Version 1
: Received: 4 July 2024 / Approved: 5 July 2024 / Online: 5 July 2024 (10:45:33 CEST)
How to cite:
Nattich-Rak, M.; Sadowska, M.; Adamczyk, Z.; Basińska, T.; Mickiewicz, D.; Gadzinowski, M. Deposition Kinetics of Human Serum Albumin Functionalized Spheroidal Particles at Abiotic Surfaces: Mimicking Bacteria Behavior. Preprints2024, 2024070525. https://doi.org/10.20944/preprints202407.0525.v1
Nattich-Rak, M.; Sadowska, M.; Adamczyk, Z.; Basińska, T.; Mickiewicz, D.; Gadzinowski, M. Deposition Kinetics of Human Serum Albumin Functionalized Spheroidal Particles at Abiotic Surfaces: Mimicking Bacteria Behavior. Preprints 2024, 2024070525. https://doi.org/10.20944/preprints202407.0525.v1
Nattich-Rak, M.; Sadowska, M.; Adamczyk, Z.; Basińska, T.; Mickiewicz, D.; Gadzinowski, M. Deposition Kinetics of Human Serum Albumin Functionalized Spheroidal Particles at Abiotic Surfaces: Mimicking Bacteria Behavior. Preprints2024, 2024070525. https://doi.org/10.20944/preprints202407.0525.v1
APA Style
Nattich-Rak, M., Sadowska, M., Adamczyk, Z., Basińska, T., Mickiewicz, D., & Gadzinowski, M. (2024). Deposition Kinetics of Human Serum Albumin Functionalized Spheroidal Particles at Abiotic Surfaces: Mimicking Bacteria Behavior. Preprints. https://doi.org/10.20944/preprints202407.0525.v1
Chicago/Turabian Style
Nattich-Rak, M., Damian Mickiewicz and Mariusz Gadzinowski. 2024 "Deposition Kinetics of Human Serum Albumin Functionalized Spheroidal Particles at Abiotic Surfaces: Mimicking Bacteria Behavior" Preprints. https://doi.org/10.20944/preprints202407.0525.v1
Abstract
Human serum albumin (HSA) corona formation at polymer microparticles of a spheroidal shape was studied using dynamic light scattering and laser Doppler velocimetry (LDV). Physicochemical characteristics of the albumin comprising, the zeta potential and the isoelectric point were determined as a function of pH for various ionic strengths. Analogous characteristics of the polymer particles were carried out. The adsorption of albumin on the particles was monitored by electrophoretic mobility measurements (LDV). The stability of the HSA functionalized particle suspensions under various pHs and their electrokinetic properties were determined. The deposition kinetics of the particles on mica, silica and gold sensors were determined in situ by optical microscopy, AFM and by the quartz microbalance (QCM) under diffusion and flow conditions. The obtained results were interpreted in terms of the coarse-grained random sequential adsorption model that allowed to estimate the range of applicability of QCM for determining deposition kinetics of virus and bacteria at abiotic surfaces.
Keywords
albumin coronas at particles; bacteria deposition; deposition of albumin polymer particle conjugates; HSA functionalized particles; spheroidal polymer particles; quartz microbalance measurements; zeta potential of albumin corona
Subject
Chemistry and Materials Science, Nanotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
