Mature MUC5AC Expression in Resected Pancreatic Ductal Adenocarcinoma Predicts the Treatment Response And Outcomes

How to cite: Manne, A.; Esnakula, A. K.; Sheel, A.; Sara, A.; Manne, U.; Paluri, R. K.; He, K.; Yang, W.; Sohal, D. P.; Kasi, A.; Noonan, A. M.; Mittra, A.; Hays, J.; Roychowdhury, S.; Malalur, P.; Rahman, S.; Jin, N.; Cloyd, J. M.; Tsai, S.; Aslam, E.; Pitter, K.; Miller, E.; Thanikachalam, K.; Dillhoff, M.; Yu, L. Mature MUC5AC Expression in Resected Pancreatic Ductal Adenocarcinoma Predicts the Treatment Response And Outcomes. Preprints 2024, 2024070605. https://doi.org/10.20944/preprints202407.0605.v1 Manne, A.; Esnakula, A. K.; Sheel, A.; Sara, A.; Manne, U.; Paluri, R. K.; He, K.; Yang, W.; Sohal, D. P.; Kasi, A.; Noonan, A. M.; Mittra, A.; Hays, J.; Roychowdhury, S.; Malalur, P.; Rahman, S.; Jin, N.; Cloyd, J. M.; Tsai, S.; Aslam, E.; Pitter, K.; Miller, E.; Thanikachalam, K.; Dillhoff, M.; Yu, L. Mature MUC5AC Expression in Resected Pancreatic Ductal Adenocarcinoma Predicts the Treatment Response And Outcomes. Preprints 2024, 2024070605. https://doi.org/10.20944/preprints202407.0605.v1

Cite

Export citation file: Bib TeX | RIS

MDPI and ACS Style

Manne, A.; Esnakula, A. K.; Sheel, A.; Sara, A.; Manne, U.; Paluri, R. K.; He, K.; Yang, W.; Sohal, D. P.; Kasi, A.; Noonan, A. M.; Mittra, A.; Hays, J.; Roychowdhury, S.; Malalur, P.; Rahman, S.; Jin, N.; Cloyd, J. M.; Tsai, S.; Aslam, E.; Pitter, K.; Miller, E.; Thanikachalam, K.; Dillhoff, M.; Yu, L. Mature MUC5AC Expression in Resected Pancreatic Ductal Adenocarcinoma Predicts the Treatment Response And Outcomes. Preprints 2024, 2024070605. https://doi.org/10.20944/preprints202407.0605.v1

APA Style

Manne, A., Esnakula, A. K., Sheel, A., Sara, A., Manne, U., Paluri, R. K., He, K., Yang, W., Sohal, D. P., Kasi, A., Noonan, A. M., Mittra, A., Hays, J., Roychowdhury, S., Malalur, P., Rahman, S., Jin, N., Cloyd, J. M., Tsai, S., Aslam, E., Pitter, K., Miller, E., Thanikachalam, K., Dillhoff, M., & Yu, L. (2024). Mature MUC5AC Expression in Resected Pancreatic Ductal Adenocarcinoma Predicts the Treatment Response And Outcomes. Preprints. https://doi.org/10.20944/preprints202407.0605.v1

Chicago/Turabian Style

Manne, A., Mary Dillhoff and LIanbo Yu. 2024 "Mature MUC5AC Expression in Resected Pancreatic Ductal Adenocarcinoma Predicts the Treatment Response And Outcomes" Preprints. https://doi.org/10.20944/preprints202407.0605.v1

Abstract

Neoadjuvant therapy (NAT) for early-stage pancreatic ductal adenocarcinoma (PDA) has recently gained prominence. We investigated the clinical significance of mucin 5 AC (MUC5AC), which exists in two major glycoforms, a less-glycosylated immature isoform (IM) and a heavi-ly-glycosylated mature isoform (MM), as a biomarker in resected PDA. Immunohistochemistry was performed on 100 resected PDAs to evaluate the expression of IM and MM isoforms of MUC5AC using respective monoclonal antibodies CLH2 (NBP2-44455) and 45M1 (ab3649). MUC5AC localization (cytoplasmic, apical, and extra-cellular, EC) was determined, and H-scores were calculated. Univariate and multivariate (MVA) Cox regression models were used to estimate progression-free survival (PFS) and overall survival (OS). Of 100 resected PDA patients, 43 received NAT, and 57 were treatment-naïve with upfront surgery (UpS). In the study population (N=100), IM expression (objective response vs. no response vs. UpS = H-scores 104 vs. 152 vs. 163, p=0.01) and MM-MUC5AC detection rates (56% vs. 63% vs. 82%, p=0.02) were significantly different. In the NAT-group, MM-MUC5AC-negative patients had significantly better PFS on MVA (Hazard Ratio, 0.2, 95 % CI: 0.059-0.766, p = 0.01). Similar results were noted in a FOLFIRINOX sub-group (N=36). We established an association of MUC5AC expression with treatment response and outcomes.

Keywords

pancreatic adenocarcinoma; MUC5AC; neoadjuvant therapy; prognosis; recurrence; resectable pancreatic cancer; predictive biomarker

Subject

Medicine and Pharmacology, Gastroenterology and Hepatology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

Not displayed online.

Importance: How significant is the paper to the field?

Outstanding/highlight paper

Significant contribution

Incremental contribution

No contribution

I am not qualified to judge

Soundness of evidence/arguments presented:

Conclusions well supported

Most conclusions supported (minor revision needed)

Incomplete evidence (major revision needed)

Hypothesis, unsupported conclusions, or proof-of-principle

I am not qualified to judge

Please leave your comment to this article below.

Mathematical equations can be typed in either LaTeX formats \\[ ... \\] or $$ ... $$, or MathML format <math> ... </math>. Try the LaTeX or MathML example.

Type equation:

Preview:

Copy equation into message below

Close menu

Please click a symbol to insert it into the message box below:

Please enter the link here:

Please enter a valid URL

Optionally, you can enter text that should appear as linked text:

Copy link into message below

Close menu

Please enter or paste the URL to the image here (please only use links to jpg/jpeg, png and gif images):

Please enter a valid URL

Copy image into message below

Close menu

Type author name or keywords to filter the list of references in this group (you can add a new citation under Bibliography):

No existing citations in Discussion Group

Wikify editor is a simple editor for wiki-style mark-up. It was written by MDPI for Sciforum in 2014. The rendering of the mark-up is based on Wiky.php with some tweaks. Rendering of mathematical equations is done with MathJax. Please send us a message for support or for reporting bugs.

Close menu

Please declare any conflicts of interest you may have with this paper, financial or otherwise.

I do not have a conflict of interest

I would like to declare a conflict of interest

Display my name on the website Send my name and email address to the authors

Captcha

Renew

I accept the following conditions:

Comments must follow the standards of professional discourse and should focus on the scientific content of the article. Insulting or offensive language, personal attacks and off-topic remarks will not be permitted. Comments must be written in English. Preprints reserves the right to remove comments without notice. Readers who post comments are obliged to declare any competing interests, financial or otherwise.

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)

* All users must log in before leaving a comment

what’s this?

Add a record of this review to Publons to track and showcase your reviewing expertise across the world’s journals.

Downloads 0

Comments 0

Metrics 0

Get PDF Supplementary Files Cite Share 0

Bookmark BibSonomy Mendeley Reddit Delicious

Alerts

Notify me about updates to this article or when a peer-reviewed version is published.

Preprints.org is a free preprint server subsidized by MDPI in Basel, Switzerland.

MDPI Initiatives

SciProfiles
Sciforum
Encyclopedia
MDPI Books
Scilit
Proceedings
JAMS

Important links

How it Works
Advisory Board
FAQ
Friendly Journals
Instructions for Authors
About
Statistics

Choose the area that interest you and we will send you notifications of new preprints at your preferred frequency.

Disclaimer

We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.

RSS feed for

Preprints: The Multidisciplinary Preprint Platform

Paste the URL in your RSS reader:

https://www.preprints.org/rss

If you want to subscribe our website in subject-wide, you could find the URL by choosing in the following:

https://www.preprints.org/rss

Please note that by using ScienceDirect RSS feeds, you agree to our Terms of Use and Privacy Policy.