Version 1
: Received: 4 July 2024 / Approved: 4 July 2024 / Online: 4 July 2024 (13:24:54 CEST)
How to cite:
Hino, Y.; Arif, M.; Rahman, M. M.; Husuna, A. A.; Hasan, M. N.; Miura, N. Hypoxia-mediated Long Non-coding RNA Fragment Identified in Canine Oral Melanoma through Transcriptome Analysis. Preprints2024, 2024070430. https://doi.org/10.20944/preprints202407.0430.v1
Hino, Y.; Arif, M.; Rahman, M. M.; Husuna, A. A.; Hasan, M. N.; Miura, N. Hypoxia-mediated Long Non-coding RNA Fragment Identified in Canine Oral Melanoma through Transcriptome Analysis. Preprints 2024, 2024070430. https://doi.org/10.20944/preprints202407.0430.v1
Hino, Y.; Arif, M.; Rahman, M. M.; Husuna, A. A.; Hasan, M. N.; Miura, N. Hypoxia-mediated Long Non-coding RNA Fragment Identified in Canine Oral Melanoma through Transcriptome Analysis. Preprints2024, 2024070430. https://doi.org/10.20944/preprints202407.0430.v1
APA Style
Hino, Y., Arif, M., Rahman, M. M., Husuna, A. A., Hasan, M. N., & Miura, N. (2024). Hypoxia-mediated Long Non-coding RNA Fragment Identified in Canine Oral Melanoma through Transcriptome Analysis. Preprints. https://doi.org/10.20944/preprints202407.0430.v1
Chicago/Turabian Style
Hino, Y., MD Nazmul Hasan and Naoki Miura. 2024 "Hypoxia-mediated Long Non-coding RNA Fragment Identified in Canine Oral Melanoma through Transcriptome Analysis" Preprints. https://doi.org/10.20944/preprints202407.0430.v1
Abstract
Hypoxia contributes to tumor progression and metastasis, and hypoxically dysregulated RNA molecules may thus be implicated in poor outcomes. Canine oral melanoma (COM) has a par-ticularly poor prognosis, and some hypoxia-mediated miRNAs are known to exist in this cancer; however, equivalent data on hypoxically dysregulated other non-coding RNAs (ncRNAs) are lacking. Accordingly, we aimed to elucidate non-miRNA ncRNAs that may be mediated by hy-poxia, targeting primary-site and metastatic COM cell lines and clinical COM tissue samples in next generation sequencing (NGS), with subsequent qPCR validation and quantification in COM primary and metastatic cells, plasma and extracellular vesicles (EVs) for any identified ncRNA of interest. Findings suggested that a number of non-miRNA ncRNA species are hypoxically up- or downregulated in COM. We identified one ncRNA, the long ncRNA fragment EN-SCAFT00000084705.1, as a molecule of interest due to its consistent downregulation in COM tissues, hypoxically and normoxically cultured primary and metastatic cell lines, when compared to the oral tissues from healthy dogs. However, this molecule was undetectable in plasma and plasma EVs, suggesting that its expression may be tumor tissue-specific and it has little potential as a biomarker. Here, we provide evidence of hypoxic transcriptional dysregulation for ncRNAs other than miRNA in COM for the first time, and suggest that ncRNA ENSCAFT00000084705.1 is a molecule of interest for future research on the role of the transcriptome in hypoxia-mediated progression of this aggressive cancer.
Keywords
Hypoxia; melanoma; ncRNA; NGS; dog; metastasis
Subject
Medicine and Pharmacology, Veterinary Medicine
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
