32±0.29. Both sides of muscles showed significantly lower HA concentration than that in healthy rats (left p

Keywords

extracellular matrix; peripheral nerve injury; hyaluronan; collagen; intramuscular connective tissue; thoracolumbar fascia

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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